TY - JOUR

T1 - Additive manufacturing of polymeric scaffolds for biomimetic cell membrane engineering

AU - Rovira, David Sabate

AU - Nielsen, Hanne Morck

AU - Taboryski, Rafael

AU - Bunea, Ada-Ioana

PY - 2021

Y1 - 2021

N2 - Additive manufacturing based on direct laser writing two-photon polymerization facilitates the fabrication of microstructures with full 3D design freedom. Here, this fabrication technique is exploited for engineering scaffolds accurately mimicking the shape and size of three types of human cells. The human cell models employed in the study were chosen to include a range of dimensions and different identifiable features to highlight the versatility of this fabrication approach, yet other cell shapes can easily be fabricated in similar manner. The design and fabrication parameters for the additive manufacturing process were optimized to obtain polymeric scaffolds with biomimetic shapes. After fabrication, the cell scaffolds were converted to polymer-cushioned model cell membranes through layer-by layer functionalization with a cationic polymer and a lipid bilayer. Scaffold functionalization was verified using con focal laser scanning microscopy. Polymer-cushioned model cell membranes supported on 3D scaffolds mimicking the shape of human cells are particularly suitable for membrane interaction studies where membrane curvature plays an important role. The aim of this study is to demonstrate the engineering of biomimetic cell membranes by high resolution additive manufacturing combined with surface functionalization. The interdisciplinary approach highlights the value of additive manufacturing as technological solution for challenges encountered in biomedical studies.(c) 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).

AB - Additive manufacturing based on direct laser writing two-photon polymerization facilitates the fabrication of microstructures with full 3D design freedom. Here, this fabrication technique is exploited for engineering scaffolds accurately mimicking the shape and size of three types of human cells. The human cell models employed in the study were chosen to include a range of dimensions and different identifiable features to highlight the versatility of this fabrication approach, yet other cell shapes can easily be fabricated in similar manner. The design and fabrication parameters for the additive manufacturing process were optimized to obtain polymeric scaffolds with biomimetic shapes. After fabrication, the cell scaffolds were converted to polymer-cushioned model cell membranes through layer-by layer functionalization with a cationic polymer and a lipid bilayer. Scaffold functionalization was verified using con focal laser scanning microscopy. Polymer-cushioned model cell membranes supported on 3D scaffolds mimicking the shape of human cells are particularly suitable for membrane interaction studies where membrane curvature plays an important role. The aim of this study is to demonstrate the engineering of biomimetic cell membranes by high resolution additive manufacturing combined with surface functionalization. The interdisciplinary approach highlights the value of additive manufacturing as technological solution for challenges encountered in biomedical studies.(c) 2021 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY license (http:// creativecommons.org/licenses/by/4.0/).

KW - Additive manufacturing

KW - Two-photon polymerization

KW - 3D design

KW - Model cell membrane

KW - Surface modification

U2 - 10.1016/j.matdes.2021.109486

DO - 10.1016/j.matdes.2021.109486

M3 - Journal article

VL - 201

JO - Materials and Design

JF - Materials and Design

SN - 0264-1275

M1 - 109486

ER -