The physicochemical properties of preserved and preservative-free latanoprost eye drops and their impact on human conjunctival goblet cells

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The physicochemical properties of preserved and preservative-free latanoprost eye drops and their impact on human conjunctival goblet cells. / Müllertz, Olivia Amanda Oest; Nagstrup, Anne Hedengran; Mouhammad, Zaynab Ahmad; Freiberg, Josefine Clement; Nagymihály, Richárd; Jacobsen, Jette; Larsen, Susan Weng; Bair, Jeffrey A; Utheim, Tor Paaske; Dartt, Darlene A.; Heegaard, Steffen; Petrovski, Goran; Kolko, Miriam.

In: Acta Ophthalmologica, Vol. 100, No. S267, 2022.

Research output: Contribution to journalConference abstract in journalpeer-review

Harvard

Müllertz, OAO, Nagstrup, AH, Mouhammad, ZA, Freiberg, JC, Nagymihály, R, Jacobsen, J, Larsen, SW, Bair, JA, Utheim, TP, Dartt, DA, Heegaard, S, Petrovski, G & Kolko, M 2022, 'The physicochemical properties of preserved and preservative-free latanoprost eye drops and their impact on human conjunctival goblet cells', Acta Ophthalmologica, vol. 100, no. S267. https://doi.org/10.1111/j.1755-3768.2022.071

APA

Müllertz, O. A. O., Nagstrup, A. H., Mouhammad, Z. A., Freiberg, J. C., Nagymihály, R., Jacobsen, J., Larsen, S. W., Bair, J. A., Utheim, T. P., Dartt, D. A., Heegaard, S., Petrovski, G., & Kolko, M. (2022). The physicochemical properties of preserved and preservative-free latanoprost eye drops and their impact on human conjunctival goblet cells. Acta Ophthalmologica, 100(S267). https://doi.org/10.1111/j.1755-3768.2022.071

Vancouver

Müllertz OAO, Nagstrup AH, Mouhammad ZA, Freiberg JC, Nagymihály R, Jacobsen J et al. The physicochemical properties of preserved and preservative-free latanoprost eye drops and their impact on human conjunctival goblet cells. Acta Ophthalmologica. 2022;100(S267). https://doi.org/10.1111/j.1755-3768.2022.071

Author

Müllertz, Olivia Amanda Oest ; Nagstrup, Anne Hedengran ; Mouhammad, Zaynab Ahmad ; Freiberg, Josefine Clement ; Nagymihály, Richárd ; Jacobsen, Jette ; Larsen, Susan Weng ; Bair, Jeffrey A ; Utheim, Tor Paaske ; Dartt, Darlene A. ; Heegaard, Steffen ; Petrovski, Goran ; Kolko, Miriam. / The physicochemical properties of preserved and preservative-free latanoprost eye drops and their impact on human conjunctival goblet cells. In: Acta Ophthalmologica. 2022 ; Vol. 100, No. S267.

Bibtex

@article{66a3436f3939468bbcd03c0ccef6a07b,
title = "The physicochemical properties of preserved and preservative-free latanoprost eye drops and their impact on human conjunctival goblet cells",
abstract = "PurposeTo investigate the impact on human conjunctival goblet cell (GC) survival and mucin release for benzalkonium chloride (BAK) preserved and preservative-free (PF) 0.005% (w/v) latanoprost (LT) eye drops, and to compare the eye drops{\textquoteright} physicochemical properties.MethodsGC cultures were established from human conjunctival donor tissue. The impact of eye drops on GC survival was assessed using lactate dehydrogenase (LDH) and tetrazolium dye (MTT) colorimetric assays. Mucin release was evaluated through mucin-specific immunostaining. pH-value, osmolality, drop mass and surface tension for all LT eye drops were measured.ResultsAfter application with PF-LT for 30 minutes (min), the GC survival was maintained (p = 0.7043), while all BAK-LT eye drops reduced survival to approximately 70% (p<0.0001). Following application with PF-LT for 30 min, mucin was found around the GC nucleus, as seen in the vehicle control, indicating no secretion. In contrast, BAK-LT caused diffuse staining of mucin, similar to the secretagogue histamine, indicating stimulation of secretion. The pH of the BAK-LT and PF-LT eye drops were 6.0-6.9 and 6.8, respectively. The osmolality was 258-288 mOsm/kg for the BAK-LT eye drops and 276 for PF-LT eye drop. The mean drop mass was 26-31 mg for the BAK-LT eye drops and 30mg for PF-LT. The surface tension was lower for all BAK-LT eye drops (31.1-32.1 mN/m) compared to PF-LT (42 mN/m).ConclusionsPF-LT compared to various branded and generic LT preparations containing BAK should be used to improve goblet cell tolerability and ocular surface health.",
author = "M{\"u}llertz, {Olivia Amanda Oest} and Nagstrup, {Anne Hedengran} and Mouhammad, {Zaynab Ahmad} and Freiberg, {Josefine Clement} and Rich{\'a}rd Nagymih{\'a}ly and Jette Jacobsen and Larsen, {Susan Weng} and Bair, {Jeffrey A} and Utheim, {Tor Paaske} and Dartt, {Darlene A.} and Steffen Heegaard and Goran Petrovski and Miriam Kolko",
note = "Special Issue: Abstracts from the 2021 European Association for Vision and Eye Research Festival; European Association for Vision and Eye Research Festival (EVER) 2021 ; Conference date: 02-10-2021 Through 02-10-2021",
year = "2022",
doi = "10.1111/j.1755-3768.2022.071",
language = "English",
volume = "100",
journal = "Acta Ophthalmologica",
issn = "1755-375X",
publisher = "Wiley-Blackwell",
number = "S267",

}

RIS

TY - ABST

T1 - The physicochemical properties of preserved and preservative-free latanoprost eye drops and their impact on human conjunctival goblet cells

AU - Müllertz, Olivia Amanda Oest

AU - Nagstrup, Anne Hedengran

AU - Mouhammad, Zaynab Ahmad

AU - Freiberg, Josefine Clement

AU - Nagymihály, Richárd

AU - Jacobsen, Jette

AU - Larsen, Susan Weng

AU - Bair, Jeffrey A

AU - Utheim, Tor Paaske

AU - Dartt, Darlene A.

AU - Heegaard, Steffen

AU - Petrovski, Goran

AU - Kolko, Miriam

N1 - Special Issue: Abstracts from the 2021 European Association for Vision and Eye Research Festival

PY - 2022

Y1 - 2022

N2 - PurposeTo investigate the impact on human conjunctival goblet cell (GC) survival and mucin release for benzalkonium chloride (BAK) preserved and preservative-free (PF) 0.005% (w/v) latanoprost (LT) eye drops, and to compare the eye drops’ physicochemical properties.MethodsGC cultures were established from human conjunctival donor tissue. The impact of eye drops on GC survival was assessed using lactate dehydrogenase (LDH) and tetrazolium dye (MTT) colorimetric assays. Mucin release was evaluated through mucin-specific immunostaining. pH-value, osmolality, drop mass and surface tension for all LT eye drops were measured.ResultsAfter application with PF-LT for 30 minutes (min), the GC survival was maintained (p = 0.7043), while all BAK-LT eye drops reduced survival to approximately 70% (p<0.0001). Following application with PF-LT for 30 min, mucin was found around the GC nucleus, as seen in the vehicle control, indicating no secretion. In contrast, BAK-LT caused diffuse staining of mucin, similar to the secretagogue histamine, indicating stimulation of secretion. The pH of the BAK-LT and PF-LT eye drops were 6.0-6.9 and 6.8, respectively. The osmolality was 258-288 mOsm/kg for the BAK-LT eye drops and 276 for PF-LT eye drop. The mean drop mass was 26-31 mg for the BAK-LT eye drops and 30mg for PF-LT. The surface tension was lower for all BAK-LT eye drops (31.1-32.1 mN/m) compared to PF-LT (42 mN/m).ConclusionsPF-LT compared to various branded and generic LT preparations containing BAK should be used to improve goblet cell tolerability and ocular surface health.

AB - PurposeTo investigate the impact on human conjunctival goblet cell (GC) survival and mucin release for benzalkonium chloride (BAK) preserved and preservative-free (PF) 0.005% (w/v) latanoprost (LT) eye drops, and to compare the eye drops’ physicochemical properties.MethodsGC cultures were established from human conjunctival donor tissue. The impact of eye drops on GC survival was assessed using lactate dehydrogenase (LDH) and tetrazolium dye (MTT) colorimetric assays. Mucin release was evaluated through mucin-specific immunostaining. pH-value, osmolality, drop mass and surface tension for all LT eye drops were measured.ResultsAfter application with PF-LT for 30 minutes (min), the GC survival was maintained (p = 0.7043), while all BAK-LT eye drops reduced survival to approximately 70% (p<0.0001). Following application with PF-LT for 30 min, mucin was found around the GC nucleus, as seen in the vehicle control, indicating no secretion. In contrast, BAK-LT caused diffuse staining of mucin, similar to the secretagogue histamine, indicating stimulation of secretion. The pH of the BAK-LT and PF-LT eye drops were 6.0-6.9 and 6.8, respectively. The osmolality was 258-288 mOsm/kg for the BAK-LT eye drops and 276 for PF-LT eye drop. The mean drop mass was 26-31 mg for the BAK-LT eye drops and 30mg for PF-LT. The surface tension was lower for all BAK-LT eye drops (31.1-32.1 mN/m) compared to PF-LT (42 mN/m).ConclusionsPF-LT compared to various branded and generic LT preparations containing BAK should be used to improve goblet cell tolerability and ocular surface health.

U2 - 10.1111/j.1755-3768.2022.071

DO - 10.1111/j.1755-3768.2022.071

M3 - Conference abstract in journal

VL - 100

JO - Acta Ophthalmologica

JF - Acta Ophthalmologica

SN - 1755-375X

IS - S267

T2 - European Association for Vision and Eye Research Festival (EVER) 2021

Y2 - 2 October 2021 through 2 October 2021

ER -

ID: 297348901