Fundamental studies on selectivity in 3-phase liquid-phase microextraction (LPME) of basic drugs

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Standard

Fundamental studies on selectivity in 3-phase liquid-phase microextraction (LPME) of basic drugs. / Pedersen-Bjergaard, Stig; Ho, Tung Si; Rasmussen, Knut Einar.

In: Journal of Separation Science, Vol. 25, No. 3, 27.03.2002, p. 141-146.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pedersen-Bjergaard, S, Ho, TS & Rasmussen, KE 2002, 'Fundamental studies on selectivity in 3-phase liquid-phase microextraction (LPME) of basic drugs', Journal of Separation Science, vol. 25, no. 3, pp. 141-146. https://doi.org/10.1002/1615-9314(20020201)25:3<141::AID-JSSC141>3.0.CO;2-3

APA

Pedersen-Bjergaard, S., Ho, T. S., & Rasmussen, K. E. (2002). Fundamental studies on selectivity in 3-phase liquid-phase microextraction (LPME) of basic drugs. Journal of Separation Science, 25(3), 141-146. https://doi.org/10.1002/1615-9314(20020201)25:3<141::AID-JSSC141>3.0.CO;2-3

Vancouver

Pedersen-Bjergaard S, Ho TS, Rasmussen KE. Fundamental studies on selectivity in 3-phase liquid-phase microextraction (LPME) of basic drugs. Journal of Separation Science. 2002 Mar 27;25(3):141-146. https://doi.org/10.1002/1615-9314(20020201)25:3<141::AID-JSSC141>3.0.CO;2-3

Author

Pedersen-Bjergaard, Stig ; Ho, Tung Si ; Rasmussen, Knut Einar. / Fundamental studies on selectivity in 3-phase liquid-phase microextraction (LPME) of basic drugs. In: Journal of Separation Science. 2002 ; Vol. 25, No. 3. pp. 141-146.

Bibtex

@article{bb162fa7b8364d299a28ab3150e63aac,
title = "Fundamental studies on selectivity in 3-phase liquid-phase microextraction (LPME) of basic drugs",
abstract = "Recently, we introduced an inexpensive and disposable hollow fibre based device for liquid-phase microextraction (LPME) where ionic analytes typically are extracted from 1-4 mL aqueous samples (such as plasma and urine) through an organic solvent immobilized in the pores of a polypropylene hollow fibre and into a 10-25 μL volume of acceptor phase present inside the hollow fibre. Because of the substantial volume ratio of the sample relative to the acceptor phase, ionic analytes may be preconcentrated considerably during LPME (typical preconcentration factors of 50 to 150). In addition, during LPME from biological samples such as plasma and urine, the majority of matrix components are not extracted into the acceptor phase resulting in excellent sample clean-up. For the extraction of basic drugs, dilute solutions of HCl or acidic phosphate buffers have been utilized as acceptor phase without further optimization. In the present work therefore, systematic studies have been performed to explore new acidic acceptor phase possibilities in combination with dihexyl ether as a standard organic solvent in the pores of the hollow fibre. In general, 10 mM solutions of HCl, H2SO4, and phosphate pH 3.3 were found to provide the highest extraction recoveries. pH was found to play a major role in adjusting the selectivity of LPME, whereas minor selectivity alterations were observed on changing the type of acid in the acceptor phase. For weakly basic drugs, an increased level of HCl in the acceptor phase may provide higher recovery, and for certain drugs, addition of ethanol to the acceptor phase may improve the extractability.",
keywords = "3-Phase extraction, Basic drugs, Liquid-phase microextraction (LPME), Porous hollow fibres, Selectivity",
author = "Stig Pedersen-Bjergaard and Ho, {Tung Si} and Rasmussen, {Knut Einar}",
year = "2002",
month = mar,
day = "27",
doi = "10.1002/1615-9314(20020201)25:3<141::AID-JSSC141>3.0.CO;2-3",
language = "English",
volume = "25",
pages = "141--146",
journal = "HRC &amp; CC, Journal of High Resolution Chromatography and Chromatography Communications",
issn = "1615-9306",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "3",

}

RIS

TY - JOUR

T1 - Fundamental studies on selectivity in 3-phase liquid-phase microextraction (LPME) of basic drugs

AU - Pedersen-Bjergaard, Stig

AU - Ho, Tung Si

AU - Rasmussen, Knut Einar

PY - 2002/3/27

Y1 - 2002/3/27

N2 - Recently, we introduced an inexpensive and disposable hollow fibre based device for liquid-phase microextraction (LPME) where ionic analytes typically are extracted from 1-4 mL aqueous samples (such as plasma and urine) through an organic solvent immobilized in the pores of a polypropylene hollow fibre and into a 10-25 μL volume of acceptor phase present inside the hollow fibre. Because of the substantial volume ratio of the sample relative to the acceptor phase, ionic analytes may be preconcentrated considerably during LPME (typical preconcentration factors of 50 to 150). In addition, during LPME from biological samples such as plasma and urine, the majority of matrix components are not extracted into the acceptor phase resulting in excellent sample clean-up. For the extraction of basic drugs, dilute solutions of HCl or acidic phosphate buffers have been utilized as acceptor phase without further optimization. In the present work therefore, systematic studies have been performed to explore new acidic acceptor phase possibilities in combination with dihexyl ether as a standard organic solvent in the pores of the hollow fibre. In general, 10 mM solutions of HCl, H2SO4, and phosphate pH 3.3 were found to provide the highest extraction recoveries. pH was found to play a major role in adjusting the selectivity of LPME, whereas minor selectivity alterations were observed on changing the type of acid in the acceptor phase. For weakly basic drugs, an increased level of HCl in the acceptor phase may provide higher recovery, and for certain drugs, addition of ethanol to the acceptor phase may improve the extractability.

AB - Recently, we introduced an inexpensive and disposable hollow fibre based device for liquid-phase microextraction (LPME) where ionic analytes typically are extracted from 1-4 mL aqueous samples (such as plasma and urine) through an organic solvent immobilized in the pores of a polypropylene hollow fibre and into a 10-25 μL volume of acceptor phase present inside the hollow fibre. Because of the substantial volume ratio of the sample relative to the acceptor phase, ionic analytes may be preconcentrated considerably during LPME (typical preconcentration factors of 50 to 150). In addition, during LPME from biological samples such as plasma and urine, the majority of matrix components are not extracted into the acceptor phase resulting in excellent sample clean-up. For the extraction of basic drugs, dilute solutions of HCl or acidic phosphate buffers have been utilized as acceptor phase without further optimization. In the present work therefore, systematic studies have been performed to explore new acidic acceptor phase possibilities in combination with dihexyl ether as a standard organic solvent in the pores of the hollow fibre. In general, 10 mM solutions of HCl, H2SO4, and phosphate pH 3.3 were found to provide the highest extraction recoveries. pH was found to play a major role in adjusting the selectivity of LPME, whereas minor selectivity alterations were observed on changing the type of acid in the acceptor phase. For weakly basic drugs, an increased level of HCl in the acceptor phase may provide higher recovery, and for certain drugs, addition of ethanol to the acceptor phase may improve the extractability.

KW - 3-Phase extraction

KW - Basic drugs

KW - Liquid-phase microextraction (LPME)

KW - Porous hollow fibres

KW - Selectivity

UR - http://www.scopus.com/inward/record.url?scp=0036120728&partnerID=8YFLogxK

U2 - 10.1002/1615-9314(20020201)25:3<141::AID-JSSC141>3.0.CO;2-3

DO - 10.1002/1615-9314(20020201)25:3<141::AID-JSSC141>3.0.CO;2-3

M3 - Journal article

AN - SCOPUS:0036120728

VL - 25

SP - 141

EP - 146

JO - HRC &amp; CC, Journal of High Resolution Chromatography and Chromatography Communications

JF - HRC &amp; CC, Journal of High Resolution Chromatography and Chromatography Communications

SN - 1615-9306

IS - 3

ER -

ID: 231653134