Ancient forest plants possess cytotoxic properties causing liver cancer HepG2 cell apoptosis
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Ancient forest plants possess cytotoxic properties causing liver cancer HepG2 cell apoptosis. / Yue, Xiaochen; Ling Ma, Nyuk; Zhong, Jiateng; Yang, Han; Chen, Huiling; Yang, Yafeng; Lam, Su Shiung; Yan, Lijun; Styrishave, Bjarne; Ciesielski, Tomasz Maciej; Peng, Wan Xi; Sonne, Christian.
In: Environmental Research, Vol. 241, 117474, 2024.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Ancient forest plants possess cytotoxic properties causing liver cancer HepG2 cell apoptosis
AU - Yue, Xiaochen
AU - Ling Ma, Nyuk
AU - Zhong, Jiateng
AU - Yang, Han
AU - Chen, Huiling
AU - Yang, Yafeng
AU - Lam, Su Shiung
AU - Yan, Lijun
AU - Styrishave, Bjarne
AU - Ciesielski, Tomasz Maciej
AU - Peng, Wan Xi
AU - Sonne, Christian
N1 - Publisher Copyright: © 2023 The Authors
PY - 2024
Y1 - 2024
N2 - Here, we collected 154 plant species in China ancient forests looking for novel efficient bioactive compounds for cancer treatments. We found 600 bioactive phyto-chemicals that induce apoptosis of liver cancer cell in vitro. First, we screen the plant extract's in vitro cytotoxicity inhibition of cancer cell growth using in vitro HepG2 cell lines and MTT cytotoxicity. The results from these initial MTT in vitro cytotoxicity tests show that the most efficient plants towards hepatoma cytoxicity is Cephalotaxus sinensis, mint bush (Elsholtzia stauntonii) and winged spindle tree (Euonymus alatus). We then used in cell-counting kit-8 (CCK-8) to further understand in vivo tumor growth using nude mice and GC-MS and LC-QTOF-MS to analyze the composition of compounds in the extracts. Extracted chemically active molecules analyzed by network pharmacology showed inhibition on the growth of liver cancer cells by acting on multiple gene targets, which is different from the currently used traditional drugs acting on only one target of liver cancer cells. Extracts from Cephalotaxus sinensis, mint bush (Elsholtzia stauntonii) and winged spindle tree (Euonymus alatus) induce apoptosis in hepatoma cancer cell line HepG2 with a killing rate of more than 83% and a tumor size decrease by 62–67% and a killing rate of only 6% of normal hepatocyte LO2. This study highlight efficient candidate species for cancer treatment providing a basis for future development of novel plant-based drugs to help meeting several of the UN SDGs and planetary health.
AB - Here, we collected 154 plant species in China ancient forests looking for novel efficient bioactive compounds for cancer treatments. We found 600 bioactive phyto-chemicals that induce apoptosis of liver cancer cell in vitro. First, we screen the plant extract's in vitro cytotoxicity inhibition of cancer cell growth using in vitro HepG2 cell lines and MTT cytotoxicity. The results from these initial MTT in vitro cytotoxicity tests show that the most efficient plants towards hepatoma cytoxicity is Cephalotaxus sinensis, mint bush (Elsholtzia stauntonii) and winged spindle tree (Euonymus alatus). We then used in cell-counting kit-8 (CCK-8) to further understand in vivo tumor growth using nude mice and GC-MS and LC-QTOF-MS to analyze the composition of compounds in the extracts. Extracted chemically active molecules analyzed by network pharmacology showed inhibition on the growth of liver cancer cells by acting on multiple gene targets, which is different from the currently used traditional drugs acting on only one target of liver cancer cells. Extracts from Cephalotaxus sinensis, mint bush (Elsholtzia stauntonii) and winged spindle tree (Euonymus alatus) induce apoptosis in hepatoma cancer cell line HepG2 with a killing rate of more than 83% and a tumor size decrease by 62–67% and a killing rate of only 6% of normal hepatocyte LO2. This study highlight efficient candidate species for cancer treatment providing a basis for future development of novel plant-based drugs to help meeting several of the UN SDGs and planetary health.
KW - Forest
KW - Genes
KW - Planetary health
KW - Treatment
KW - UN SDGs
U2 - 10.1016/j.envres.2023.117474
DO - 10.1016/j.envres.2023.117474
M3 - Journal article
C2 - 37879390
AN - SCOPUS:85179813463
VL - 241
JO - Environmental Research
JF - Environmental Research
SN - 0013-9351
M1 - 117474
ER -
ID: 378755388