Encapsulation into hyaluronic acid-based nanogels improves the selectivity index of the snake cathelicidin Ab-Cath

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Encapsulation into hyaluronic acid-based nanogels improves the selectivity index of the snake cathelicidin Ab-Cath. / van Gent, Miriam E.; Kłodzińska, Sylvia N.; Severin, Maureen; Ali, Muhanad; van Doodewaerd, Bjorn R.; Bos, Erik; Koning, Roman I.; Drijfhout, Jan Wouter; Nielsen, Hanne M.; Nibbering, Peter H.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 52, 102694, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

van Gent, ME, Kłodzińska, SN, Severin, M, Ali, M, van Doodewaerd, BR, Bos, E, Koning, RI, Drijfhout, JW, Nielsen, HM & Nibbering, PH 2023, 'Encapsulation into hyaluronic acid-based nanogels improves the selectivity index of the snake cathelicidin Ab-Cath', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 52, 102694. https://doi.org/10.1016/j.nano.2023.102694

APA

van Gent, M. E., Kłodzińska, S. N., Severin, M., Ali, M., van Doodewaerd, B. R., Bos, E., Koning, R. I., Drijfhout, J. W., Nielsen, H. M., & Nibbering, P. H. (2023). Encapsulation into hyaluronic acid-based nanogels improves the selectivity index of the snake cathelicidin Ab-Cath. Nanomedicine: Nanotechnology, Biology, and Medicine, 52, [102694]. https://doi.org/10.1016/j.nano.2023.102694

Vancouver

van Gent ME, Kłodzińska SN, Severin M, Ali M, van Doodewaerd BR, Bos E et al. Encapsulation into hyaluronic acid-based nanogels improves the selectivity index of the snake cathelicidin Ab-Cath. Nanomedicine: Nanotechnology, Biology, and Medicine. 2023;52. 102694. https://doi.org/10.1016/j.nano.2023.102694

Author

van Gent, Miriam E. ; Kłodzińska, Sylvia N. ; Severin, Maureen ; Ali, Muhanad ; van Doodewaerd, Bjorn R. ; Bos, Erik ; Koning, Roman I. ; Drijfhout, Jan Wouter ; Nielsen, Hanne M. ; Nibbering, Peter H. / Encapsulation into hyaluronic acid-based nanogels improves the selectivity index of the snake cathelicidin Ab-Cath. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2023 ; Vol. 52.

Bibtex

@article{4098f1e145b94438b56b32cdbf26eeef,
title = "Encapsulation into hyaluronic acid-based nanogels improves the selectivity index of the snake cathelicidin Ab-Cath",
abstract = "The antimicrobial peptide Ab-Cath, is a promising candidate for development as treatment for antimicrobial resistant (AMR) bacterial infections. Future clinical use is hampered by Ab-Cath's cationic peptidic nature and limited therapeutic window. Here, we evaluated hyaluronic acid-based nanogels for encapsulation of Ab-Cath to circumvent these limitations. Using microfluidics, monodispersed anionic nanogels of 156–232 nm encapsulating >99 % Ab-Cath were prepared. Unprecedented, lyophilization using polyvinyl alcohol and dextran-40 provided Ab-Cath nanogel protection and allowed easy dose adjustment. Lyophilized and redispersed Ab-Cath nanogels were as effective as Ab-Cath solution in killing AMR Staphylococcus aureus, Acinetobacter baumannii and Escherichia coli in biological fluids, and in reducing S. aureus and A. baumannii biofilms. Importantly, encapsulation of Ab-Cath in nanogels reduced Ab-Cath's cytotoxic effects on human fibroblasts by ≥10-fold. Moreover, cutaneous application of Ab-Cath nanogels eliminated bacteria colonizing 3D human skin. These findings affirm the use of nanogels to increase the selectivity index of antimicrobial peptides.",
keywords = "AMR infection, Antimicrobial peptide Ab-Cath, Drug delivery, Lyophilization method, Nanogel",
author = "{van Gent}, {Miriam E.} and K{\l}odzi{\'n}ska, {Sylvia N.} and Maureen Severin and Muhanad Ali and {van Doodewaerd}, {Bjorn R.} and Erik Bos and Koning, {Roman I.} and Drijfhout, {Jan Wouter} and Nielsen, {Hanne M.} and Nibbering, {Peter H.}",
note = "Funding Information: The authors acknowledge financial support from the Dutch Research Council (NWO), Novel Antibacterial Compounds and Therapies Antagonizing Resistance Program: grant number 16434 . S.N.K. and H.M.N. were supported by the Novo Nordisk Foundation , Grand Challenge Program: NNF16OC0021948 . ",
year = "2023",
doi = "10.1016/j.nano.2023.102694",
language = "English",
volume = "52",
journal = "Nanomedicine: Nanotechnology, Biology and Medicine",
issn = "1549-9634",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Encapsulation into hyaluronic acid-based nanogels improves the selectivity index of the snake cathelicidin Ab-Cath

AU - van Gent, Miriam E.

AU - Kłodzińska, Sylvia N.

AU - Severin, Maureen

AU - Ali, Muhanad

AU - van Doodewaerd, Bjorn R.

AU - Bos, Erik

AU - Koning, Roman I.

AU - Drijfhout, Jan Wouter

AU - Nielsen, Hanne M.

AU - Nibbering, Peter H.

N1 - Funding Information: The authors acknowledge financial support from the Dutch Research Council (NWO), Novel Antibacterial Compounds and Therapies Antagonizing Resistance Program: grant number 16434 . S.N.K. and H.M.N. were supported by the Novo Nordisk Foundation , Grand Challenge Program: NNF16OC0021948 .

PY - 2023

Y1 - 2023

N2 - The antimicrobial peptide Ab-Cath, is a promising candidate for development as treatment for antimicrobial resistant (AMR) bacterial infections. Future clinical use is hampered by Ab-Cath's cationic peptidic nature and limited therapeutic window. Here, we evaluated hyaluronic acid-based nanogels for encapsulation of Ab-Cath to circumvent these limitations. Using microfluidics, monodispersed anionic nanogels of 156–232 nm encapsulating >99 % Ab-Cath were prepared. Unprecedented, lyophilization using polyvinyl alcohol and dextran-40 provided Ab-Cath nanogel protection and allowed easy dose adjustment. Lyophilized and redispersed Ab-Cath nanogels were as effective as Ab-Cath solution in killing AMR Staphylococcus aureus, Acinetobacter baumannii and Escherichia coli in biological fluids, and in reducing S. aureus and A. baumannii biofilms. Importantly, encapsulation of Ab-Cath in nanogels reduced Ab-Cath's cytotoxic effects on human fibroblasts by ≥10-fold. Moreover, cutaneous application of Ab-Cath nanogels eliminated bacteria colonizing 3D human skin. These findings affirm the use of nanogels to increase the selectivity index of antimicrobial peptides.

AB - The antimicrobial peptide Ab-Cath, is a promising candidate for development as treatment for antimicrobial resistant (AMR) bacterial infections. Future clinical use is hampered by Ab-Cath's cationic peptidic nature and limited therapeutic window. Here, we evaluated hyaluronic acid-based nanogels for encapsulation of Ab-Cath to circumvent these limitations. Using microfluidics, monodispersed anionic nanogels of 156–232 nm encapsulating >99 % Ab-Cath were prepared. Unprecedented, lyophilization using polyvinyl alcohol and dextran-40 provided Ab-Cath nanogel protection and allowed easy dose adjustment. Lyophilized and redispersed Ab-Cath nanogels were as effective as Ab-Cath solution in killing AMR Staphylococcus aureus, Acinetobacter baumannii and Escherichia coli in biological fluids, and in reducing S. aureus and A. baumannii biofilms. Importantly, encapsulation of Ab-Cath in nanogels reduced Ab-Cath's cytotoxic effects on human fibroblasts by ≥10-fold. Moreover, cutaneous application of Ab-Cath nanogels eliminated bacteria colonizing 3D human skin. These findings affirm the use of nanogels to increase the selectivity index of antimicrobial peptides.

KW - AMR infection

KW - Antimicrobial peptide Ab-Cath

KW - Drug delivery

KW - Lyophilization method

KW - Nanogel

U2 - 10.1016/j.nano.2023.102694

DO - 10.1016/j.nano.2023.102694

M3 - Journal article

C2 - 37394107

AN - SCOPUS:85164280040

VL - 52

JO - Nanomedicine: Nanotechnology, Biology and Medicine

JF - Nanomedicine: Nanotechnology, Biology and Medicine

SN - 1549-9634

M1 - 102694

ER -

ID: 360246744