Effects of selective serotonin reuptake inhibitors on three sex steroids in two versions of the aromatase enzyme inhibition assay and in the H295R cell assay

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Effects of selective serotonin reuptake inhibitors on three sex steroids in two versions of the aromatase enzyme inhibition assay and in the H295R cell assay. / Jacobsen, Naja Wessel; Hansen, Cecilie Hurup; Nellemann, Christine; Styrishave, Bjarne; Halling-Sørensen, Bent.

In: Toxicology in Vitro, Vol. 29, No. 7, 10.2015, p. 1729-35.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jacobsen, NW, Hansen, CH, Nellemann, C, Styrishave, B & Halling-Sørensen, B 2015, 'Effects of selective serotonin reuptake inhibitors on three sex steroids in two versions of the aromatase enzyme inhibition assay and in the H295R cell assay', Toxicology in Vitro, vol. 29, no. 7, pp. 1729-35. https://doi.org/10.1016/j.tiv.2015.07.005

APA

Jacobsen, N. W., Hansen, C. H., Nellemann, C., Styrishave, B., & Halling-Sørensen, B. (2015). Effects of selective serotonin reuptake inhibitors on three sex steroids in two versions of the aromatase enzyme inhibition assay and in the H295R cell assay. Toxicology in Vitro, 29(7), 1729-35. https://doi.org/10.1016/j.tiv.2015.07.005

Vancouver

Jacobsen NW, Hansen CH, Nellemann C, Styrishave B, Halling-Sørensen B. Effects of selective serotonin reuptake inhibitors on three sex steroids in two versions of the aromatase enzyme inhibition assay and in the H295R cell assay. Toxicology in Vitro. 2015 Oct;29(7):1729-35. https://doi.org/10.1016/j.tiv.2015.07.005

Author

Jacobsen, Naja Wessel ; Hansen, Cecilie Hurup ; Nellemann, Christine ; Styrishave, Bjarne ; Halling-Sørensen, Bent. / Effects of selective serotonin reuptake inhibitors on three sex steroids in two versions of the aromatase enzyme inhibition assay and in the H295R cell assay. In: Toxicology in Vitro. 2015 ; Vol. 29, No. 7. pp. 1729-35.

Bibtex

@article{4cc3edd873c9435e807a5d8eefee9f64,

title = "Effects of selective serotonin reuptake inhibitors on three sex steroids in two versions of the aromatase enzyme inhibition assay and in the H295R cell assay",

abstract = "Selective serotonin reuptake inhibitors are known to have a range of disorders that are often linked to the endocrine system e.g. hormonal imbalances, breast enlargement, sexual dysfunction, and menstrual cycle disorders. The mechanisms behind most of these disorders are not known in details. In this study we investigated whether the endocrine effect due to SSRI exposure could be detected in well adopted in vitro steroidogenesis assays, two versions of the aromatase enzyme inhibition assay and the H295R cell assay. The five drugs citalopram, fluoxetine, fluvoxamine, paroxetine and sertraline, were shown to inhibit the aromatase enzyme in both types of aromatase assays. The IC50 values ranged from 3 to 600 μM. All five SSRIs, were further investigated in the H295R cell line. All compounds altered the steroid secretion from the cells, the lowest observed effect levels were 0.9 μM and 3.1 μM for sertraline and fluvoxamine, respectively. In general the H295R cell assay was more sensitive to SSRI exposure than the two aromatase assays, up to 20 times more sensitive. This indicates that the H295R cell line is a better tool for screening endocrine disrupting effects. Our findings show that the endocrine effects of SSRIs may, at least in part, be due to interference with the steroidogenesis.",

author = "Jacobsen, {Naja Wessel} and Hansen, {Cecilie Hurup} and Christine Nellemann and Bjarne Styrishave and Bent Halling-S{\o}rensen",

year = "2015",

month = oct,

doi = "10.1016/j.tiv.2015.07.005",

language = "English",

volume = "29",

pages = "1729--35",

journal = "Toxicology in Vitro",

issn = "0887-2333",

publisher = "Pergamon Press",

number = "7",

}

RIS

TY - JOUR

T1 - Effects of selective serotonin reuptake inhibitors on three sex steroids in two versions of the aromatase enzyme inhibition assay and in the H295R cell assay

AU - Jacobsen, Naja Wessel

AU - Hansen, Cecilie Hurup

AU - Nellemann, Christine

AU - Styrishave, Bjarne

AU - Halling-Sørensen, Bent

PY - 2015/10

Y1 - 2015/10

N2 - Selective serotonin reuptake inhibitors are known to have a range of disorders that are often linked to the endocrine system e.g. hormonal imbalances, breast enlargement, sexual dysfunction, and menstrual cycle disorders. The mechanisms behind most of these disorders are not known in details. In this study we investigated whether the endocrine effect due to SSRI exposure could be detected in well adopted in vitro steroidogenesis assays, two versions of the aromatase enzyme inhibition assay and the H295R cell assay. The five drugs citalopram, fluoxetine, fluvoxamine, paroxetine and sertraline, were shown to inhibit the aromatase enzyme in both types of aromatase assays. The IC50 values ranged from 3 to 600 μM. All five SSRIs, were further investigated in the H295R cell line. All compounds altered the steroid secretion from the cells, the lowest observed effect levels were 0.9 μM and 3.1 μM for sertraline and fluvoxamine, respectively. In general the H295R cell assay was more sensitive to SSRI exposure than the two aromatase assays, up to 20 times more sensitive. This indicates that the H295R cell line is a better tool for screening endocrine disrupting effects. Our findings show that the endocrine effects of SSRIs may, at least in part, be due to interference with the steroidogenesis.

AB - Selective serotonin reuptake inhibitors are known to have a range of disorders that are often linked to the endocrine system e.g. hormonal imbalances, breast enlargement, sexual dysfunction, and menstrual cycle disorders. The mechanisms behind most of these disorders are not known in details. In this study we investigated whether the endocrine effect due to SSRI exposure could be detected in well adopted in vitro steroidogenesis assays, two versions of the aromatase enzyme inhibition assay and the H295R cell assay. The five drugs citalopram, fluoxetine, fluvoxamine, paroxetine and sertraline, were shown to inhibit the aromatase enzyme in both types of aromatase assays. The IC50 values ranged from 3 to 600 μM. All five SSRIs, were further investigated in the H295R cell line. All compounds altered the steroid secretion from the cells, the lowest observed effect levels were 0.9 μM and 3.1 μM for sertraline and fluvoxamine, respectively. In general the H295R cell assay was more sensitive to SSRI exposure than the two aromatase assays, up to 20 times more sensitive. This indicates that the H295R cell line is a better tool for screening endocrine disrupting effects. Our findings show that the endocrine effects of SSRIs may, at least in part, be due to interference with the steroidogenesis.

U2 - 10.1016/j.tiv.2015.07.005

DO - 10.1016/j.tiv.2015.07.005

M3 - Journal article

C2 - 26162595

VL - 29

SP - 1729

EP - 1735

JO - Toxicology in Vitro

JF - Toxicology in Vitro

SN - 0887-2333

IS - 7

ER -

ID: 161623611

Department of Pharmacy