Effect of intra- and extra-liposomal distribution of sodium chloride on the stability of large unilamellar vesicles
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Effect of intra- and extra-liposomal distribution of sodium chloride on the stability of large unilamellar vesicles. / Siow, Lee Fong; Rades, Thomas; Lim, Miang Hoong.
In: Cryo-Letters, Vol. 28, No. 6, 2012, p. 429-44.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of intra- and extra-liposomal distribution of sodium chloride on the stability of large unilamellar vesicles
AU - Siow, Lee Fong
AU - Rades, Thomas
AU - Lim, Miang Hoong
PY - 2012
Y1 - 2012
N2 - Three groups of 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (DPPC) large unilamellar vesicle (LUV) dispersions were studied: LUV (A) dispersions with only extraliposomal sodium chloride (NaCl), LUV (B) dispersions with intra- and extraliposomal NaCl, and LUV (C) dispersions with only intraliposomal NaCl. The NaCl concentrations ranged from 0 to 150 mM. An abrupt increase in leakage was observed around -10 degree C for all the three groups of LUV, which coincided with the temperature of extraliposomal ice formation. Within the three groups, leakage of LUV (C) was significantly higher than the other groups. Extraliposomal ice formation and the resulting freeze-concentration of LUV may be the major cause of the leakage. Intraliposomal ice formation observed at -43 degree C seemed to stop leakage of LUV when LUV were frozen below -43 degree C. An exotherm of eutectic crystallization of NaCl was occasionally observed at -37 degree C, with a higher probability of formation at 150 mM extraliposomal NaCl than at 50 mM. The eutectic crystals were thought to cause additional leakage from the LUV (B).
AB - Three groups of 1,2-dipalmitoyl-rac-glycero-3-phosphocholine (DPPC) large unilamellar vesicle (LUV) dispersions were studied: LUV (A) dispersions with only extraliposomal sodium chloride (NaCl), LUV (B) dispersions with intra- and extraliposomal NaCl, and LUV (C) dispersions with only intraliposomal NaCl. The NaCl concentrations ranged from 0 to 150 mM. An abrupt increase in leakage was observed around -10 degree C for all the three groups of LUV, which coincided with the temperature of extraliposomal ice formation. Within the three groups, leakage of LUV (C) was significantly higher than the other groups. Extraliposomal ice formation and the resulting freeze-concentration of LUV may be the major cause of the leakage. Intraliposomal ice formation observed at -43 degree C seemed to stop leakage of LUV when LUV were frozen below -43 degree C. An exotherm of eutectic crystallization of NaCl was occasionally observed at -37 degree C, with a higher probability of formation at 150 mM extraliposomal NaCl than at 50 mM. The eutectic crystals were thought to cause additional leakage from the LUV (B).
M3 - Journal article
C2 - 18183324
VL - 28
SP - 429
EP - 444
JO - Cryo-Letters
JF - Cryo-Letters
SN - 0143-2044
IS - 6
ER -
ID: 40353936