Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces

Research output: Contribution to journalJournal articlepeer-review

Standard

Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces. / Kapp, Sebastian J; Larsson, Iben; van de Weert, Marco; Cardenas Gomez, Marite; Jorgensen, Lene.

In: Journal of Pharmaceutical Sciences, Vol. 104, No. 2, 2015, p. 593-601.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Kapp, SJ, Larsson, I, van de Weert, M, Cardenas Gomez, M & Jorgensen, L 2015, 'Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces', Journal of Pharmaceutical Sciences, vol. 104, no. 2, pp. 593-601. https://doi.org/10.1002/jps.24265

APA

Kapp, S. J., Larsson, I., van de Weert, M., Cardenas Gomez, M., & Jorgensen, L. (2015). Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces. Journal of Pharmaceutical Sciences, 104(2), 593-601. https://doi.org/10.1002/jps.24265

Vancouver

Kapp SJ, Larsson I, van de Weert M, Cardenas Gomez M, Jorgensen L. Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces. Journal of Pharmaceutical Sciences. 2015;104(2):593-601. https://doi.org/10.1002/jps.24265

Author

Kapp, Sebastian J ; Larsson, Iben ; van de Weert, Marco ; Cardenas Gomez, Marite ; Jorgensen, Lene. / Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces. In: Journal of Pharmaceutical Sciences. 2015 ; Vol. 104, No. 2. pp. 593-601.

Bibtex

@article{ec5d2f07f0af4fceb78941b9d83793f7,
title = "Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces",
abstract = "Two monoclonal antibodies from the IgG subclasses one and two were compared in their adsorption behavior with hydrophobic surfaces upon dilution to 10 mg/mL with 0.9% NaCl. These conditions simulate handling of the compounds at hospital pharmacies and surfaces encountered after preparation, such as infusion bags and i.v. lines. Total internal reflection fluorescence and quartz crystal microbalance with dissipation monitoring were used to follow and quantify this. Furthermore, the influence of the nonionic surfactant polysorbate 80 (PS80) on the adsorption process of these two antibodies was investigated. Despite belonging to two different IgG subclasses, both antibodies displayed comparable adsorption behavior. Both antibodies readily adsorbed in the absence of PS80, whereas adsorption was reduced in the presence of 30 mg/L surfactant. The sequence of exposure of the surfactant and protein to the surface was found to have a major influence on the extent of protein adsorption. Although only a fraction of adsorbed protein could be removed by rinsing with 30 mg/L surfactant solution, adsorption was entirely prevented when surfaces were pre-exposed to PS80. {\textcopyright} 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci.",
author = "Kapp, {Sebastian J} and Iben Larsson and {van de Weert}, Marco and {Cardenas Gomez}, Marite and Lene Jorgensen",
note = "{\textcopyright} 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.",
year = "2015",
doi = "10.1002/jps.24265",
language = "English",
volume = "104",
pages = "593--601",
journal = "Journal of Pharmaceutical Sciences",
issn = "0022-3549",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Competitive adsorption of monoclonal antibodies and nonionic surfactants at solid hydrophobic surfaces

AU - Kapp, Sebastian J

AU - Larsson, Iben

AU - van de Weert, Marco

AU - Cardenas Gomez, Marite

AU - Jorgensen, Lene

N1 - © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association.

PY - 2015

Y1 - 2015

N2 - Two monoclonal antibodies from the IgG subclasses one and two were compared in their adsorption behavior with hydrophobic surfaces upon dilution to 10 mg/mL with 0.9% NaCl. These conditions simulate handling of the compounds at hospital pharmacies and surfaces encountered after preparation, such as infusion bags and i.v. lines. Total internal reflection fluorescence and quartz crystal microbalance with dissipation monitoring were used to follow and quantify this. Furthermore, the influence of the nonionic surfactant polysorbate 80 (PS80) on the adsorption process of these two antibodies was investigated. Despite belonging to two different IgG subclasses, both antibodies displayed comparable adsorption behavior. Both antibodies readily adsorbed in the absence of PS80, whereas adsorption was reduced in the presence of 30 mg/L surfactant. The sequence of exposure of the surfactant and protein to the surface was found to have a major influence on the extent of protein adsorption. Although only a fraction of adsorbed protein could be removed by rinsing with 30 mg/L surfactant solution, adsorption was entirely prevented when surfaces were pre-exposed to PS80. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci.

AB - Two monoclonal antibodies from the IgG subclasses one and two were compared in their adsorption behavior with hydrophobic surfaces upon dilution to 10 mg/mL with 0.9% NaCl. These conditions simulate handling of the compounds at hospital pharmacies and surfaces encountered after preparation, such as infusion bags and i.v. lines. Total internal reflection fluorescence and quartz crystal microbalance with dissipation monitoring were used to follow and quantify this. Furthermore, the influence of the nonionic surfactant polysorbate 80 (PS80) on the adsorption process of these two antibodies was investigated. Despite belonging to two different IgG subclasses, both antibodies displayed comparable adsorption behavior. Both antibodies readily adsorbed in the absence of PS80, whereas adsorption was reduced in the presence of 30 mg/L surfactant. The sequence of exposure of the surfactant and protein to the surface was found to have a major influence on the extent of protein adsorption. Although only a fraction of adsorbed protein could be removed by rinsing with 30 mg/L surfactant solution, adsorption was entirely prevented when surfaces were pre-exposed to PS80. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci.

U2 - 10.1002/jps.24265

DO - 10.1002/jps.24265

M3 - Journal article

C2 - 25446557

VL - 104

SP - 593

EP - 601

JO - Journal of Pharmaceutical Sciences

JF - Journal of Pharmaceutical Sciences

SN - 0022-3549

IS - 2

ER -

ID: 128688365