Data demonstrating the challenges of determining the kinetic parameters of P-gp mediated transport of low-water soluble substrates

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Data demonstrating the challenges of determining the kinetic parameters of P-gp mediated transport of low-water soluble substrates. / Ozgür, Burak; Saaby, Lasse; Langthaler, Kristine; Brodin, Birger.

In: Data in Brief, Vol. 16, 2018, p. 655-659.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Ozgür, B, Saaby, L, Langthaler, K & Brodin, B 2018, 'Data demonstrating the challenges of determining the kinetic parameters of P-gp mediated transport of low-water soluble substrates', Data in Brief, vol. 16, pp. 655-659. https://doi.org/10.1016/j.dib.2017.11.092

APA

Ozgür, B., Saaby, L., Langthaler, K., & Brodin, B. (2018). Data demonstrating the challenges of determining the kinetic parameters of P-gp mediated transport of low-water soluble substrates. Data in Brief, 16, 655-659. https://doi.org/10.1016/j.dib.2017.11.092

Vancouver

Ozgür B, Saaby L, Langthaler K, Brodin B. Data demonstrating the challenges of determining the kinetic parameters of P-gp mediated transport of low-water soluble substrates. Data in Brief. 2018;16:655-659. https://doi.org/10.1016/j.dib.2017.11.092

Author

Ozgür, Burak ; Saaby, Lasse ; Langthaler, Kristine ; Brodin, Birger. / Data demonstrating the challenges of determining the kinetic parameters of P-gp mediated transport of low-water soluble substrates. In: Data in Brief. 2018 ; Vol. 16. pp. 655-659.

Bibtex

@article{262c5a7f61fc48eea35da67990c46955,
title = "Data demonstrating the challenges of determining the kinetic parameters of P-gp mediated transport of low-water soluble substrates",
abstract = "The presented data are related to the research article entitled “Characterization of the IPEC-J2 MDR1 (iP-gp) cell line as a tool for identification of P-gp substrates” by Ozgur et al. (2017). This data report describes the challenges of investigating the concentration-dependent transport of P- glycoprotein (P-gp) substrates with relatively low aqueous solubility. Thus, we provide solubility data on two prototypical P-gp substrates, digoxin and rhodamine 123, representing P-gp substrates with a relatively low and high-aqueous solubility, respectively. We present a hypothetical Michaelis-Menten curve of the P-gp mediated transport of digoxin to demonstrate that the maximal donor concentration, which can be reached in the experimental transport buffer, is too low to yield transport data in the saturable range of the MichaelisMenten relationship. Furthermore, we present data on the bidirectional transport of digoxin and rhodamine 123 across cell monolayers of the MDCK II MDR1 cell line and iP-pg cell line in the presence of the selective P-gp inhibitor, zosuquidar/LY335979.",
author = "Burak Ozg{\"u}r and Lasse Saaby and Kristine Langthaler and Birger Brodin",
year = "2018",
doi = "10.1016/j.dib.2017.11.092",
language = "English",
volume = "16",
pages = "655--659",
journal = "Data in Brief",
issn = "2352-3409",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Data demonstrating the challenges of determining the kinetic parameters of P-gp mediated transport of low-water soluble substrates

AU - Ozgür, Burak

AU - Saaby, Lasse

AU - Langthaler, Kristine

AU - Brodin, Birger

PY - 2018

Y1 - 2018

N2 - The presented data are related to the research article entitled “Characterization of the IPEC-J2 MDR1 (iP-gp) cell line as a tool for identification of P-gp substrates” by Ozgur et al. (2017). This data report describes the challenges of investigating the concentration-dependent transport of P- glycoprotein (P-gp) substrates with relatively low aqueous solubility. Thus, we provide solubility data on two prototypical P-gp substrates, digoxin and rhodamine 123, representing P-gp substrates with a relatively low and high-aqueous solubility, respectively. We present a hypothetical Michaelis-Menten curve of the P-gp mediated transport of digoxin to demonstrate that the maximal donor concentration, which can be reached in the experimental transport buffer, is too low to yield transport data in the saturable range of the MichaelisMenten relationship. Furthermore, we present data on the bidirectional transport of digoxin and rhodamine 123 across cell monolayers of the MDCK II MDR1 cell line and iP-pg cell line in the presence of the selective P-gp inhibitor, zosuquidar/LY335979.

AB - The presented data are related to the research article entitled “Characterization of the IPEC-J2 MDR1 (iP-gp) cell line as a tool for identification of P-gp substrates” by Ozgur et al. (2017). This data report describes the challenges of investigating the concentration-dependent transport of P- glycoprotein (P-gp) substrates with relatively low aqueous solubility. Thus, we provide solubility data on two prototypical P-gp substrates, digoxin and rhodamine 123, representing P-gp substrates with a relatively low and high-aqueous solubility, respectively. We present a hypothetical Michaelis-Menten curve of the P-gp mediated transport of digoxin to demonstrate that the maximal donor concentration, which can be reached in the experimental transport buffer, is too low to yield transport data in the saturable range of the MichaelisMenten relationship. Furthermore, we present data on the bidirectional transport of digoxin and rhodamine 123 across cell monolayers of the MDCK II MDR1 cell line and iP-pg cell line in the presence of the selective P-gp inhibitor, zosuquidar/LY335979.

U2 - 10.1016/j.dib.2017.11.092

DO - 10.1016/j.dib.2017.11.092

M3 - Journal article

C2 - 29541662

VL - 16

SP - 655

EP - 659

JO - Data in Brief

JF - Data in Brief

SN - 2352-3409

ER -

ID: 195227058