Imaging of plant materials using indirect desorption electrospray ionization mass spectrometry

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Indirect desorption electrospray ionization mass spectrometry (DESI-MS) imaging is a method for imaging distributions of metabolites in plant materials, in particular leaves and petals. The challenge in direct imaging of such plant materials with DESI-MS is particularly the protective layer of cuticular wax present in leaves and petals. The cuticle protects the plant from drying out, but also makes it difficult for the DESI sprayer to reach the analytes of interest inside the plant material. A solution to this problem is to imprint the plant material onto a surface, thus releasing the analytes of interest from parts of their matrix while preserving the spatial information in the two dimensions. The imprint can then easily be imaged by DESI-MS. The method delivers simple and robust mass spectrometry imaging of plant material with very high success ratios.

Original languageEnglish
Title of host publicationMass Spectrometry Imaging of Small Molecules
EditorsLin He
Number of pages7
PublisherHumana Press
Publication date2015
ISBN (Print)978-1-4939-1356-5
ISBN (Electronic)978-1-4939-1357-2
Publication statusPublished - 2015
SeriesMethods in Molecular Biology

ID: 162123018