Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax

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Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax. / Rwegoshora, F.; Msengwa, Z.; Mabiki, F.; Machumi, F.; Chacha, M.; Styrishave, B.; Cornett, C.

In: East African Journal of Science, Technology and Innovation, Vol. 4, No. 2, 2023.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Rwegoshora, F, Msengwa, Z, Mabiki, F, Machumi, F, Chacha, M, Styrishave, B & Cornett, C 2023, 'Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax', East African Journal of Science, Technology and Innovation, vol. 4, no. 2. https://doi.org/10.37425/eajsti.v4i3.669

APA

Rwegoshora, F., Msengwa, Z., Mabiki, F., Machumi, F., Chacha, M., Styrishave, B., & Cornett, C. (2023). Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax. East African Journal of Science, Technology and Innovation, 4(2). https://doi.org/10.37425/eajsti.v4i3.669

Vancouver

Rwegoshora F, Msengwa Z, Mabiki F, Machumi F, Chacha M, Styrishave B et al. Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax. East African Journal of Science, Technology and Innovation. 2023;4(2). https://doi.org/10.37425/eajsti.v4i3.669

Author

Rwegoshora, F. ; Msengwa, Z. ; Mabiki, F. ; Machumi, F. ; Chacha, M. ; Styrishave, B. ; Cornett, C. / Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax. In: East African Journal of Science, Technology and Innovation. 2023 ; Vol. 4, No. 2.

Bibtex

@article{096ff895161146f686df9abe6c9a102f,
title = "Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax",
abstract = "Extracts of Synadenium glaucescens Pax are reported to contain biologically active compounds against bacterial and viral infections. This study aimed at isolating pure compounds from its bioactive extracts as well as evaluating their antibacterial efficacy. A phytochemical investigation of the root and stem barks involved total maceration in methanol (MeOH). The root barks extract was then partitioned by Vacuum Liquid Chromatography (VLC) in a solvent gradient system to afford three fractions of Hexane (Hex), Ethyl acetate (EtOAc) and finally MeOH for the least, moderate, and most polar compounds respectively. The MeOH extract of stem barks was also partitioned in the same way by using two solvents: dichloromethane (DCM) and MeOH. All fractions were finally dried on a rotary evaporator at < 60 OC of the water bath. Isolation of pure compound from the EtOAc and DCM led to isolation of four compounds namely, hexacosane (G1), β-sitosterol (G2), octacosyl ferulate (G3) and hexacosanoic acid (G4). Their structures were analyzed and confirmed through NMR, GC-MS and in comparison, with literature. Antibacterial assay for G1, G3 and G4 against P. aeruginosa, E. coli, S. aureus, and E. faecalis was achieved by broth serial microdilution. Compounds, G3 demonstrated strong activity against S. aureus (MIC = 0.125 mg/mL) and weak activity against the rest strains (MIC = 2 mg/ mL). Also, the test results indicated G1 had weak activity against all tested strains (MIC = 2 mg/mL or above). While G4 demonstrated a moderate activity (1.0 mg/mL) against E. coli, S. aureus, E. faecalis and weak against P. aeruginosa (MIC > 2 mg/mL). These findings support traditional use and promise for antibacterial drug agents from S. glaucescens Pax.",
keywords = "antimicrobial, drug, Euphorbiaceae, ferulic acid, NMR, octacosylferulate, phytochemistry",
author = "F. Rwegoshora and Z. Msengwa and F. Mabiki and F. Machumi and M. Chacha and B. Styrishave and C. Cornett",
note = "Publisher Copyright: {\textcopyright} 2023, East African Science and Technology Commission - EASTECO KG. All rights reserved.",
year = "2023",
doi = "10.37425/eajsti.v4i3.669",
language = "English",
volume = "4",
journal = "East African Journal of Science, Technology and Innovation",
issn = "2707-0425",
publisher = "East African Science and Technology Commission - EASTECO KG",
number = "2",

}

RIS

TY - JOUR

T1 - Isolation and elucidation of antibacterial compounds from roots and stems of Synadenium glaucescens Pax

AU - Rwegoshora, F.

AU - Msengwa, Z.

AU - Mabiki, F.

AU - Machumi, F.

AU - Chacha, M.

AU - Styrishave, B.

AU - Cornett, C.

N1 - Publisher Copyright: © 2023, East African Science and Technology Commission - EASTECO KG. All rights reserved.

PY - 2023

Y1 - 2023

N2 - Extracts of Synadenium glaucescens Pax are reported to contain biologically active compounds against bacterial and viral infections. This study aimed at isolating pure compounds from its bioactive extracts as well as evaluating their antibacterial efficacy. A phytochemical investigation of the root and stem barks involved total maceration in methanol (MeOH). The root barks extract was then partitioned by Vacuum Liquid Chromatography (VLC) in a solvent gradient system to afford three fractions of Hexane (Hex), Ethyl acetate (EtOAc) and finally MeOH for the least, moderate, and most polar compounds respectively. The MeOH extract of stem barks was also partitioned in the same way by using two solvents: dichloromethane (DCM) and MeOH. All fractions were finally dried on a rotary evaporator at < 60 OC of the water bath. Isolation of pure compound from the EtOAc and DCM led to isolation of four compounds namely, hexacosane (G1), β-sitosterol (G2), octacosyl ferulate (G3) and hexacosanoic acid (G4). Their structures were analyzed and confirmed through NMR, GC-MS and in comparison, with literature. Antibacterial assay for G1, G3 and G4 against P. aeruginosa, E. coli, S. aureus, and E. faecalis was achieved by broth serial microdilution. Compounds, G3 demonstrated strong activity against S. aureus (MIC = 0.125 mg/mL) and weak activity against the rest strains (MIC = 2 mg/ mL). Also, the test results indicated G1 had weak activity against all tested strains (MIC = 2 mg/mL or above). While G4 demonstrated a moderate activity (1.0 mg/mL) against E. coli, S. aureus, E. faecalis and weak against P. aeruginosa (MIC > 2 mg/mL). These findings support traditional use and promise for antibacterial drug agents from S. glaucescens Pax.

AB - Extracts of Synadenium glaucescens Pax are reported to contain biologically active compounds against bacterial and viral infections. This study aimed at isolating pure compounds from its bioactive extracts as well as evaluating their antibacterial efficacy. A phytochemical investigation of the root and stem barks involved total maceration in methanol (MeOH). The root barks extract was then partitioned by Vacuum Liquid Chromatography (VLC) in a solvent gradient system to afford three fractions of Hexane (Hex), Ethyl acetate (EtOAc) and finally MeOH for the least, moderate, and most polar compounds respectively. The MeOH extract of stem barks was also partitioned in the same way by using two solvents: dichloromethane (DCM) and MeOH. All fractions were finally dried on a rotary evaporator at < 60 OC of the water bath. Isolation of pure compound from the EtOAc and DCM led to isolation of four compounds namely, hexacosane (G1), β-sitosterol (G2), octacosyl ferulate (G3) and hexacosanoic acid (G4). Their structures were analyzed and confirmed through NMR, GC-MS and in comparison, with literature. Antibacterial assay for G1, G3 and G4 against P. aeruginosa, E. coli, S. aureus, and E. faecalis was achieved by broth serial microdilution. Compounds, G3 demonstrated strong activity against S. aureus (MIC = 0.125 mg/mL) and weak activity against the rest strains (MIC = 2 mg/ mL). Also, the test results indicated G1 had weak activity against all tested strains (MIC = 2 mg/mL or above). While G4 demonstrated a moderate activity (1.0 mg/mL) against E. coli, S. aureus, E. faecalis and weak against P. aeruginosa (MIC > 2 mg/mL). These findings support traditional use and promise for antibacterial drug agents from S. glaucescens Pax.

KW - antimicrobial

KW - drug

KW - Euphorbiaceae

KW - ferulic acid

KW - NMR

KW - octacosylferulate

KW - phytochemistry

U2 - 10.37425/eajsti.v4i3.669

DO - 10.37425/eajsti.v4i3.669

M3 - Journal article

AN - SCOPUS:85181942874

VL - 4

JO - East African Journal of Science, Technology and Innovation

JF - East African Journal of Science, Technology and Innovation

SN - 2707-0425

IS - 2

ER -

ID: 391675790