Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging. / D'Alvise, Janina; Mortensen, Rasmus; Hansen, Steen H; Janfelt, Christian.

In: Analytical and Bioanalytical Chemistry, Vol. 406, No. 15, 06.2014, p. 3735-42.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

D'Alvise, J, Mortensen, R, Hansen, SH & Janfelt, C 2014, 'Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging', Analytical and Bioanalytical Chemistry, vol. 406, no. 15, pp. 3735-42. https://doi.org/10.1007/s00216-014-7802-z

APA

D'Alvise, J., Mortensen, R., Hansen, S. H., & Janfelt, C. (2014). Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging. Analytical and Bioanalytical Chemistry, 406(15), 3735-42. https://doi.org/10.1007/s00216-014-7802-z

Vancouver

D'Alvise J, Mortensen R, Hansen SH, Janfelt C. Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging. Analytical and Bioanalytical Chemistry. 2014 Jun;406(15):3735-42. https://doi.org/10.1007/s00216-014-7802-z

Author

D'Alvise, Janina ; Mortensen, Rasmus ; Hansen, Steen H ; Janfelt, Christian. / Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging. In: Analytical and Bioanalytical Chemistry. 2014 ; Vol. 406, No. 15. pp. 3735-42.

Bibtex

@article{77c0a434449f41ba8537dc371273dffc,
title = "Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging",
abstract = "Desorption electrospray ionization (DESI) mass spectrometry imaging is demonstrated as a detection technique for penetration experiments of drugs in skin. Lidocaine ointment was used as the model compound in ex vivo experiments with whole pig ears as the skin model. Follicular transport of lidocaine into the deeper skin layers is demonstrated for the first time. Furthermore, metabolism of lidocaine to 3-OH-lidocaine was observed in subcutaneous tissue as well as in lobules of white adipose tissue surrounding the hair follicles. These results suggest that it is advantageous to use full thickness skin, including subcutaneous tissue, for skin metabolism studies.",
author = "Janina D'Alvise and Rasmus Mortensen and Hansen, {Steen H} and Christian Janfelt",
year = "2014",
month = jun,
doi = "10.1007/s00216-014-7802-z",
language = "English",
volume = "406",
pages = "3735--42",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer",
number = "15",

}

RIS

TY - JOUR

T1 - Detection of follicular transport of lidocaine and metabolism in adipose tissue in pig ear skin by DESI mass spectrometry imaging

AU - D'Alvise, Janina

AU - Mortensen, Rasmus

AU - Hansen, Steen H

AU - Janfelt, Christian

PY - 2014/6

Y1 - 2014/6

N2 - Desorption electrospray ionization (DESI) mass spectrometry imaging is demonstrated as a detection technique for penetration experiments of drugs in skin. Lidocaine ointment was used as the model compound in ex vivo experiments with whole pig ears as the skin model. Follicular transport of lidocaine into the deeper skin layers is demonstrated for the first time. Furthermore, metabolism of lidocaine to 3-OH-lidocaine was observed in subcutaneous tissue as well as in lobules of white adipose tissue surrounding the hair follicles. These results suggest that it is advantageous to use full thickness skin, including subcutaneous tissue, for skin metabolism studies.

AB - Desorption electrospray ionization (DESI) mass spectrometry imaging is demonstrated as a detection technique for penetration experiments of drugs in skin. Lidocaine ointment was used as the model compound in ex vivo experiments with whole pig ears as the skin model. Follicular transport of lidocaine into the deeper skin layers is demonstrated for the first time. Furthermore, metabolism of lidocaine to 3-OH-lidocaine was observed in subcutaneous tissue as well as in lobules of white adipose tissue surrounding the hair follicles. These results suggest that it is advantageous to use full thickness skin, including subcutaneous tissue, for skin metabolism studies.

U2 - 10.1007/s00216-014-7802-z

DO - 10.1007/s00216-014-7802-z

M3 - Journal article

C2 - 24722877

VL - 406

SP - 3735

EP - 3742

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 15

ER -

ID: 111310994