Glycine Perturbs Local and Global Conformational Flexibility of a Transmembrane Helix
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Glycine Perturbs Local and Global Conformational Flexibility of a Transmembrane Helix. / Högel, Philipp; Götz, Alexander; Kuhne, Felix; Ebert, Maximilian; Stelzer, Walter; Rand, Kasper D; Scharnagl, Christina; Langosch, Dieter.
In: Biochemistry, Vol. 57, No. 8, 27.02.2018, p. 1326-1337.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Glycine Perturbs Local and Global Conformational Flexibility of a Transmembrane Helix
AU - Högel, Philipp
AU - Götz, Alexander
AU - Kuhne, Felix
AU - Ebert, Maximilian
AU - Stelzer, Walter
AU - Rand, Kasper D
AU - Scharnagl, Christina
AU - Langosch, Dieter
PY - 2018/2/27
Y1 - 2018/2/27
N2 - Flexible transmembrane helices frequently support the conformational transitions between different functional states of membrane proteins. While proline is well known to distort and destabilize transmembrane helices, the role of glycine is still debated. Here, we systematically investigated the effect of glycine on transmembrane helix flexibility by placing it at different sites within the otherwise uniform leucine/valine repeat sequence of the LV16 model helix. We show that amide deuterium/hydrogen exchange kinetics are increased near glycine. Molecular dynamics simulations reproduce the measured exchange kinetics and reveal, at atomic resolution, a severe packing defect at glycine that enhances local hydration. Furthermore, glycine alters H-bond occupancies and triggers a redistribution of α-helical and 310-helical H-bonds. These effects facilitate local helix bending at the glycine site and change the collective dynamics of the helix.
AB - Flexible transmembrane helices frequently support the conformational transitions between different functional states of membrane proteins. While proline is well known to distort and destabilize transmembrane helices, the role of glycine is still debated. Here, we systematically investigated the effect of glycine on transmembrane helix flexibility by placing it at different sites within the otherwise uniform leucine/valine repeat sequence of the LV16 model helix. We show that amide deuterium/hydrogen exchange kinetics are increased near glycine. Molecular dynamics simulations reproduce the measured exchange kinetics and reveal, at atomic resolution, a severe packing defect at glycine that enhances local hydration. Furthermore, glycine alters H-bond occupancies and triggers a redistribution of α-helical and 310-helical H-bonds. These effects facilitate local helix bending at the glycine site and change the collective dynamics of the helix.
U2 - 10.1021/acs.biochem.7b01197
DO - 10.1021/acs.biochem.7b01197
M3 - Journal article
C2 - 29389107
VL - 57
SP - 1326
EP - 1337
JO - Biochemistry
JF - Biochemistry
SN - 0006-2960
IS - 8
ER -
ID: 195553900