Design and characterization of core-shell mPEG-PLGA composite microparticles for development of cell-scaffold constructs
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Design and characterization of core-shell mPEG-PLGA composite microparticles for development of cell-scaffold constructs. / Wen, Yanhong; Gallego, Monica Ramos; Nielsen, Lene Feldskov; Jorgensen, Lene; Møller, Eva Horn; Nielsen, Hanne Mørck.
In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 85, No. 1, 09.2013, p. 87-98.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Design and characterization of core-shell mPEG-PLGA composite microparticles for development of cell-scaffold constructs
AU - Wen, Yanhong
AU - Gallego, Monica Ramos
AU - Nielsen, Lene Feldskov
AU - Jorgensen, Lene
AU - Møller, Eva Horn
AU - Nielsen, Hanne Mørck
N1 - Copyright © 2013 Elsevier B.V. All rights reserved.
PY - 2013/9
Y1 - 2013/9
N2 - Appropriate scaffolds capable of providing suitable biological and structural guidance are of great importance to generate cell-scaffold constructs for cell-based tissue engineering. The aim of the present study was to develop composite microparticles with a structure to provide functionality as a combined drug delivery/scaffold system. Composite microparticles were produced by incorporating either alginate/dermatan sulfate (Alg/DS) or alginate/chitosan/dermatan sulfate (Alg/CS/DS) particles in mPEG-PLGA microparticles using coaxial ultrasonic atomization. The encapsulation and distribution of Alg/DS or Alg/CS/DS particles in the mPEG-PLGA microparticles were significantly dependent on the operating conditions, including the flow rate ratio (Qout/Qin) and the viscosity of the polymer solutions (Vout, Vin) between the outer and the inner feeding channels. The core-shell composite microparticles containing the Alg/DS particles or the Alg/CS/DS particles displayed 40% and 65% DS release in 10 days, respectively, as compared to the DS directly loaded microparticles showing 90% DS release during the same time interval. The release profiles of DS correlate with the cell proliferation of fibroblasts, i.e. more sustainable cell growth was induced by the DS released from the core-shell composite microparticles comprising Alg/CS/DS particles. After seeding fibroblasts onto the composite microparticles, excellent cell adhesion was observed, and a successful assembly of the cell-scaffold constructs was induced within 7 days. Therefore, the present study demonstrates a novel strategy for fabrication of core-shell composite microparticles comprising additional particulate drug carriers in the core, which provides controlled delivery of DS and favorable cell biocompatibility; an approach to potentially achieve cell-based tissue regeneration.
AB - Appropriate scaffolds capable of providing suitable biological and structural guidance are of great importance to generate cell-scaffold constructs for cell-based tissue engineering. The aim of the present study was to develop composite microparticles with a structure to provide functionality as a combined drug delivery/scaffold system. Composite microparticles were produced by incorporating either alginate/dermatan sulfate (Alg/DS) or alginate/chitosan/dermatan sulfate (Alg/CS/DS) particles in mPEG-PLGA microparticles using coaxial ultrasonic atomization. The encapsulation and distribution of Alg/DS or Alg/CS/DS particles in the mPEG-PLGA microparticles were significantly dependent on the operating conditions, including the flow rate ratio (Qout/Qin) and the viscosity of the polymer solutions (Vout, Vin) between the outer and the inner feeding channels. The core-shell composite microparticles containing the Alg/DS particles or the Alg/CS/DS particles displayed 40% and 65% DS release in 10 days, respectively, as compared to the DS directly loaded microparticles showing 90% DS release during the same time interval. The release profiles of DS correlate with the cell proliferation of fibroblasts, i.e. more sustainable cell growth was induced by the DS released from the core-shell composite microparticles comprising Alg/CS/DS particles. After seeding fibroblasts onto the composite microparticles, excellent cell adhesion was observed, and a successful assembly of the cell-scaffold constructs was induced within 7 days. Therefore, the present study demonstrates a novel strategy for fabrication of core-shell composite microparticles comprising additional particulate drug carriers in the core, which provides controlled delivery of DS and favorable cell biocompatibility; an approach to potentially achieve cell-based tissue regeneration.
KW - Alginates
KW - Anticoagulants
KW - Biocompatible Materials
KW - Cell Adhesion
KW - Cell Proliferation
KW - Cells, Cultured
KW - Chitosan
KW - Dermatan Sulfate
KW - Drug Compounding
KW - Drug Delivery Systems
KW - Fibroblasts
KW - Glucuronic Acid
KW - Guided Tissue Regeneration
KW - Hexuronic Acids
KW - Humans
KW - Materials Testing
KW - Microspheres
KW - Particle Size
KW - Polyesters
KW - Polyethylene Glycols
KW - Solubility
KW - Surface Properties
KW - Tissue Engineering
KW - Tissue Scaffolds
KW - Viscosity
U2 - 10.1016/j.ejpb.2013.03.027
DO - 10.1016/j.ejpb.2013.03.027
M3 - Journal article
C2 - 23958320
VL - 85
SP - 87
EP - 98
JO - European Journal of Pharmaceutics and Biopharmaceutics
JF - European Journal of Pharmaceutics and Biopharmaceutics
SN - 0939-6411
IS - 1
ER -
ID: 104572438