Analysis of selenium nanoparticles in human plasma by capillary electrophoresis hyphenated to inductively coupled plasma mass spectrometry
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Analysis of selenium nanoparticles in human plasma by capillary electrophoresis hyphenated to inductively coupled plasma mass spectrometry. / Gronbaek-Thorsen, Freja; Hansen, Rikke Holck; Ostergaard, Jesper; Gammelgaard, Bente; Moller, Laura Hyrup.
In: Analytical and Bioanalytical Chemistry, Vol. 413, No. 8, 2021, p. 2247-2255.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Analysis of selenium nanoparticles in human plasma by capillary electrophoresis hyphenated to inductively coupled plasma mass spectrometry
AU - Gronbaek-Thorsen, Freja
AU - Hansen, Rikke Holck
AU - Ostergaard, Jesper
AU - Gammelgaard, Bente
AU - Moller, Laura Hyrup
PY - 2021
Y1 - 2021
N2 - Nanoparticles (NPs) are increasingly applied in research and development of new therapies. Characterization of NP systems most often include size, shape, size distribution, and charge but information on the chemical stability of NPs and investigation of the presence of dissolved species is most often missing in efficacy studies due to lack of appropriate methods. In this study, a method based on capillary electrophoresis coupled to inductively coupled plasma mass spectrometry (CE-ICP-MS) was established for analysis of selenium (Se) NPs and dissolved Se species in aqueous media. Peak area and migration time precisions (RSD) of 1.4-3.0% and 1.0-2.6%, respectively, were obtained. CE-ICP-MS analysis of a commercially available SeNP suspension (Q-SeNP) revealed large amounts of selenite corresponding to 32% of the total Se content in the suspension, indicating considerable NP degradation upon storage. The CE-ICP-MS method was modified using a coated fused silica capillary in order to analyze SeNPs in human plasma. Peak area and migration time precisions (RSD) in the range of 3.3-10.7% and 0.8-2.8%, respectively, were achieved. Degradation of polyvinyl alcohol (PVA)-coated SeNPs to selenite in human plasma was demonstrated using the modified method. The amounts of SeNP and selenite were estimated based on a correction factor for the ICP-MS signals of PVA-SeNP and dissolved Se. To the best of our knowledge, this is the first study of SeNPs by CE-ICP-MS and highlights the potential of CE-ICP-MS for quantitative characterization of the behavior of SeNPs in biological media.
AB - Nanoparticles (NPs) are increasingly applied in research and development of new therapies. Characterization of NP systems most often include size, shape, size distribution, and charge but information on the chemical stability of NPs and investigation of the presence of dissolved species is most often missing in efficacy studies due to lack of appropriate methods. In this study, a method based on capillary electrophoresis coupled to inductively coupled plasma mass spectrometry (CE-ICP-MS) was established for analysis of selenium (Se) NPs and dissolved Se species in aqueous media. Peak area and migration time precisions (RSD) of 1.4-3.0% and 1.0-2.6%, respectively, were obtained. CE-ICP-MS analysis of a commercially available SeNP suspension (Q-SeNP) revealed large amounts of selenite corresponding to 32% of the total Se content in the suspension, indicating considerable NP degradation upon storage. The CE-ICP-MS method was modified using a coated fused silica capillary in order to analyze SeNPs in human plasma. Peak area and migration time precisions (RSD) in the range of 3.3-10.7% and 0.8-2.8%, respectively, were achieved. Degradation of polyvinyl alcohol (PVA)-coated SeNPs to selenite in human plasma was demonstrated using the modified method. The amounts of SeNP and selenite were estimated based on a correction factor for the ICP-MS signals of PVA-SeNP and dissolved Se. To the best of our knowledge, this is the first study of SeNPs by CE-ICP-MS and highlights the potential of CE-ICP-MS for quantitative characterization of the behavior of SeNPs in biological media.
KW - Capillary electrophoresis
KW - electrophoresis
KW - Inductively coupled mass spectrometry
KW - ICP-MS
KW - Selenium nanoparticles
KW - Human plasma
U2 - 10.1007/s00216-021-03196-9
DO - 10.1007/s00216-021-03196-9
M3 - Journal article
C2 - 33580829
VL - 413
SP - 2247
EP - 2255
JO - Analusis
JF - Analusis
SN - 0365-4877
IS - 8
ER -
ID: 261157070