Chitosan hydrogels containing liposomes and cubosomes as particulate sustained release vaccine delivery systems

Research output: Contribution to journalJournal articleResearchpeer-review

  • Sarah Gordon
  • Katherine Young
  • Rachel Wilson
  • Shakila Rizwan
  • Roslyn Kemp
  • Rades, Thomas
  • Sarah Hook

Sustained release depot systems have been widely investigated for their potential to improve the efficacy of subunit vaccines and reduce the requirement for boosting. The present study aimed to further enhance the immunogenicity of a sustained release vaccine by combining a depot formulation with a particulate antigen delivery system. Sustained release of the model subunit antigen, ovalbumin (OVA), was observed in vivo from chitosan thermogel-based formulations containing cationic, nanosized liposomes loaded with OVA and the immunopotentiator, Quil A (QA). Such formulations demonstrated the ability to induce cluster of differentiation (CD)8+ and CD4+ T-cell proliferation and interferon (IFN)-γ production, as well as the production of OVA-specific antibody. However, gel-incorporated liposomes showed evidence of instability and similar in vivo immune responses to liposomes in gel formulations were induced by gel-based systems loaded with soluble OVA and QA. The immunogenicity of chitosan thermogels containing cubosomes, a more stable lipidic particulate system, was therefore examined. Similarly, all gel-based formulations produced comparable effector immune responses in experimental mice, irrespective of whether the antigen and immunopotentiator were present in gels within cubosomes or in a soluble form. This work demonstrates the potential for sustained release thermogelling systems and highlights the importance of matching the physicochemical and immunological properties of the particulate system to that of the depot.

Original languageEnglish
JournalJournal of Liposome Research
Issue number3
Pages (from-to)193-204
Number of pages12
Publication statusPublished - Sep 2012

Bibliographical note

Funding Information:
This research was financially supported by the University of Otago. S.G. gratefully acknowledges the University of Otago and the National School of Pharmacy for the provision of a University of Otago Postgraduate Scholarship and a Ph.D. stipend, respectively.

    Research areas

  • Immune response, Particulate delivery systems, Sustained release delivery systems, Thermosensitive hydrogel

ID: 299415718