Correlation between calculated molecular descriptors of excipient amino acids and experimentally observed thermal stability of lysozyme

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Helena Meng-Lund, Natascha Friis, Marco van de Weert, Jukka Rantanen, Antti Poso, Holger Grohganz, Lene Jorgensen

A quantitative structure-property relationship (QSPR) between protein stability and the physicochemical properties of excipients was investigated to enable a more rational choice of stabilizing excipients than prior knowledge. The thermal transition temperature and aggregation time were determined for lysozyme in combination with 13 different amino acids using high throughput fluorescence spectroscopy and kinetic static light scattering measurements. On the theoretical side, around 200 2D and 3D molecular descriptors were calculated based on the amino acids' chemical structure. Multivariate data analysis was applied to correlate the descriptors with the experimental results. It was possible to identify descriptors, i.e. amino acids properties, with a positive influence on either transition temperature or aggregation onset time, or both. A high number of hydrogen bond acceptor moieties was the most prominent stabilizing factor for both responses, whereas hydrophilic surface properties and high molecular mass density mostly had a positive influence on the unfolding temperature. A high partition coefficient (logP(o/w)) was identified as the most prominent destabilizing factor for both responses. The QSPR shows good correlation between calculated molecular descriptors and the measured stabilizing effect of amino acids on lysozyme.

Original languageEnglish
JournalInternational Journal of Pharmaceutics
Issue number1
Pages (from-to)238-245
Number of pages8
Publication statusPublished - 15 May 2017

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  • Journal Article

ID: 176884291