Chromatographic methods in the monitoring of lipase-catalyzed interesterification

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Chromatographic methods in the monitoring of lipase-catalyzed interesterification. / Mu, Huiling; Kalo, P.; Xu, X.; Høy, Carl-Erik.

In: European Journal of Lipid Science and Technology, Vol. 102, No. 3, 01.01.2000, p. 202-211.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mu, H, Kalo, P, Xu, X & Høy, C-E 2000, 'Chromatographic methods in the monitoring of lipase-catalyzed interesterification', European Journal of Lipid Science and Technology, vol. 102, no. 3, pp. 202-211.

APA

Mu, H., Kalo, P., Xu, X., & Høy, C-E. (2000). Chromatographic methods in the monitoring of lipase-catalyzed interesterification. European Journal of Lipid Science and Technology, 102(3), 202-211.

Vancouver

Mu H, Kalo P, Xu X, Høy C-E. Chromatographic methods in the monitoring of lipase-catalyzed interesterification. European Journal of Lipid Science and Technology. 2000 Jan 1;102(3):202-211.

Author

Mu, Huiling ; Kalo, P. ; Xu, X. ; Høy, Carl-Erik. / Chromatographic methods in the monitoring of lipase-catalyzed interesterification. In: European Journal of Lipid Science and Technology. 2000 ; Vol. 102, No. 3. pp. 202-211.

Bibtex

@article{d9508af13c434912a71a6a5b820aef77,
title = "Chromatographic methods in the monitoring of lipase-catalyzed interesterification",
abstract = "Structured lipids containing special fatty acids were produced via lipase-catalyzed interesterification. Chromatographic methods were modified for the analysis of the acylglycerol content of the selected interesterified products, resulting from different reacting substrate mixtures and reaction systems. A modified normal-phase high performance liquid chromatographic method with evaporative light-scattering detection was used in the analysis of the diacylglycerols formed as intermediate products during interesterification. One of the advantages of the method was the low solvent consumption through the use of a narrow-bore silica column. Another advantage was in the speed of the analysis. Using a suitable solvent gradient, both diacylglycerol and triacylglycerol components were separated by reversed-phase high performance liquid chromatography within 50 min, providing both the diacylglycerol content and the triacylglycerol composition. High-temperature gas chromatography was also used to separate both diacylglycerol and triacylglycerol molecular species after trimethylsilylation of the structured lipid samples. Good agreements in the values obtained for the concentrations of diacylglycerols and the triacylglycerol compositions were apparent with the different chromatographic methods.",
author = "Huiling Mu and P. Kalo and X. Xu and Carl-Erik H{\o}y",
year = "2000",
month = jan,
day = "1",
language = "English",
volume = "102",
pages = "202--211",
journal = "European Journal of Lipid Science and Technology",
issn = "1438-7697",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "3",

}

RIS

TY - JOUR

T1 - Chromatographic methods in the monitoring of lipase-catalyzed interesterification

AU - Mu, Huiling

AU - Kalo, P.

AU - Xu, X.

AU - Høy, Carl-Erik

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Structured lipids containing special fatty acids were produced via lipase-catalyzed interesterification. Chromatographic methods were modified for the analysis of the acylglycerol content of the selected interesterified products, resulting from different reacting substrate mixtures and reaction systems. A modified normal-phase high performance liquid chromatographic method with evaporative light-scattering detection was used in the analysis of the diacylglycerols formed as intermediate products during interesterification. One of the advantages of the method was the low solvent consumption through the use of a narrow-bore silica column. Another advantage was in the speed of the analysis. Using a suitable solvent gradient, both diacylglycerol and triacylglycerol components were separated by reversed-phase high performance liquid chromatography within 50 min, providing both the diacylglycerol content and the triacylglycerol composition. High-temperature gas chromatography was also used to separate both diacylglycerol and triacylglycerol molecular species after trimethylsilylation of the structured lipid samples. Good agreements in the values obtained for the concentrations of diacylglycerols and the triacylglycerol compositions were apparent with the different chromatographic methods.

AB - Structured lipids containing special fatty acids were produced via lipase-catalyzed interesterification. Chromatographic methods were modified for the analysis of the acylglycerol content of the selected interesterified products, resulting from different reacting substrate mixtures and reaction systems. A modified normal-phase high performance liquid chromatographic method with evaporative light-scattering detection was used in the analysis of the diacylglycerols formed as intermediate products during interesterification. One of the advantages of the method was the low solvent consumption through the use of a narrow-bore silica column. Another advantage was in the speed of the analysis. Using a suitable solvent gradient, both diacylglycerol and triacylglycerol components were separated by reversed-phase high performance liquid chromatography within 50 min, providing both the diacylglycerol content and the triacylglycerol composition. High-temperature gas chromatography was also used to separate both diacylglycerol and triacylglycerol molecular species after trimethylsilylation of the structured lipid samples. Good agreements in the values obtained for the concentrations of diacylglycerols and the triacylglycerol compositions were apparent with the different chromatographic methods.

UR - http://www.scopus.com/inward/record.url?scp=0034363474&partnerID=8YFLogxK

M3 - Journal article

AN - SCOPUS:0034363474

VL - 102

SP - 202

EP - 211

JO - European Journal of Lipid Science and Technology

JF - European Journal of Lipid Science and Technology

SN - 1438-7697

IS - 3

ER -

ID: 45572806