Elucidating the nanostructure of small interfering RNA-loaded lipidoid-polymer hybrid nanoparticles

Research output: Contribution to journal › Journal article › Research › peer-review

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Elucidating the nanostructure of small interfering RNA-loaded lipidoid-polymer hybrid nanoparticles. / Aljabbari, Anas; Lokras, Abhijeet Girish; Kirkensgaard, Jacob Judas Kain; Rades, Thomas; Franzyk, Henrik; Thakur, Aneesh; Zhang, Yibang; Foged, Camilla.

In: Journal of Colloid and Interface Science, Vol. 633, 2023, p. 907-922.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Aljabbari, A, Lokras, AG, Kirkensgaard, JJK, Rades, T, Franzyk, H, Thakur, A, Zhang, Y & Foged, C 2023, 'Elucidating the nanostructure of small interfering RNA-loaded lipidoid-polymer hybrid nanoparticles', Journal of Colloid and Interface Science, vol. 633, pp. 907-922. https://doi.org/10.1016/j.jcis.2022.11.141

APA

Aljabbari, A., Lokras, A. G., Kirkensgaard, J. J. K., Rades, T., Franzyk, H., Thakur, A., Zhang, Y., & Foged, C. (2023). Elucidating the nanostructure of small interfering RNA-loaded lipidoid-polymer hybrid nanoparticles. Journal of Colloid and Interface Science, 633, 907-922. https://doi.org/10.1016/j.jcis.2022.11.141

Vancouver

Aljabbari A, Lokras AG, Kirkensgaard JJK, Rades T, Franzyk H, Thakur A et al. Elucidating the nanostructure of small interfering RNA-loaded lipidoid-polymer hybrid nanoparticles. Journal of Colloid and Interface Science. 2023;633:907-922. https://doi.org/10.1016/j.jcis.2022.11.141

Author

Aljabbari, Anas ; Lokras, Abhijeet Girish ; Kirkensgaard, Jacob Judas Kain ; Rades, Thomas ; Franzyk, Henrik ; Thakur, Aneesh ; Zhang, Yibang ; Foged, Camilla. / Elucidating the nanostructure of small interfering RNA-loaded lipidoid-polymer hybrid nanoparticles. In: Journal of Colloid and Interface Science. 2023 ; Vol. 633. pp. 907-922.

Bibtex

@article{7d51c7017712451599ff626ca1268726,

title = "Elucidating the nanostructure of small interfering RNA-loaded lipidoid-polymer hybrid nanoparticles",

abstract = "We analyzed the structural and material properties of small interfering RNA (siRNA)-loaded lipid-polymer hybrid nanoparticles (LPNs) containing ionizable lipidoid and poly(dl-lactic-co-glycolic acid) (PLGA) using small-angle X-ray scattering, cryogenic transmission electron microscopy, polarized light microscopy, the Langmuir monolayer methodology, differential scanning calorimetry, and attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy. Scattering analyses showed that bulk lipidoid self-assemble into lamellar structures with a d-spacing of 38 {\AA}, whereas lipidoid-siRNA lipoplexes display an in-plane lateral organization of siRNA in between lipidoid bilayers with a repeat distance of approximately 55 {\AA}. The siRNA-loaded LPNs adopted a core-shell structure with an interaxial alignment of siRNA between lipidoid shell bilayers. Langmuir monolayer experiments showed a distinct interaction between the lipidoid headgroups and siRNA, which was dependent on buffer subphase pH. Thermal analyses suggested that PLGA and lipidoid interact, which was evident from a shift in the phase transition temperature of lipidoid, and the thermotropic phase behavior of lipidoid was affected by inclusion of siRNA. ATR-FTIR data confirmed the shift or disappearance of characteristic absorption bands of siRNA after lipidoid binding. In conclusion, siRNA-loaded LPNs display a core-shell structure, wherein the polymeric core functions as a colloid matrix support for siRNA-loaded lipidoid shell layers.",

author = "Anas Aljabbari and Lokras, {Abhijeet Girish} and Kirkensgaard, {Jacob Judas Kain} and Thomas Rades and Henrik Franzyk and Aneesh Thakur and Yibang Zhang and Camilla Foged",

year = "2023",

doi = "10.1016/j.jcis.2022.11.141",

language = "English",

volume = "633",

pages = "907--922",

journal = "Journal of Colloid and Interface Science",

issn = "0021-9797",

publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Elucidating the nanostructure of small interfering RNA-loaded lipidoid-polymer hybrid nanoparticles

AU - Aljabbari, Anas

AU - Lokras, Abhijeet Girish

AU - Kirkensgaard, Jacob Judas Kain

AU - Rades, Thomas

AU - Franzyk, Henrik

AU - Thakur, Aneesh

AU - Zhang, Yibang

AU - Foged, Camilla

PY - 2023

Y1 - 2023

N2 - We analyzed the structural and material properties of small interfering RNA (siRNA)-loaded lipid-polymer hybrid nanoparticles (LPNs) containing ionizable lipidoid and poly(dl-lactic-co-glycolic acid) (PLGA) using small-angle X-ray scattering, cryogenic transmission electron microscopy, polarized light microscopy, the Langmuir monolayer methodology, differential scanning calorimetry, and attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy. Scattering analyses showed that bulk lipidoid self-assemble into lamellar structures with a d-spacing of 38 Å, whereas lipidoid-siRNA lipoplexes display an in-plane lateral organization of siRNA in between lipidoid bilayers with a repeat distance of approximately 55 Å. The siRNA-loaded LPNs adopted a core-shell structure with an interaxial alignment of siRNA between lipidoid shell bilayers. Langmuir monolayer experiments showed a distinct interaction between the lipidoid headgroups and siRNA, which was dependent on buffer subphase pH. Thermal analyses suggested that PLGA and lipidoid interact, which was evident from a shift in the phase transition temperature of lipidoid, and the thermotropic phase behavior of lipidoid was affected by inclusion of siRNA. ATR-FTIR data confirmed the shift or disappearance of characteristic absorption bands of siRNA after lipidoid binding. In conclusion, siRNA-loaded LPNs display a core-shell structure, wherein the polymeric core functions as a colloid matrix support for siRNA-loaded lipidoid shell layers.

AB - We analyzed the structural and material properties of small interfering RNA (siRNA)-loaded lipid-polymer hybrid nanoparticles (LPNs) containing ionizable lipidoid and poly(dl-lactic-co-glycolic acid) (PLGA) using small-angle X-ray scattering, cryogenic transmission electron microscopy, polarized light microscopy, the Langmuir monolayer methodology, differential scanning calorimetry, and attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy. Scattering analyses showed that bulk lipidoid self-assemble into lamellar structures with a d-spacing of 38 Å, whereas lipidoid-siRNA lipoplexes display an in-plane lateral organization of siRNA in between lipidoid bilayers with a repeat distance of approximately 55 Å. The siRNA-loaded LPNs adopted a core-shell structure with an interaxial alignment of siRNA between lipidoid shell bilayers. Langmuir monolayer experiments showed a distinct interaction between the lipidoid headgroups and siRNA, which was dependent on buffer subphase pH. Thermal analyses suggested that PLGA and lipidoid interact, which was evident from a shift in the phase transition temperature of lipidoid, and the thermotropic phase behavior of lipidoid was affected by inclusion of siRNA. ATR-FTIR data confirmed the shift or disappearance of characteristic absorption bands of siRNA after lipidoid binding. In conclusion, siRNA-loaded LPNs display a core-shell structure, wherein the polymeric core functions as a colloid matrix support for siRNA-loaded lipidoid shell layers.

U2 - 10.1016/j.jcis.2022.11.141

DO - 10.1016/j.jcis.2022.11.141

M3 - Journal article

C2 - 36508398

VL - 633

SP - 907

EP - 922

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -

ID: 328424387

Department of Pharmacy