Direct Electromembrane Extraction‐Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids

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  • Frøydis Sved Skottvoll
  • Aleksandra Aizenshtadt
  • Frederik André Hansen
  • Mikel Amirola Martinez
  • Jannike Mørch Andersen
  • Inger Lise Bogen
  • Kutter, Jörg P.
  • Pedersen-Bjergaard, Stig
  • Elsa Lundanes
  • Stefan Krauss
  • Steven Ray Wilson
This work introduces a strategy for organoid analysis - direct Electromembrane Extraction based Mass Spectrometry (dEME-MS) – for coupling liver organoids with mass spectrometry (MS). dEME-MS comprises electrophoresis of selected small molecules from a culture chamber across an oil membrane, and to a MS compatible solution. This enables clean micro-extraction of drugs and their metabolites as produced in the liver organoids to capillary liquid chromatography-mass spectrometry. Applying dEME-MS, proof-of-concept of directly measuring methadone metabolism is demonstrated on adult liver organoids. With 50 liver organoids and 1 μM methadone, methadone metabolism was monitored from 0 to 24 hours (11 time points). All analytes had <0.4 % variance in retention times with >100 measurements. dEME-MS is capable of automated and selective monitoring of drug metabolism in liver organoids, and could serve as a valuable tool for automated drug discovery efforts.
Original languageEnglish
Article numbere202100051
JournalAnalysis & Sensing
Issue number2
Publication statusPublished - 2022

ID: 313016558