Determination of selenoprotein P in human plasma by solid phase extraction and inductively coupled plasma mass spectrometry
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Determination of selenoprotein P in human plasma by solid phase extraction and inductively coupled plasma mass spectrometry. / Bendahl, L.; Sidenius, U.; Gammelgaard, Bente.
In: Analytica Chimica Acta, Vol. 411, No. 1-2, 2000, p. 103-108.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Determination of selenoprotein P in human plasma by solid phase extraction and inductively coupled plasma mass spectrometry
AU - Bendahl, L.
AU - Sidenius, U.
AU - Gammelgaard, Bente
PY - 2000
Y1 - 2000
N2 - A method based on solid phase extraction was developed for the determination of selenoprotein P as selenium in human plasma. Separation of selenoprotein P from other selenium-containing proteins was accomplished by immobilized metal-ion affinity chromatography. The selenium content was subsequently measured by inductively coupled plasma mass spectrometry (ICP-MS) monitoring the Se-82 isotope. Linear response was observed in the concentration range 0.3-70.8 mu g/l selenium as selenoprotein P with a correlation coefficient of 0.9994. The precision expressed as relative standard deviation was better than 2% in this range. The estimated limit of detection was 2 mu g/l and the experimentally verified quantification limit was 5 mu g/l, giving a relative standard deviation less than 2%. (C) 2000 Elsevier Science B.V. All rights reserved
AB - A method based on solid phase extraction was developed for the determination of selenoprotein P as selenium in human plasma. Separation of selenoprotein P from other selenium-containing proteins was accomplished by immobilized metal-ion affinity chromatography. The selenium content was subsequently measured by inductively coupled plasma mass spectrometry (ICP-MS) monitoring the Se-82 isotope. Linear response was observed in the concentration range 0.3-70.8 mu g/l selenium as selenoprotein P with a correlation coefficient of 0.9994. The precision expressed as relative standard deviation was better than 2% in this range. The estimated limit of detection was 2 mu g/l and the experimentally verified quantification limit was 5 mu g/l, giving a relative standard deviation less than 2%. (C) 2000 Elsevier Science B.V. All rights reserved
M3 - Journal article
VL - 411
SP - 103
EP - 108
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 1-2
ER -
ID: 44288145