Easy peak tracking in CE-UV and CE-UV-ESI-MS by incorporating temperature-correlated mobility scaling
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Easy peak tracking in CE-UV and CE-UV-ESI-MS by incorporating temperature-correlated mobility scaling. / Li, Bin; Petersen, Nickolaj Jacob; Andersen, Line Hvass; Hansen, Steen Honoré.
In: Electrophoresis, Vol. 34, No. 12, 06.2013, p. 1787–1795.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Easy peak tracking in CE-UV and CE-UV-ESI-MS by incorporating temperature-correlated mobility scaling
AU - Li, Bin
AU - Petersen, Nickolaj Jacob
AU - Andersen, Line Hvass
AU - Hansen, Steen Honoré
N1 - © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
PY - 2013/6
Y1 - 2013/6
N2 - A simple data reconstruction technique in capillary electrophoresis - ultraviolet - electrospray ionization - mass spectrometry (CE-UV-ESI-MS) is presented to overcome the drift in mobilities caused by various factors compromising the reproducibility of such data, e.g. Joule heating effects and the variation in thermostatic control along the capillary, drift in electroosmotic flow (EOF) and the suction effect caused by the nebulizing gas in coaxial CE-MS interfaces. We present here a method to transform the traditional time-based electropherogram into the corresponding temperature-correlated mobility scale allowing tracking of analytes independent from capillary dimensions, electric field strengths, temperature control, and distance between the detectors. The main principle of this alignment is based on including the current in the mobility calculations and relating this to the initial electrical resistance of the buffer-filled capillary. The temperature-correlated mobility calculation eliminates the peak shifts due to the viscosity changes, improves the precision of peak identification using the observed temperature-correlated mobilities and allows a direct comparison of signals from different detection combinations. The method allows peaks from normal CE-UV separations to be correlated with the corresponding peak obtained by MS detection in CE-MS even for differences in capillary dimensions and thermostatic control.
AB - A simple data reconstruction technique in capillary electrophoresis - ultraviolet - electrospray ionization - mass spectrometry (CE-UV-ESI-MS) is presented to overcome the drift in mobilities caused by various factors compromising the reproducibility of such data, e.g. Joule heating effects and the variation in thermostatic control along the capillary, drift in electroosmotic flow (EOF) and the suction effect caused by the nebulizing gas in coaxial CE-MS interfaces. We present here a method to transform the traditional time-based electropherogram into the corresponding temperature-correlated mobility scale allowing tracking of analytes independent from capillary dimensions, electric field strengths, temperature control, and distance between the detectors. The main principle of this alignment is based on including the current in the mobility calculations and relating this to the initial electrical resistance of the buffer-filled capillary. The temperature-correlated mobility calculation eliminates the peak shifts due to the viscosity changes, improves the precision of peak identification using the observed temperature-correlated mobilities and allows a direct comparison of signals from different detection combinations. The method allows peaks from normal CE-UV separations to be correlated with the corresponding peak obtained by MS detection in CE-MS even for differences in capillary dimensions and thermostatic control.
U2 - 10.1002/elps.201200695
DO - 10.1002/elps.201200695
M3 - Journal article
C2 - 23576063
VL - 34
SP - 1787
EP - 1795
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 12
ER -
ID: 45391979