Comparison of biodistribution of cerium oxide nanoparticles after repeated oral administration by gavage or snack in Sprague Dawley rats
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Comparison of biodistribution of cerium oxide nanoparticles after repeated oral administration by gavage or snack in Sprague Dawley rats. / Berthing, Trine; Holmfred, Else; Vidmar, Janja; Hadrup, Niels; Mortensen, Alicja; Szarek, Józef; Loeschner, Katrin; Vogel, Ulla.
In: Environmental Toxicology and Pharmacology, Vol. 95, 103939, 2022.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Comparison of biodistribution of cerium oxide nanoparticles after repeated oral administration by gavage or snack in Sprague Dawley rats
AU - Berthing, Trine
AU - Holmfred, Else
AU - Vidmar, Janja
AU - Hadrup, Niels
AU - Mortensen, Alicja
AU - Szarek, Józef
AU - Loeschner, Katrin
AU - Vogel, Ulla
N1 - Funding Information: This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 760813 (PATROLS) and FFIKA, Focused Research Effort on Chemicals in the Working Environment , from the Danish Government. Funding Information: This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 760813 (PATROLS) and FFIKA, Focused Research Effort on Chemicals in the Working Environment, from the Danish Government. Publisher Copyright: © 2022 The Authors
PY - 2022
Y1 - 2022
N2 - The rate of translocation of ingested nanoparticles (NPs) and how the uptake is affected by a food matrix are key aspects of health risk assessment. In this study, female Sprague Dawley rats (N = 4/group) received 0, 1.4, or 13 mg of cerium oxide (CeO2 NM-212) NPs/rat/day by gavage or in a chocolate spread snack 5 days/week for 1 or 2 weeks followed by 2 weeks of recovery. A dose and time-dependent uptake in the liver and spleen of 0.1–0.3 and 0.004–0.005 parts per million (ng/mg) of the total administered dose was found, respectively. There was no statistically significant difference in cerium concentration in the liver or spleen after gavage compared to snack dosing. Microscopy revealed indications of necrotic changes in the liver and decreased cellularity in white pulp in the spleen. The snack provided precise administration and a more human-relevant exposure of NPs and could improve animal welfare as alternative to gavage.
AB - The rate of translocation of ingested nanoparticles (NPs) and how the uptake is affected by a food matrix are key aspects of health risk assessment. In this study, female Sprague Dawley rats (N = 4/group) received 0, 1.4, or 13 mg of cerium oxide (CeO2 NM-212) NPs/rat/day by gavage or in a chocolate spread snack 5 days/week for 1 or 2 weeks followed by 2 weeks of recovery. A dose and time-dependent uptake in the liver and spleen of 0.1–0.3 and 0.004–0.005 parts per million (ng/mg) of the total administered dose was found, respectively. There was no statistically significant difference in cerium concentration in the liver or spleen after gavage compared to snack dosing. Microscopy revealed indications of necrotic changes in the liver and decreased cellularity in white pulp in the spleen. The snack provided precise administration and a more human-relevant exposure of NPs and could improve animal welfare as alternative to gavage.
KW - ICP-MS
KW - In vivo
KW - Nanoceria
KW - Nanomaterial
KW - Oral exposure
KW - Toxicokinetics
KW - Translocation
U2 - 10.1016/j.etap.2022.103939
DO - 10.1016/j.etap.2022.103939
M3 - Journal article
C2 - 35908641
AN - SCOPUS:85135942740
VL - 95
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
SN - 1382-6689
M1 - 103939
ER -
ID: 365844498