Development of a μDissolution-Permeation model with in situ drug concentration monitoring

Research output: Contribution to journalJournal articlepeer-review

Standard

Development of a μDissolution-Permeation model with in situ drug concentration monitoring. / Berthelsen, Ragna; Byrialsen, Julie Pelle; Holm, René; Jacobsen, Jette; Abrahamsson, Bertil; Saaby, Lasse; Madelung, Peter; Müllertz, Anette.

In: Journal of Drug Delivery Science and Technology, Vol. 35, 01.10.2016, p. 223-233.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Berthelsen, R, Byrialsen, JP, Holm, R, Jacobsen, J, Abrahamsson, B, Saaby, L, Madelung, P & Müllertz, A 2016, 'Development of a μDissolution-Permeation model with in situ drug concentration monitoring', Journal of Drug Delivery Science and Technology, vol. 35, pp. 223-233. https://doi.org/10.1016/j.jddst.2016.06.013

APA

Berthelsen, R., Byrialsen, J. P., Holm, R., Jacobsen, J., Abrahamsson, B., Saaby, L., Madelung, P., & Müllertz, A. (2016). Development of a μDissolution-Permeation model with in situ drug concentration monitoring. Journal of Drug Delivery Science and Technology, 35, 223-233. https://doi.org/10.1016/j.jddst.2016.06.013

Vancouver

Berthelsen R, Byrialsen JP, Holm R, Jacobsen J, Abrahamsson B, Saaby L et al. Development of a μDissolution-Permeation model with in situ drug concentration monitoring. Journal of Drug Delivery Science and Technology. 2016 Oct 1;35:223-233. https://doi.org/10.1016/j.jddst.2016.06.013

Author

Berthelsen, Ragna ; Byrialsen, Julie Pelle ; Holm, René ; Jacobsen, Jette ; Abrahamsson, Bertil ; Saaby, Lasse ; Madelung, Peter ; Müllertz, Anette. / Development of a μDissolution-Permeation model with in situ drug concentration monitoring. In: Journal of Drug Delivery Science and Technology. 2016 ; Vol. 35. pp. 223-233.

Bibtex

@article{e91089091a804f118568c7aee7ef36d0,
title = "Development of a μDissolution-Permeation model with in situ drug concentration monitoring",
abstract = "A μD/P model consisting of two connected compartments made of acrylic plastic was designed and constructed to fit in the μDiss Profiler{\texttrademark}. A Caco-2 cell monolayer grown on a Snapwell{\texttrademark} membrane insert was mounted between the apical and basolateral compartment serving as a permeability barrier. Fasted state biorelevant medium consisting of HBSS pH 6.5 supplemented with bile salts and lecithin was used as the apical dissolution media, while HBSS pH 7.4 was used as the basolateral medium. The apparent permeability (Papp) and dissolution-time profiles for albendazole, felodipine and fenofibrate were determined concomitantly using the μD/P model, and separately using a previously validated, common cell transwell permeability setup with Caco-2 cells as the permeability barrier, and the μDiss Profiler{\texttrademark}, respectively. The results were found to be in same range, when comparing data obtained using the different experimental setups.In conclusion, a μDiss compatible D/P model was developed enabling in situ measurement of apical drug concentrations and simultaneous determination of the amount of drug permeated across a Caco-2 cell monolayer.",
author = "Ragna Berthelsen and Byrialsen, {Julie Pelle} and Ren{\'e} Holm and Jette Jacobsen and Bertil Abrahamsson and Lasse Saaby and Peter Madelung and Anette M{\"u}llertz",
year = "2016",
month = oct,
day = "1",
doi = "10.1016/j.jddst.2016.06.013",
language = "English",
volume = "35",
pages = "223--233",
journal = "Journal of Drug Delivery Science and Technology",
issn = "1773-2247",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Development of a μDissolution-Permeation model with in situ drug concentration monitoring

AU - Berthelsen, Ragna

AU - Byrialsen, Julie Pelle

AU - Holm, René

AU - Jacobsen, Jette

AU - Abrahamsson, Bertil

AU - Saaby, Lasse

AU - Madelung, Peter

AU - Müllertz, Anette

PY - 2016/10/1

Y1 - 2016/10/1

N2 - A μD/P model consisting of two connected compartments made of acrylic plastic was designed and constructed to fit in the μDiss Profiler™. A Caco-2 cell monolayer grown on a Snapwell™ membrane insert was mounted between the apical and basolateral compartment serving as a permeability barrier. Fasted state biorelevant medium consisting of HBSS pH 6.5 supplemented with bile salts and lecithin was used as the apical dissolution media, while HBSS pH 7.4 was used as the basolateral medium. The apparent permeability (Papp) and dissolution-time profiles for albendazole, felodipine and fenofibrate were determined concomitantly using the μD/P model, and separately using a previously validated, common cell transwell permeability setup with Caco-2 cells as the permeability barrier, and the μDiss Profiler™, respectively. The results were found to be in same range, when comparing data obtained using the different experimental setups.In conclusion, a μDiss compatible D/P model was developed enabling in situ measurement of apical drug concentrations and simultaneous determination of the amount of drug permeated across a Caco-2 cell monolayer.

AB - A μD/P model consisting of two connected compartments made of acrylic plastic was designed and constructed to fit in the μDiss Profiler™. A Caco-2 cell monolayer grown on a Snapwell™ membrane insert was mounted between the apical and basolateral compartment serving as a permeability barrier. Fasted state biorelevant medium consisting of HBSS pH 6.5 supplemented with bile salts and lecithin was used as the apical dissolution media, while HBSS pH 7.4 was used as the basolateral medium. The apparent permeability (Papp) and dissolution-time profiles for albendazole, felodipine and fenofibrate were determined concomitantly using the μD/P model, and separately using a previously validated, common cell transwell permeability setup with Caco-2 cells as the permeability barrier, and the μDiss Profiler™, respectively. The results were found to be in same range, when comparing data obtained using the different experimental setups.In conclusion, a μDiss compatible D/P model was developed enabling in situ measurement of apical drug concentrations and simultaneous determination of the amount of drug permeated across a Caco-2 cell monolayer.

U2 - 10.1016/j.jddst.2016.06.013

DO - 10.1016/j.jddst.2016.06.013

M3 - Journal article

VL - 35

SP - 223

EP - 233

JO - Journal of Drug Delivery Science and Technology

JF - Journal of Drug Delivery Science and Technology

SN - 1773-2247

ER -

ID: 172887391