Transendothelial electrical resistance measurement across the blood–brain barrier: A critical review of methods
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Transendothelial electrical resistance measurement across the blood–brain barrier : A critical review of methods. / Vigh, Judit P.; Kincses, András; Ozgür, Burak; Walter, Fruzsina R.; Santa-Maria, Ana Raquel; Valkai, Sándor; Vastag, Mónika; Neuhaus, Winfried; Brodin, Birger; Dér, András; Deli, Mária A.
In: Micromachines, Vol. 12, No. 6, 685, 2021.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Transendothelial electrical resistance measurement across the blood–brain barrier
T2 - A critical review of methods
AU - Vigh, Judit P.
AU - Kincses, András
AU - Ozgür, Burak
AU - Walter, Fruzsina R.
AU - Santa-Maria, Ana Raquel
AU - Valkai, Sándor
AU - Vastag, Mónika
AU - Neuhaus, Winfried
AU - Brodin, Birger
AU - Dér, András
AU - Deli, Mária A.
N1 - Funding Information: This research was funded by the National Research, Development, and Innovation Office of Hungary grant numbers OTKA NNE 129617 (as part of the M-Era.NET2 nanoPD project to M.A.D.) and PD-128480 (to F.R.W.), by the Lundbeck Foundation, grant no. R155-2013-14113 (?Research Initiative on Brain Barriers and Drug Delivery?, to B.B.) and by the European Union?s Horizon 2020 research and innovation program Innovative Medicines Initiative (IMI), grant number 807015 (Innovative Medicines Initiative 2 Joint Undertaking, to B.B.). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021
Y1 - 2021
N2 - The blood–brain barrier (BBB) represents the tightest endothelial barrier within the cardio-vascular system characterized by very low ionic permeability. Our aim was to describe the setups, electrodes, and instruments to measure electrical resistance across brain microvessels and culture models of the BBB, as well as critically assess the influence of often neglected physical and technical parameters such as temperature, viscosity, current density generated by different electrode types, surface size, circumference, and porosity of the culture insert membrane. We demonstrate that these physical and technical parameters greatly influence the measurement of transendothelial electrical resistance/resistivity (TEER) across BBB culture models resulting in severalfold differences in TEER values of the same biological model, especially in the low-TEER range. We show that elevated culture medium viscosity significantly increases, while higher membrane porosity decreases TEER values. TEER data measured by chopstick electrodes can be threefold higher than values measured by chamber electrodes due to different electrode size and geometry, resulting in current distribution inhomogeneity. An additional shunt resistance at the circumference of culture inserts results in lower TEER values. A detailed description of setups and technical parameters is crucial for the correct interpretation and comparison of TEER values of BBB models.
AB - The blood–brain barrier (BBB) represents the tightest endothelial barrier within the cardio-vascular system characterized by very low ionic permeability. Our aim was to describe the setups, electrodes, and instruments to measure electrical resistance across brain microvessels and culture models of the BBB, as well as critically assess the influence of often neglected physical and technical parameters such as temperature, viscosity, current density generated by different electrode types, surface size, circumference, and porosity of the culture insert membrane. We demonstrate that these physical and technical parameters greatly influence the measurement of transendothelial electrical resistance/resistivity (TEER) across BBB culture models resulting in severalfold differences in TEER values of the same biological model, especially in the low-TEER range. We show that elevated culture medium viscosity significantly increases, while higher membrane porosity decreases TEER values. TEER data measured by chopstick electrodes can be threefold higher than values measured by chamber electrodes due to different electrode size and geometry, resulting in current distribution inhomogeneity. An additional shunt resistance at the circumference of culture inserts results in lower TEER values. A detailed description of setups and technical parameters is crucial for the correct interpretation and comparison of TEER values of BBB models.
KW - Blood–brain barrier
KW - Cell culture insert
KW - Electrodes
KW - Endothelial cell
KW - Epithelial cell
KW - Impedance
KW - Lab-on-a-chip
KW - Transendothelial electrical resistance
KW - Viscosity
U2 - 10.3390/mi12060685
DO - 10.3390/mi12060685
M3 - Review
C2 - 34208338
AN - SCOPUS:85108703107
VL - 12
JO - Micromachines
JF - Micromachines
SN - 2072-666X
IS - 6
M1 - 685
ER -
ID: 273537875