Parameters affecting electro membrane extraction of basic drugs
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Parameters affecting electro membrane extraction of basic drugs. / Middelthon-Bruer, Torunn M.; Gjelstad, Astrid; Rasmussen, Knut E.; Pedersen-Bjergaard, Stig.
In: Journal of Separation Science, Vol. 31, No. 4, 01.03.2008, p. 753-759.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Parameters affecting electro membrane extraction of basic drugs
AU - Middelthon-Bruer, Torunn M.
AU - Gjelstad, Astrid
AU - Rasmussen, Knut E.
AU - Pedersen-Bjergaard, Stig
PY - 2008/3/1
Y1 - 2008/3/1
N2 - Thirty-five different basic drugs were extracted by electro membrane extraction (EME), from acidified samples containing HCl as the BGE, through an organic solvent immobilized in the pores in the wall of a porous hollow fiber (supported liquid membrane, SLM), and into an acidified acceptor solution (HCl) in the lumen of the hollow fiber by the application of an electrical potential difference of 50 V. With 2-nitrophenyl pentyl ether (NPPE) as the SLM, and with 10 mM HCl as BGE in the sample and acceptor solution, singly charged basic drugs with log P >2 were extracted with recoveries in the range 30-81% within 5 min. For doubly charged basic drugs, extraction was effectively enhanced by decreasing the concentration of HCl in the sample from 10 to 0.1 mM, reducing the ionization of the analytes. For medium polar analytes (1 < log P < 2), an ion balance of 0.01 was combined with addition of tris-(2-ethylhexyl) phosphate (TEHP) to the SLM, and this provided recoveries in the range 36-70%. The ion balance was defined as the concentration ratio of BGE between the sample and the acceptor solution. For the most polar drugs (log P <1), EME was accomplished with an ion balance of 0.01 and with di-(2-ethylhexyl) phosphate (DEHP) added to the SLM, but in spite of this, recoveries were in the range of only 4-17%.
AB - Thirty-five different basic drugs were extracted by electro membrane extraction (EME), from acidified samples containing HCl as the BGE, through an organic solvent immobilized in the pores in the wall of a porous hollow fiber (supported liquid membrane, SLM), and into an acidified acceptor solution (HCl) in the lumen of the hollow fiber by the application of an electrical potential difference of 50 V. With 2-nitrophenyl pentyl ether (NPPE) as the SLM, and with 10 mM HCl as BGE in the sample and acceptor solution, singly charged basic drugs with log P >2 were extracted with recoveries in the range 30-81% within 5 min. For doubly charged basic drugs, extraction was effectively enhanced by decreasing the concentration of HCl in the sample from 10 to 0.1 mM, reducing the ionization of the analytes. For medium polar analytes (1 < log P < 2), an ion balance of 0.01 was combined with addition of tris-(2-ethylhexyl) phosphate (TEHP) to the SLM, and this provided recoveries in the range 36-70%. The ion balance was defined as the concentration ratio of BGE between the sample and the acceptor solution. For the most polar drugs (log P <1), EME was accomplished with an ion balance of 0.01 and with di-(2-ethylhexyl) phosphate (DEHP) added to the SLM, but in spite of this, recoveries were in the range of only 4-17%.
KW - Basic drugs
KW - Electro membrane extraction
KW - Sample preparation
KW - Supported liquid membranes
UR - http://www.scopus.com/inward/record.url?scp=41049101213&partnerID=8YFLogxK
U2 - 10.1002/jssc.200700502
DO - 10.1002/jssc.200700502
M3 - Journal article
C2 - 18240134
AN - SCOPUS:41049101213
VL - 31
SP - 753
EP - 759
JO - HRC & CC, Journal of High Resolution Chromatography and Chromatography Communications
JF - HRC & CC, Journal of High Resolution Chromatography and Chromatography Communications
SN - 1615-9306
IS - 4
ER -
ID: 231650385