Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples

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Standard

Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples. / Vårdal, Linda; Gjelstad, Astrid; Huang, Chuixiu; Øiestad, Elisabeth Leere; Pedersen-Bjergaard, Stig.

In: Bioanalysis, Vol. 9, No. 8, 2017, p. 631-641.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Vårdal, L, Gjelstad, A, Huang, C, Øiestad, EL & Pedersen-Bjergaard, S 2017, 'Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples', Bioanalysis, vol. 9, no. 8, pp. 631-641. https://doi.org/10.4155/bio-2017-0027

APA

Vårdal, L., Gjelstad, A., Huang, C., Øiestad, E. L., & Pedersen-Bjergaard, S. (2017). Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples. Bioanalysis, 9(8), 631-641. https://doi.org/10.4155/bio-2017-0027

Vancouver

Vårdal L, Gjelstad A, Huang C, Øiestad EL, Pedersen-Bjergaard S. Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples. Bioanalysis. 2017;9(8):631-641. https://doi.org/10.4155/bio-2017-0027

Author

Vårdal, Linda ; Gjelstad, Astrid ; Huang, Chuixiu ; Øiestad, Elisabeth Leere ; Pedersen-Bjergaard, Stig. / Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples. In: Bioanalysis. 2017 ; Vol. 9, No. 8. pp. 631-641.

Bibtex

@article{19b1858f1d10423199320c81febed79e,
title = "Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples",
abstract = "AIM: For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Thhe purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems. RESULTS & DISCUSSION: No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts. CONCLUSION: Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.",
author = "Linda V{\aa}rdal and Astrid Gjelstad and Chuixiu Huang and {\O}iestad, {Elisabeth Leere} and Stig Pedersen-Bjergaard",
year = "2017",
doi = "10.4155/bio-2017-0027",
language = "English",
volume = "9",
pages = "631--641",
journal = "Bioanalysis",
issn = "1757-6180",
publisher = "Future Science",
number = "8",

}

RIS

TY - JOUR

T1 - Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples

AU - Vårdal, Linda

AU - Gjelstad, Astrid

AU - Huang, Chuixiu

AU - Øiestad, Elisabeth Leere

AU - Pedersen-Bjergaard, Stig

PY - 2017

Y1 - 2017

N2 - AIM: For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Thhe purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems. RESULTS & DISCUSSION: No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts. CONCLUSION: Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.

AB - AIM: For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Thhe purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems. RESULTS & DISCUSSION: No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts. CONCLUSION: Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.

U2 - 10.4155/bio-2017-0027

DO - 10.4155/bio-2017-0027

M3 - Journal article

C2 - 28508669

VL - 9

SP - 631

EP - 641

JO - Bioanalysis

JF - Bioanalysis

SN - 1757-6180

IS - 8

ER -

ID: 179140357