Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia

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Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia. / Nielsen, Mette M B; Lambertsen, Kate L; Clausen, Bettina H; Meyer, Morten; Bhandari, Dhaka R; Larsen, Søren T; Poulsen, Steen S; Spengler, Bernhard; Janfelt, Christian; Hansen, Harald S.

In: Scientific Reports, Vol. 6, 39571, 22.12.2016, p. 1-14.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Nielsen, MMB, Lambertsen, KL, Clausen, BH, Meyer, M, Bhandari, DR, Larsen, ST, Poulsen, SS, Spengler, B, Janfelt, C & Hansen, HS 2016, 'Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia', Scientific Reports, vol. 6, 39571, pp. 1-14. https://doi.org/10.1038/srep39571

APA

Nielsen, M. M. B., Lambertsen, K. L., Clausen, B. H., Meyer, M., Bhandari, D. R., Larsen, S. T., ... Hansen, H. S. (2016). Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia. Scientific Reports, 6, 1-14. [39571]. https://doi.org/10.1038/srep39571

Vancouver

Nielsen MMB, Lambertsen KL, Clausen BH, Meyer M, Bhandari DR, Larsen ST et al. Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia. Scientific Reports. 2016 Dec 22;6:1-14. 39571. https://doi.org/10.1038/srep39571

Author

Nielsen, Mette M B ; Lambertsen, Kate L ; Clausen, Bettina H ; Meyer, Morten ; Bhandari, Dhaka R ; Larsen, Søren T ; Poulsen, Steen S ; Spengler, Bernhard ; Janfelt, Christian ; Hansen, Harald S. / Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia. In: Scientific Reports. 2016 ; Vol. 6. pp. 1-14.

Bibtex

@article{371e785a5767401aa196be4d5dc0ba98,
title = "Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia",
abstract = "Focal cerebral ischaemia has an initial phase of inflammation and tissue injury followed by a later phase of resolution and repair. Mass spectrometry imaging (desorption electrospray ionization and matrix assisted laser desorption ionization) was applied on brain sections from mice 2 h, 24 h, 5d, 7d, and 20d after permanent focal cerebral ischaemia. Within 24 h, N-acyl-phosphatidylethanolamines, lysophosphatidylcholine, and ceramide accumulated, while sphingomyelin disappeared. At the later resolution stages, bis(monoacylglycero)phosphate (BMP(22:6/22:6)), 2-arachidonoyl-glycerol, ceramide-phosphate, sphingosine-1-phosphate, lysophosphatidylserine, and cholesteryl ester appeared. At day 5 to 7, dihydroxy derivates of docosahexaenoic and docosapentaenoic acid, some of which may be pro-resolving mediators, e.g. resolvins, were found in the injured area, and BMP(22:6/22:6) co-localized with the macrophage biomarker CD11b, and probably with cholesteryl ester. Mass spectrometry imaging can visualize spatiotemporal changes in the lipidome during the progression and resolution of focal cerebral inflammation and suggests that BMP(22:6/22:6) and N-acyl-phosphatidylethanolamines can be used as biomarkers for phagocytizing macrophages/microglia cells and dead neurones, respectively.",
author = "Nielsen, {Mette M B} and Lambertsen, {Kate L} and Clausen, {Bettina H} and Morten Meyer and Bhandari, {Dhaka R} and Larsen, {S{\o}ren T} and Poulsen, {Steen S} and Bernhard Spengler and Christian Janfelt and Hansen, {Harald S}",
year = "2016",
month = "12",
day = "22",
doi = "10.1038/srep39571",
language = "English",
volume = "6",
pages = "1--14",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia

AU - Nielsen, Mette M B

AU - Lambertsen, Kate L

AU - Clausen, Bettina H

AU - Meyer, Morten

AU - Bhandari, Dhaka R

AU - Larsen, Søren T

AU - Poulsen, Steen S

AU - Spengler, Bernhard

AU - Janfelt, Christian

AU - Hansen, Harald S

PY - 2016/12/22

Y1 - 2016/12/22

N2 - Focal cerebral ischaemia has an initial phase of inflammation and tissue injury followed by a later phase of resolution and repair. Mass spectrometry imaging (desorption electrospray ionization and matrix assisted laser desorption ionization) was applied on brain sections from mice 2 h, 24 h, 5d, 7d, and 20d after permanent focal cerebral ischaemia. Within 24 h, N-acyl-phosphatidylethanolamines, lysophosphatidylcholine, and ceramide accumulated, while sphingomyelin disappeared. At the later resolution stages, bis(monoacylglycero)phosphate (BMP(22:6/22:6)), 2-arachidonoyl-glycerol, ceramide-phosphate, sphingosine-1-phosphate, lysophosphatidylserine, and cholesteryl ester appeared. At day 5 to 7, dihydroxy derivates of docosahexaenoic and docosapentaenoic acid, some of which may be pro-resolving mediators, e.g. resolvins, were found in the injured area, and BMP(22:6/22:6) co-localized with the macrophage biomarker CD11b, and probably with cholesteryl ester. Mass spectrometry imaging can visualize spatiotemporal changes in the lipidome during the progression and resolution of focal cerebral inflammation and suggests that BMP(22:6/22:6) and N-acyl-phosphatidylethanolamines can be used as biomarkers for phagocytizing macrophages/microglia cells and dead neurones, respectively.

AB - Focal cerebral ischaemia has an initial phase of inflammation and tissue injury followed by a later phase of resolution and repair. Mass spectrometry imaging (desorption electrospray ionization and matrix assisted laser desorption ionization) was applied on brain sections from mice 2 h, 24 h, 5d, 7d, and 20d after permanent focal cerebral ischaemia. Within 24 h, N-acyl-phosphatidylethanolamines, lysophosphatidylcholine, and ceramide accumulated, while sphingomyelin disappeared. At the later resolution stages, bis(monoacylglycero)phosphate (BMP(22:6/22:6)), 2-arachidonoyl-glycerol, ceramide-phosphate, sphingosine-1-phosphate, lysophosphatidylserine, and cholesteryl ester appeared. At day 5 to 7, dihydroxy derivates of docosahexaenoic and docosapentaenoic acid, some of which may be pro-resolving mediators, e.g. resolvins, were found in the injured area, and BMP(22:6/22:6) co-localized with the macrophage biomarker CD11b, and probably with cholesteryl ester. Mass spectrometry imaging can visualize spatiotemporal changes in the lipidome during the progression and resolution of focal cerebral inflammation and suggests that BMP(22:6/22:6) and N-acyl-phosphatidylethanolamines can be used as biomarkers for phagocytizing macrophages/microglia cells and dead neurones, respectively.

U2 - 10.1038/srep39571

DO - 10.1038/srep39571

M3 - Journal article

C2 - 28004822

VL - 6

SP - 1

EP - 14

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 39571

ER -

ID: 171548750