Rapid photochemical surface patterning of proteins in thiol-ene based microfluidic devices
Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
The ability to immobilize biomolecules at specific locations on the surface of solid supports is central to many biochip applications. This paper reports the rapid one-step photochemical surface patterning of biomolecules in thiol-ene microfluidic chips. Adjusting the stoichiometric ratio of "thiol" and "ene" monomers present in the microfluidic chip bulk material provides a simple and efficient way of tuning the chip's surface chemistry. Here, thiol-ene chips displaying an excess of functional thiol groups at their surfaces are functionalized with biotin and streptavidin in a controlled fashion using photolithography. We also present quantitative data on the number of functional groups available for surface modification on thiol-ene substrates and their stability.
Original language | English |
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Title of host publication | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 |
Number of pages | 3 |
Publisher | Chemical and Biological Microsystems Society |
Publication date | 1 Jan 2012 |
Pages | 1258-1260 |
ISBN (Print) | 9780979806452 |
Publication status | Published - 1 Jan 2012 |
- Biochips, Biomolecule immobilization, Surface functionalization, Thiol-ene polymers
Research areas
ID: 117304652