A neutral polyacrylate copolymer coating for surface modification of thiol-ene microchannels for improved performance of protein separation by microchip electrophoresis

Research output: Contribution to journalJournal articleResearchpeer-review

  • Kiarach Mesbah
  • Thanh Duc Mai
  • Thomas Glasdam Jensen
  • Laura Sola
  • Marcella Chiari
  • Kutter, Jörg P.
  • Myriam Taverna

We have investigated the behavior of thiol-ene substrates that is a class of promising materials for lab-on-a-chip electrophoresis applications. Two polymeric materials were prepared by copolymerization of N,N-dimethylacrylamide (DMA), (3-(methacryloyl-oxy)propyl)trimethoxysilane (PMA) and 3-trimethylsilanyl-prop-2-yne methacrylate (MAPS) and specifically adapted to thiol-ene formulations in order to obtain a neutral and permanent coating for microchannels. The performance of two different thiol-ene substrates (with 20 % and 40 % excess of thiol groups, respectively) coated with either p-(DMA-PMA) or p-(DMA-PMA-MAPS) copolymer were evaluated in terms of surface hydrophilicity, suppression and stability of electro-osmotic flow and prevention of protein adsorption. Surface modification of thiol-ene containing a 20 % excess of thiols with the terpolymer p-(DMA-PMA-MAPS) was found to offer the most stable coating and most efficient charge shielding in the pH range from 3 to 9. The modified microchannels were successfully applied to electrokinetic separations of acidic and basic proteins. [Figure not available: see fulltext.]

Original languageEnglish
JournalMicrochimica Acta
Issue number7
Pages (from-to)2111-2121
Number of pages11
Publication statusPublished - 1 Jul 2016

    Research areas

  • 3-(Methacryloyl-oxy)propyl-trimethoxysilane, 3-Trimethylsilanyl-prop-2-yne methacrylate, Electro-osmotic flow, N,N-Dimethylacrylamide, Protein adsorption, Protein separation, Surface hydrophilicity, Thiol-ene polymers

ID: 173280058