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Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange

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Kathryn Browning, T K Lind, S Maric, S Malekkhaiat-Häffner, G N Fredrikson, E Bengtsson, M Malmsten, M Cárdenas

High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the 'good' cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the 'bad' cholesterol, depositing lipid material into the vascular wall.

Original languageEnglish
Article number7478
JournalScientific Reports
Volume7
Issue number1
Number of pages11
ISSN2045-2322
DOIs
Publication statusPublished - 7 Aug 2017

    Research areas

  • Journal Article

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