Design and fabrication of dual redox responsive nanoparticles with diselenide linkage combined photodynamically to effectively enhance gene expression
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Design and fabrication of dual redox responsive nanoparticles with diselenide linkage combined photodynamically to effectively enhance gene expression. / Fang, Yan; Lin, Xiaojie; Jin, Xuechao; Yang, Dongjuan; Gao, Shan; Shi, Kai; Yang, Mingshi.
In: International Journal of Nanomedicine, Vol. 15, 2020, p. 7297-7314.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Design and fabrication of dual redox responsive nanoparticles with diselenide linkage combined photodynamically to effectively enhance gene expression
AU - Fang, Yan
AU - Lin, Xiaojie
AU - Jin, Xuechao
AU - Yang, Dongjuan
AU - Gao, Shan
AU - Shi, Kai
AU - Yang, Mingshi
PY - 2020
Y1 - 2020
N2 - Background: PEI is currently the most used non-viral gene carrier and the transfection efficiency is closely related to the molecular weight; however, the prominent problem is that the cytotoxicity increased with the molecular weight. Methods: A novel redox responsive biodegradable diselenide cross-linked polymer (dPSP) was designed to enhance gene expression. ICG-pEGFP-TRAIL/dPSP nanoparticles with high drug loading are prepared, which have redox sensitivity and plasmid protection. The trans-fection efficiency of dPSP nanoparticle was evaluated in vitro. Results: The plasmid was compressed by 100% at the N/P ratio of 16, and the particle size was less than 100 nm. When explored onto high concentrations of GSH/H2O2, dPSP4 degraded into small molecular weight cationic substances with low cytotoxicity rapidly. Singlet oxygen (1O2) was produced when indocyanine green (ICG) was irradiated by near-infrared laser irradiation (NIR) to promote oxidative degradation of dPSP4 nanoparticles. Under the stimulation of NIR 808 and redox agent, the particle size and PDI of ICG-pDNA /dPSP nanoparticle increased significantly. Conclusion: Compared with gene therapy alone, co-transportation of dPSP4 nanoparticle with ICG and pEGFP-TRAIL had better antitumor effect. Diselenide-crosslinked polysper-mine had a promising prospect on gene delivery and preparation of multifunctional anti-tumor carrier.
AB - Background: PEI is currently the most used non-viral gene carrier and the transfection efficiency is closely related to the molecular weight; however, the prominent problem is that the cytotoxicity increased with the molecular weight. Methods: A novel redox responsive biodegradable diselenide cross-linked polymer (dPSP) was designed to enhance gene expression. ICG-pEGFP-TRAIL/dPSP nanoparticles with high drug loading are prepared, which have redox sensitivity and plasmid protection. The trans-fection efficiency of dPSP nanoparticle was evaluated in vitro. Results: The plasmid was compressed by 100% at the N/P ratio of 16, and the particle size was less than 100 nm. When explored onto high concentrations of GSH/H2O2, dPSP4 degraded into small molecular weight cationic substances with low cytotoxicity rapidly. Singlet oxygen (1O2) was produced when indocyanine green (ICG) was irradiated by near-infrared laser irradiation (NIR) to promote oxidative degradation of dPSP4 nanoparticles. Under the stimulation of NIR 808 and redox agent, the particle size and PDI of ICG-pDNA /dPSP nanoparticle increased significantly. Conclusion: Compared with gene therapy alone, co-transportation of dPSP4 nanoparticle with ICG and pEGFP-TRAIL had better antitumor effect. Diselenide-crosslinked polysper-mine had a promising prospect on gene delivery and preparation of multifunctional anti-tumor carrier.
KW - Biodegradable
KW - Diselenide
KW - Gene delivery
KW - Polyspermine
KW - Single-line oxygen
U2 - 10.2147/IJN.S266514
DO - 10.2147/IJN.S266514
M3 - Journal article
C2 - 33061382
AN - SCOPUS:85092135657
VL - 15
SP - 7297
EP - 7314
JO - International Journal of Nanomedicine
JF - International Journal of Nanomedicine
SN - 1176-9114
ER -
ID: 250376972