A Skin Cancer Prophylaxis Study in Hairless Mice Using Methylene Blue, Riboflavin, and Methyl Aminolevulinate as Photosensitizing Agents in Photodynamic Therapy

Research output: Contribution to journalJournal articlepeer-review

Documents

The high incidence of sunlight-induced human skin cancers reveals a need for more effective photosensitizing agents. In this study, we compared the efficacy of prophylactic photodynamic therapy (PDT) when methylene blue (MB), riboflavin (RF), or methyl aminolevulinate (MAL) were used as photosensitizers. All mice in four groups of female C3.Cg/TifBomTac hairless immunocompetent mice (N = 100) were irradiated with three standard erythema doses of solar-simulated ultraviolet radiation (UVR) thrice weekly. Three groups received 2 x 2 prophylactic PDT treatments (days 45 + 52 and 90 + 97). The PDT treatments consisted of topical administration of 16% MAL, 20% MB, or 20% RF, and subsequent illumination that matched the photosensitizers' absorption spectra. Control mice received no PDT. We recorded when the first, second, and third skin tumors developed. The pattern of tumor development after MB-PDT or RF-PDT was similar to that observed in irradiated control mice (p > 0.05). However, the median times until the first, second, and third skin tumors developed in mice given MAL-PDT were significantly delayed, compared with control mice (256, 265, and 272 vs. 215, 222, and 230 days, respectively; p < 0.001). Only MAL-PDT was an effective prophylactic treatment against UVR-induced skin tumors in hairless mice.

Original languageEnglish
Article number433
JournalPharmaceuticals
Volume14
Issue number5
Number of pages11
ISSN1424-8247
DOIs
Publication statusPublished - May 2021

    Research areas

  • PDT, methyl aminolevulinate, methylene blue, riboflavin, photosensitizing agents, ultraviolet radiation, skin tumors, prophylactic treatment, hairless mice, BASAL-CELL CARCINOMA, ACTINIC KERATOSES, DELIVERY, PHOTOCARCINOGENESIS, PHOTOACTIVATION, MULTICENTER, EXPOSURE, SURGERY

Number of downloads are based on statistics from Google Scholar and www.ku.dk


No data available

ID: 272404503