Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Standard

Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device. / Feidenhans, Nikolaj A.; Jensen, Thomas Glasdam; Lafleur, Josiane P.; Kutter, Jörg P.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. p. 745-747.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Harvard

Feidenhans, NA, Jensen, TG, Lafleur, JP & Kutter, JP 2013, Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 2, Chemical and Biological Microsystems Society, pp. 745-747.

APA

Feidenhans, N. A., Jensen, T. G., Lafleur, J. P., & Kutter, J. P. (2013). Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 2, pp. 745-747). Chemical and Biological Microsystems Society.

Vancouver

Feidenhans NA, Jensen TG, Lafleur JP, Kutter JP. Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2. Chemical and Biological Microsystems Society. 2013. p. 745-747

Author

Feidenhans, Nikolaj A. ; Jensen, Thomas Glasdam ; Lafleur, Josiane P. ; Kutter, Jörg P. / Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 2 Chemical and Biological Microsystems Society, 2013. pp. 745-747

Bibtex

@inproceedings{fcfb34fcd7294913b164a4d62b7ed1b1,
title = "Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device",
abstract = "We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol-ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol-ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol-ene for optofluidic devices, the optical properties of thiol-ene was evaluated by determining the transparency and refractive index of the cured polymer.",
keywords = "Biomolecule immobilization, Optofluidic chip, Thiol-ene polymers",
author = "Feidenhans, {Nikolaj A.} and Jensen, {Thomas Glasdam} and Lafleur, {Josiane P.} and Kutter, {J{\"o}rg P.}",
year = "2013",
language = "English",
isbn = "9781632666246",
volume = "2",
pages = "745--747",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",

}

RIS

TY - GEN

T1 - Functionalization of embedded thiol-ene waveguides for evanescent wave-induced fluorescence detection in a microfluidic device

AU - Feidenhans, Nikolaj A.

AU - Jensen, Thomas Glasdam

AU - Lafleur, Josiane P.

AU - Kutter, Jörg P.

PY - 2013

Y1 - 2013

N2 - We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol-ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol-ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol-ene for optofluidic devices, the optical properties of thiol-ene was evaluated by determining the transparency and refractive index of the cured polymer.

AB - We demonstrate the use of functional surface groups inherently present on off-stoichiometric thiol-ene polymers, for site-specific immobilization of biomolecules and detection by evanescent wave-induced fluorescence. An optofluidic chip featuring an embedded thiol-ene waveguide was selectively functionalized with biotin using photografting. The biotin was used for immobilization of fluorescently labelled streptavidin, and experiments revealed a linear correlation between streptavidin concentration and fluorescent intensity. To further demonstrate the attractiveness of using thiol-ene for optofluidic devices, the optical properties of thiol-ene was evaluated by determining the transparency and refractive index of the cured polymer.

KW - Biomolecule immobilization

KW - Optofluidic chip

KW - Thiol-ene polymers

UR - http://www.scopus.com/inward/record.url?scp=84907330820&partnerID=8YFLogxK

M3 - Article in proceedings

AN - SCOPUS:84907330820

SN - 9781632666246

VL - 2

SP - 745

EP - 747

BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

PB - Chemical and Biological Microsystems Society

ER -

ID: 137375555