Effect of incorporation of the adjuvant Quil A on structure and immune stimulatory capacity of liposomes
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Effect of incorporation of the adjuvant Quil A on structure and immune stimulatory capacity of liposomes. / Demana, Patrick H; Fehske, Christian; White, Karen; Rades, Thomas; Hook, Sarah.
In: Immunology and Cell Biology, Vol. 82, No. 5, 10.2004, p. 547-54.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Effect of incorporation of the adjuvant Quil A on structure and immune stimulatory capacity of liposomes
AU - Demana, Patrick H
AU - Fehske, Christian
AU - White, Karen
AU - Rades, Thomas
AU - Hook, Sarah
N1 - Copyright 2004 Australasian Society for Immunology Inc.
PY - 2004/10
Y1 - 2004/10
N2 - Liposomes have been widely used as drug delivery systems for many years. However, they are of limited use as delivery systems for subunit vaccines due to their low immunogenicity. Here we examine the effect of incorporating the adjuvant Quil A into liposomes on the type of particles produced, on the ability of the different particles to incorporate antigen and on the ability of the different particles to stimulate murine bone-marrow-derived dendritic cells (DC) and lymphocytes. The incorporation of increasing amounts of Quil A, from 20% to 70% of the total lipid into liposomes, reduces the size of the particles that form in aqueous dispersion and decreases antigen incorporation and uptake by DC. Interestingly, the particles with 20% Quil A were more toxic to cells in culture than the particles containing 70% Quil A, and the 20% particles were also more immunostimulatory.
AB - Liposomes have been widely used as drug delivery systems for many years. However, they are of limited use as delivery systems for subunit vaccines due to their low immunogenicity. Here we examine the effect of incorporating the adjuvant Quil A into liposomes on the type of particles produced, on the ability of the different particles to incorporate antigen and on the ability of the different particles to stimulate murine bone-marrow-derived dendritic cells (DC) and lymphocytes. The incorporation of increasing amounts of Quil A, from 20% to 70% of the total lipid into liposomes, reduces the size of the particles that form in aqueous dispersion and decreases antigen incorporation and uptake by DC. Interestingly, the particles with 20% Quil A were more toxic to cells in culture than the particles containing 70% Quil A, and the 20% particles were also more immunostimulatory.
KW - Adjuvants, Immunologic
KW - Animals
KW - Antigens
KW - Bone Marrow Cells
KW - Dendritic Cells
KW - Drug Delivery Systems
KW - Liposomes
KW - Lymphocyte Activation
KW - Mice
KW - Mice, Inbred Strains
KW - Particle Size
KW - Saponins
KW - T-Lymphocytes
U2 - 10.1111/j.0818-9641.2004.01276.x
DO - 10.1111/j.0818-9641.2004.01276.x
M3 - Journal article
C2 - 15479441
VL - 82
SP - 547
EP - 554
JO - Immunology and Cell Biology
JF - Immunology and Cell Biology
SN - 0818-9641
IS - 5
ER -
ID: 46408655