Utilizing nanoparticles for improving anti-biofilm effects of azithromycin: A head-to-head comparison of modified hyaluronic acid nanogels and coated poly (lactic-co-glycolic acid) nanoparticles

Research output: Contribution to journalJournal articlepeer-review

Standard

Utilizing nanoparticles for improving anti-biofilm effects of azithromycin : A head-to-head comparison of modified hyaluronic acid nanogels and coated poly (lactic-co-glycolic acid) nanoparticles. / Kłodzińska, Sylvia N.; Wan, Feng; Jumaa, Haidar; Sternberg, Claus; Rades, Thomas; Nielsen, Hanne M.

In: Journal of Colloid and Interface Science, Vol. 555, 01.11.2019, p. 595-606.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Kłodzińska, SN, Wan, F, Jumaa, H, Sternberg, C, Rades, T & Nielsen, HM 2019, 'Utilizing nanoparticles for improving anti-biofilm effects of azithromycin: A head-to-head comparison of modified hyaluronic acid nanogels and coated poly (lactic-co-glycolic acid) nanoparticles', Journal of Colloid and Interface Science, vol. 555, pp. 595-606. https://doi.org/10.1016/j.jcis.2019.08.006

APA

Kłodzińska, S. N., Wan, F., Jumaa, H., Sternberg, C., Rades, T., & Nielsen, H. M. (2019). Utilizing nanoparticles for improving anti-biofilm effects of azithromycin: A head-to-head comparison of modified hyaluronic acid nanogels and coated poly (lactic-co-glycolic acid) nanoparticles. Journal of Colloid and Interface Science, 555, 595-606. https://doi.org/10.1016/j.jcis.2019.08.006

Vancouver

Kłodzińska SN, Wan F, Jumaa H, Sternberg C, Rades T, Nielsen HM. Utilizing nanoparticles for improving anti-biofilm effects of azithromycin: A head-to-head comparison of modified hyaluronic acid nanogels and coated poly (lactic-co-glycolic acid) nanoparticles. Journal of Colloid and Interface Science. 2019 Nov 1;555:595-606. https://doi.org/10.1016/j.jcis.2019.08.006

Author

Kłodzińska, Sylvia N. ; Wan, Feng ; Jumaa, Haidar ; Sternberg, Claus ; Rades, Thomas ; Nielsen, Hanne M. / Utilizing nanoparticles for improving anti-biofilm effects of azithromycin : A head-to-head comparison of modified hyaluronic acid nanogels and coated poly (lactic-co-glycolic acid) nanoparticles. In: Journal of Colloid and Interface Science. 2019 ; Vol. 555. pp. 595-606.

Bibtex

@article{7412fdbfa0f7444f919af9efc21fd6aa,
title = "Utilizing nanoparticles for improving anti-biofilm effects of azithromycin: A head-to-head comparison of modified hyaluronic acid nanogels and coated poly (lactic-co-glycolic acid) nanoparticles",
abstract = "Hypothesis: The widespread resistance of bacteria to traditional antibiotic treatments has expedited the search for novel therapies against these pathogens. The hypothesis of this work is that two distinctively different polymeric delivery systems, specifically D-α-tocopherol polyethylene glycol 1000 succinate (TPGS)-poly(lactic-co-glycolic acid) (PLGA) nanoparticles and octenyl succinic anhydride-modified low molecular weight hyaluronic acid (OSA-HA) nanogels may be used to substantially improve the properties of azithromycin, allowing its use for effective treatment of Pseudomonas aeruginosa biofilm infections. Experiments: Azithromycin was encapsulated in both delivery systems and the physicochemical properties of the loaded delivery systems, including size, surface charge and drug loading were evaluated. Additionally, particle interaction with a mucin layer, penetration into a bacterial biofilm, prevention of biofilm formation and eradication of pre-formed biofilms, the influence on production of virulence factors and bacterial motility as well as cytotoxicity towards hepatocytes and lung epithelial cells were compared head-to-head. Findings: The TPGS-PLGA nanoparticles noticeably improved the antimicrobial activity and the biofilm prevention activity of azithromycin whereas the OSA-HA nanogels showed reduced mucin interactions together with improved reduction of pre-formed biofilms and maintained the low eukaryotic cell cytotoxicity of azithromycin.",
keywords = "Antibiotic, Biofilm, Cystic fibrosis, Formulation, Macrolide, Nanogel",
author = "K{\l}odzi{\'n}ska, {Sylvia N.} and Feng Wan and Haidar Jumaa and Claus Sternberg and Thomas Rades and Nielsen, {Hanne M.}",
year = "2019",
month = nov,
day = "1",
doi = "10.1016/j.jcis.2019.08.006",
language = "English",
volume = "555",
pages = "595--606",
journal = "Journal of Colloid and Interface Science",
issn = "0021-9797",
publisher = "Academic Press",

}

RIS

TY - JOUR

T1 - Utilizing nanoparticles for improving anti-biofilm effects of azithromycin

T2 - A head-to-head comparison of modified hyaluronic acid nanogels and coated poly (lactic-co-glycolic acid) nanoparticles

AU - Kłodzińska, Sylvia N.

AU - Wan, Feng

AU - Jumaa, Haidar

AU - Sternberg, Claus

AU - Rades, Thomas

AU - Nielsen, Hanne M.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Hypothesis: The widespread resistance of bacteria to traditional antibiotic treatments has expedited the search for novel therapies against these pathogens. The hypothesis of this work is that two distinctively different polymeric delivery systems, specifically D-α-tocopherol polyethylene glycol 1000 succinate (TPGS)-poly(lactic-co-glycolic acid) (PLGA) nanoparticles and octenyl succinic anhydride-modified low molecular weight hyaluronic acid (OSA-HA) nanogels may be used to substantially improve the properties of azithromycin, allowing its use for effective treatment of Pseudomonas aeruginosa biofilm infections. Experiments: Azithromycin was encapsulated in both delivery systems and the physicochemical properties of the loaded delivery systems, including size, surface charge and drug loading were evaluated. Additionally, particle interaction with a mucin layer, penetration into a bacterial biofilm, prevention of biofilm formation and eradication of pre-formed biofilms, the influence on production of virulence factors and bacterial motility as well as cytotoxicity towards hepatocytes and lung epithelial cells were compared head-to-head. Findings: The TPGS-PLGA nanoparticles noticeably improved the antimicrobial activity and the biofilm prevention activity of azithromycin whereas the OSA-HA nanogels showed reduced mucin interactions together with improved reduction of pre-formed biofilms and maintained the low eukaryotic cell cytotoxicity of azithromycin.

AB - Hypothesis: The widespread resistance of bacteria to traditional antibiotic treatments has expedited the search for novel therapies against these pathogens. The hypothesis of this work is that two distinctively different polymeric delivery systems, specifically D-α-tocopherol polyethylene glycol 1000 succinate (TPGS)-poly(lactic-co-glycolic acid) (PLGA) nanoparticles and octenyl succinic anhydride-modified low molecular weight hyaluronic acid (OSA-HA) nanogels may be used to substantially improve the properties of azithromycin, allowing its use for effective treatment of Pseudomonas aeruginosa biofilm infections. Experiments: Azithromycin was encapsulated in both delivery systems and the physicochemical properties of the loaded delivery systems, including size, surface charge and drug loading were evaluated. Additionally, particle interaction with a mucin layer, penetration into a bacterial biofilm, prevention of biofilm formation and eradication of pre-formed biofilms, the influence on production of virulence factors and bacterial motility as well as cytotoxicity towards hepatocytes and lung epithelial cells were compared head-to-head. Findings: The TPGS-PLGA nanoparticles noticeably improved the antimicrobial activity and the biofilm prevention activity of azithromycin whereas the OSA-HA nanogels showed reduced mucin interactions together with improved reduction of pre-formed biofilms and maintained the low eukaryotic cell cytotoxicity of azithromycin.

KW - Antibiotic

KW - Biofilm

KW - Cystic fibrosis

KW - Formulation

KW - Macrolide

KW - Nanogel

UR - http://www.scopus.com/inward/record.url?scp=85070247588&partnerID=8YFLogxK

U2 - 10.1016/j.jcis.2019.08.006

DO - 10.1016/j.jcis.2019.08.006

M3 - Journal article

C2 - 31404843

AN - SCOPUS:85070247588

VL - 555

SP - 595

EP - 606

JO - Journal of Colloid and Interface Science

JF - Journal of Colloid and Interface Science

SN - 0021-9797

ER -

ID: 235004721