Human Lipoproteins at Model Cell Membranes: Effect of Lipoprotein Class on Lipid Exchange
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Human Lipoproteins at Model Cell Membranes : Effect of Lipoprotein Class on Lipid Exchange. / Browning, Kathryn; Lind, T K; Maric, S; Malekkhaiat-Häffner, S; Fredrikson, G N; Bengtsson, E; Malmsten, M; Cárdenas, M.
In: Scientific Reports, Vol. 7, No. 1, 7478, 07.08.2017.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Human Lipoproteins at Model Cell Membranes
T2 - Effect of Lipoprotein Class on Lipid Exchange
AU - Browning, Kathryn
AU - Lind, T K
AU - Maric, S
AU - Malekkhaiat-Häffner, S
AU - Fredrikson, G N
AU - Bengtsson, E
AU - Malmsten, M
AU - Cárdenas, M
PY - 2017/8/7
Y1 - 2017/8/7
N2 - High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the 'good' cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the 'bad' cholesterol, depositing lipid material into the vascular wall.
AB - High and low density lipoproteins (HDL and LDL) are thought to play vital roles in the onset and development of atherosclerosis; the biggest killer in the western world. Key issues of initial lipoprotein (LP) interactions at cellular membranes need to be addressed including LP deposition and lipid exchange. Here we present a protocol for monitoring the in situ kinetics of lipoprotein deposition and lipid exchange/removal at model cellular membranes using the non-invasive, surface sensitive methods of neutron reflection and quartz crystal microbalance with dissipation. For neutron reflection, lipid exchange and lipid removal can be distinguished thanks to the combined use of hydrogenated and tail-deuterated lipids. Both HDL and LDL remove lipids from the bilayer and deposit hydrogenated material into the lipid bilayer, however, the extent of removal and exchange depends on LP type. These results support the notion of HDL acting as the 'good' cholesterol, removing lipid material from lipid-loaded cells, whereas LDL acts as the 'bad' cholesterol, depositing lipid material into the vascular wall.
KW - Journal Article
U2 - 10.1038/s41598-017-07505-0
DO - 10.1038/s41598-017-07505-0
M3 - Journal article
C2 - 28785025
VL - 7
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 7478
ER -
ID: 185029872