Functional nanoparticles exploit the bile acid pathway to overcome multiple barriers of the intestinal epithelium for oral insulin delivery

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Functional nanoparticles exploit the bile acid pathway to overcome multiple barriers of the intestinal epithelium for oral insulin delivery. / Fan, Weiwei; Xia, Dengning; Zhu, Quanlei; Li, Xiuying; He, Shufang; Zhu, Chunliu; Guo, Shiyan; Hovgaard, Lars; Yang, Mingshi; Gan, Yong.

In: Biomaterials, Vol. 151, 01.2018, p. 13-23.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Fan, W, Xia, D, Zhu, Q, Li, X, He, S, Zhu, C, Guo, S, Hovgaard, L, Yang, M & Gan, Y 2018, 'Functional nanoparticles exploit the bile acid pathway to overcome multiple barriers of the intestinal epithelium for oral insulin delivery', Biomaterials, vol. 151, pp. 13-23. https://doi.org/10.1016/j.biomaterials.2017.10.022

APA

Fan, W., Xia, D., Zhu, Q., Li, X., He, S., Zhu, C., Guo, S., Hovgaard, L., Yang, M., & Gan, Y. (2018). Functional nanoparticles exploit the bile acid pathway to overcome multiple barriers of the intestinal epithelium for oral insulin delivery. Biomaterials, 151, 13-23. https://doi.org/10.1016/j.biomaterials.2017.10.022

Vancouver

Fan W, Xia D, Zhu Q, Li X, He S, Zhu C et al. Functional nanoparticles exploit the bile acid pathway to overcome multiple barriers of the intestinal epithelium for oral insulin delivery. Biomaterials. 2018 Jan;151:13-23. https://doi.org/10.1016/j.biomaterials.2017.10.022

Author

Fan, Weiwei ; Xia, Dengning ; Zhu, Quanlei ; Li, Xiuying ; He, Shufang ; Zhu, Chunliu ; Guo, Shiyan ; Hovgaard, Lars ; Yang, Mingshi ; Gan, Yong. / Functional nanoparticles exploit the bile acid pathway to overcome multiple barriers of the intestinal epithelium for oral insulin delivery. In: Biomaterials. 2018 ; Vol. 151. pp. 13-23.

Bibtex

@article{8e8ab2c40d654cf3a3c09138a08cf765,

title = "Functional nanoparticles exploit the bile acid pathway to overcome multiple barriers of the intestinal epithelium for oral insulin delivery",

abstract = "Oral absorption of protein/peptide-loaded nanoparticles is often limited by multiple barriers of the intestinal epithelium. In addition to mucus translocation and apical endocytosis, highly efficient transepithelial absorption of nanoparticles requires successful intracellular trafficking, especially to avoid lysosomal degradation, and basolateral release. Here, the functional material, deoxycholic acid-conjugated chitosan, is synthesized and loaded with the model protein drug insulin into deoxycholic acid-modified nanoparticles (DNPs). The DNPs designed in this study are demonstrated to overcome multiple barriers of the intestinal epithelium by exploiting the bile acid pathway. In Caco-2 cell monolayers, DNPs are internalized via apical sodium-dependent bile acid transporter (ASBT)-mediated endocytosis. Interestingly, insulin degradation in the epithelium is significantly prevented due to endolysosomal escape of DNPs. Additionally, DNPs can interact with a cytosolic ileal bile acid-binding protein that facilitates the intracellular trafficking and basolateral release of insulin. In rats, intravital two-photon microscopy also reveals that the transport of DNPs into the intestinal villi is mediated by ASBT. Further pharmacokinetic studies disclose an oral bioavailability of 15.9% in type I diabetic rats after loading freeze-dried DNPs into enteric-coated capsules. Thus, deoxycholic acid-modified chitosan nanoparticles can overcome multiple barriers of the intestinal epithelium for oral delivery of insulin.",

keywords = "Journal Article",

author = "Weiwei Fan and Dengning Xia and Quanlei Zhu and Xiuying Li and Shufang He and Chunliu Zhu and Shiyan Guo and Lars Hovgaard and Mingshi Yang and Yong Gan",

year = "2018",

month = jan,

doi = "10.1016/j.biomaterials.2017.10.022",

language = "English",

volume = "151",

pages = "13--23",

journal = "Biomaterials",

issn = "0142-9612",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Functional nanoparticles exploit the bile acid pathway to overcome multiple barriers of the intestinal epithelium for oral insulin delivery

AU - Fan, Weiwei

AU - Xia, Dengning

AU - Zhu, Quanlei

AU - Li, Xiuying

AU - He, Shufang

AU - Zhu, Chunliu

AU - Guo, Shiyan

AU - Hovgaard, Lars

AU - Yang, Mingshi

AU - Gan, Yong

PY - 2018/1

Y1 - 2018/1

N2 - Oral absorption of protein/peptide-loaded nanoparticles is often limited by multiple barriers of the intestinal epithelium. In addition to mucus translocation and apical endocytosis, highly efficient transepithelial absorption of nanoparticles requires successful intracellular trafficking, especially to avoid lysosomal degradation, and basolateral release. Here, the functional material, deoxycholic acid-conjugated chitosan, is synthesized and loaded with the model protein drug insulin into deoxycholic acid-modified nanoparticles (DNPs). The DNPs designed in this study are demonstrated to overcome multiple barriers of the intestinal epithelium by exploiting the bile acid pathway. In Caco-2 cell monolayers, DNPs are internalized via apical sodium-dependent bile acid transporter (ASBT)-mediated endocytosis. Interestingly, insulin degradation in the epithelium is significantly prevented due to endolysosomal escape of DNPs. Additionally, DNPs can interact with a cytosolic ileal bile acid-binding protein that facilitates the intracellular trafficking and basolateral release of insulin. In rats, intravital two-photon microscopy also reveals that the transport of DNPs into the intestinal villi is mediated by ASBT. Further pharmacokinetic studies disclose an oral bioavailability of 15.9% in type I diabetic rats after loading freeze-dried DNPs into enteric-coated capsules. Thus, deoxycholic acid-modified chitosan nanoparticles can overcome multiple barriers of the intestinal epithelium for oral delivery of insulin.

AB - Oral absorption of protein/peptide-loaded nanoparticles is often limited by multiple barriers of the intestinal epithelium. In addition to mucus translocation and apical endocytosis, highly efficient transepithelial absorption of nanoparticles requires successful intracellular trafficking, especially to avoid lysosomal degradation, and basolateral release. Here, the functional material, deoxycholic acid-conjugated chitosan, is synthesized and loaded with the model protein drug insulin into deoxycholic acid-modified nanoparticles (DNPs). The DNPs designed in this study are demonstrated to overcome multiple barriers of the intestinal epithelium by exploiting the bile acid pathway. In Caco-2 cell monolayers, DNPs are internalized via apical sodium-dependent bile acid transporter (ASBT)-mediated endocytosis. Interestingly, insulin degradation in the epithelium is significantly prevented due to endolysosomal escape of DNPs. Additionally, DNPs can interact with a cytosolic ileal bile acid-binding protein that facilitates the intracellular trafficking and basolateral release of insulin. In rats, intravital two-photon microscopy also reveals that the transport of DNPs into the intestinal villi is mediated by ASBT. Further pharmacokinetic studies disclose an oral bioavailability of 15.9% in type I diabetic rats after loading freeze-dried DNPs into enteric-coated capsules. Thus, deoxycholic acid-modified chitosan nanoparticles can overcome multiple barriers of the intestinal epithelium for oral delivery of insulin.

KW - Journal Article

U2 - 10.1016/j.biomaterials.2017.10.022

DO - 10.1016/j.biomaterials.2017.10.022

M3 - Journal article

C2 - 29055774

VL - 151

SP - 13

EP - 23

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

ER -

ID: 185402866

Department of Pharmacy