Quantification of Proteins and Peptides in Drug Formulations (PhD Project) – University of Copenhagen

Quantification of Proteins and Peptides in Drug Formulations (PhD Project)

Proteins and peptides are gaining momentum in the treatment of a number of serious diseases and disorders, either functioning as active drug substances or as part of delivery systems. As these molecules are difficult to quantify, improved quantitative analytical methods are needed for the study of protein and peptide metabolism as well as in characterization of drug delivery systems.

By introduction of natural element labels to biomolecules, quantitative measurements can be performed by use of element/isotope specific ICP-MS (Inductively Coupled Plasma Mass Spectrometry) detection, exploiting the high and structure-independent sensitivity, multi-element capability and wide dynamic range of this technique. By hyphenation to HPLC (high performance liquid chromatography), stability, pharmacokinetics and metabolism can be monitored in complex biological samples.

The aim is to develop robust methods by use of labels that cause minimal disturbance of the natural properties of the biomolecule of interest, unequivocal labelling stoichiometry and low limits of detection. Thus, improved information of the fate of biomolecules in biological systems may be obtainable.