A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method

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A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method. / Könnings, Stephanie; Copland, Melissa J; Davies, Nigel M; Rades, Thomas.

In: International Journal of Pharmaceutics, Vol. 241, No. 2, 25.07.2002, p. 385-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Könnings, S, Copland, MJ, Davies, NM & Rades, T 2002, 'A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method', International Journal of Pharmaceutics, vol. 241, no. 2, pp. 385-9.

APA

Könnings, S., Copland, M. J., Davies, N. M., & Rades, T. (2002). A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method. International Journal of Pharmaceutics, 241(2), 385-9.

Vancouver

Könnings S, Copland MJ, Davies NM, Rades T. A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method. International Journal of Pharmaceutics. 2002 Jul 25;241(2):385-9.

Author

Könnings, Stephanie ; Copland, Melissa J ; Davies, Nigel M ; Rades, Thomas. / A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method. In: International Journal of Pharmaceutics. 2002 ; Vol. 241, No. 2. pp. 385-9.

Bibtex

@article{5b56832bddbf40ce8ca4ab965549499f,
title = "A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method",
abstract = "This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.",
keywords = "Adjuvants, Immunologic, Drug Delivery Systems, Fluorescein-5-isothiocyanate, Fluorescent Dyes, Ovalbumin, Saponins",
author = "Stephanie K{\"o}nnings and Copland, {Melissa J} and Davies, {Nigel M} and Thomas Rades",
year = "2002",
month = jul,
day = "25",
language = "English",
volume = "241",
pages = "385--9",
journal = "International Journal of Pharmaceutics",
issn = "0378-5173",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - A method for the incorporation of ovalbumin into immune stimulating complexes prepared by the hydration method

AU - Könnings, Stephanie

AU - Copland, Melissa J

AU - Davies, Nigel M

AU - Rades, Thomas

PY - 2002/7/25

Y1 - 2002/7/25

N2 - This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.

AB - This study describes the development of a method for the incorporation of fluorescently labelled ovalbumin (FITC-OVA) into immune stimulating complexes (ISCOMs) prepared by the hydration method. Conjugation of palmitic acid was performed to fluorescently labelled OVA (pFITC-OVA) or to non-labelled OVA, with subsequent conjugation of FITC to the resulting palmitified OVA (FITC-pOVA). Both pFITC-OVA and FITC-pOVA, but not FITC-OVA, could be incorporated into ISCOMs and other non-liposomal colloidal structures. The degree of incorporation of pFITC-OVA or FITC-pOVA in non-liposomal colloidal particles reaches a maximum, if ISCOMs are the predominant colloids in the system.

KW - Adjuvants, Immunologic

KW - Drug Delivery Systems

KW - Fluorescein-5-isothiocyanate

KW - Fluorescent Dyes

KW - Ovalbumin

KW - Saponins

M3 - Journal article

C2 - 12100866

VL - 241

SP - 385

EP - 389

JO - International Journal of Pharmaceutics

JF - International Journal of Pharmaceutics

SN - 0378-5173

IS - 2

ER -

ID: 46408585