Distribution of terfenadine and its metabolites in locusts studied by desorption electrospray ionization mass spectrometry imaging
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Distribution of terfenadine and its metabolites in locusts studied by desorption electrospray ionization mass spectrometry imaging. / Olsen, Line Rørbæk; Hansen, Steen Honoré; Janfelt, Christian.
In: Analytical and Bioanalytical Chemistry, Vol. 407, No. 8, 2015, p. 2149–2158.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Distribution of terfenadine and its metabolites in locusts studied by desorption electrospray ionization mass spectrometry imaging
AU - Olsen, Line Rørbæk
AU - Hansen, Steen Honoré
AU - Janfelt, Christian
PY - 2015
Y1 - 2015
N2 - Desorption electrospray ionization (DESI) mass spectrometry (MS) imaging was used to image locusts dosed with the antihistamine drug terfenadine. The study was conducted in order to elucidate a relatively high elimination rate of terfenadine from the locust hemolymph. In this one of the few MS imaging studies on insects, a method for cryosectioning of whole locusts was developed, and the distributions of a number of endogenous compounds are reported, including betaine and a number of amino acids and phospholipids. Terfenadine was detected in the stomach region and the intestine walls, whereas three different metabolites—terfenadine acid (fexofenadine), terfenadine glucoside, and terfenadine phosphate—were detected in significantly smaller amounts and only in the unexcreted feces in the lower part of the intestine. The use of MS/MS imaging was necessary in order to detect the metabolites. With use of DESI-MS imaging, no colocalization of the drug and the metabolites was observed, suggesting a very rapid excretion of metabolites into the feces. Additional liquid chromatography–MS investigations were performed on hemolymph and feces and showed some abundance of terfenadine and the three metabolites, although at low levels, in both the hemolymph and the feces.
AB - Desorption electrospray ionization (DESI) mass spectrometry (MS) imaging was used to image locusts dosed with the antihistamine drug terfenadine. The study was conducted in order to elucidate a relatively high elimination rate of terfenadine from the locust hemolymph. In this one of the few MS imaging studies on insects, a method for cryosectioning of whole locusts was developed, and the distributions of a number of endogenous compounds are reported, including betaine and a number of amino acids and phospholipids. Terfenadine was detected in the stomach region and the intestine walls, whereas three different metabolites—terfenadine acid (fexofenadine), terfenadine glucoside, and terfenadine phosphate—were detected in significantly smaller amounts and only in the unexcreted feces in the lower part of the intestine. The use of MS/MS imaging was necessary in order to detect the metabolites. With use of DESI-MS imaging, no colocalization of the drug and the metabolites was observed, suggesting a very rapid excretion of metabolites into the feces. Additional liquid chromatography–MS investigations were performed on hemolymph and feces and showed some abundance of terfenadine and the three metabolites, although at low levels, in both the hemolymph and the feces.
U2 - 10.1007/s00216-014-8292-8
DO - 10.1007/s00216-014-8292-8
M3 - Journal article
C2 - 25404166
VL - 407
SP - 2149
EP - 2158
JO - Analytical and Bioanalytical Chemistry
JF - Analytical and Bioanalytical Chemistry
SN - 1618-2642
IS - 8
ER -
ID: 137379771