Systematic investigation of the role of surfactant composition and choice of oil: Design of a nanoemulsion-based adjuvant inducing concomitant humoral and CD4+ T-cell responses

Research output: Contribution to journalJournal articlepeer-review

Standard

Systematic investigation of the role of surfactant composition and choice of oil : Design of a nanoemulsion-based adjuvant inducing concomitant humoral and CD4+ T-cell responses. / Schmidt, Signe Tandrup; Neustrup, Malene Aaby; Harloff-Helleberg, Stine; Korsholm, Karen Smith; Rades, Thomas; Andersen, Peter; Christensen, Dennis; Foged, Camilla.

In: Pharmaceutical Research, Vol. 34, No. 8, 08.2017, p. 1716-1727.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Schmidt, ST, Neustrup, MA, Harloff-Helleberg, S, Korsholm, KS, Rades, T, Andersen, P, Christensen, D & Foged, C 2017, 'Systematic investigation of the role of surfactant composition and choice of oil: Design of a nanoemulsion-based adjuvant inducing concomitant humoral and CD4+ T-cell responses', Pharmaceutical Research, vol. 34, no. 8, pp. 1716-1727. https://doi.org/10.1007/s11095-017-2180-9

APA

Schmidt, S. T., Neustrup, M. A., Harloff-Helleberg, S., Korsholm, K. S., Rades, T., Andersen, P., Christensen, D., & Foged, C. (2017). Systematic investigation of the role of surfactant composition and choice of oil: Design of a nanoemulsion-based adjuvant inducing concomitant humoral and CD4+ T-cell responses. Pharmaceutical Research, 34(8), 1716-1727. https://doi.org/10.1007/s11095-017-2180-9

Vancouver

Schmidt ST, Neustrup MA, Harloff-Helleberg S, Korsholm KS, Rades T, Andersen P et al. Systematic investigation of the role of surfactant composition and choice of oil: Design of a nanoemulsion-based adjuvant inducing concomitant humoral and CD4+ T-cell responses. Pharmaceutical Research. 2017 Aug;34(8):1716-1727. https://doi.org/10.1007/s11095-017-2180-9

Author

Schmidt, Signe Tandrup ; Neustrup, Malene Aaby ; Harloff-Helleberg, Stine ; Korsholm, Karen Smith ; Rades, Thomas ; Andersen, Peter ; Christensen, Dennis ; Foged, Camilla. / Systematic investigation of the role of surfactant composition and choice of oil : Design of a nanoemulsion-based adjuvant inducing concomitant humoral and CD4+ T-cell responses. In: Pharmaceutical Research. 2017 ; Vol. 34, No. 8. pp. 1716-1727.

Bibtex

@article{6c03ceefa5414977847a78396707b012,
title = "Systematic investigation of the role of surfactant composition and choice of oil: Design of a nanoemulsion-based adjuvant inducing concomitant humoral and CD4+ T-cell responses",
abstract = "PURPOSE:Induction of cell-mediated immune (CMI) responses is crucial for vaccine-mediated protection against difficult vaccine targets, e.g., Chlamydia trachomatis (Ct). Adjuvants are included in subunit vaccines to potentiate immune responses, but many marketed adjuvants stimulate predominantly humoral immune responses. Therefore, there is an unmet medical need for new adjuvants, which potentiate humoral and CMI responses. The purpose was to design an oil-in-water nanoemulsion adjuvant containing a synthetic CMI-inducing mycobacterial monomycoloyl glycerol (MMG) analogue to concomitantly induce humoral and CMI responses.METHODS:The influence of emulsion composition was analyzed using a systematic approach. Three factors were varied: i) saturation of the oil phase, ii) type and saturation of the applied surfactant mixture, and iii) surfactant mixture net charge.RESULTS:The emulsions were colloidally stable with a droplet diameter of 150-250 nm, and the zeta-potential correlated closely with the net charge of the surfactant mixture. Only cationic emulsions containing the unsaturated surfactant mixture induced concomitant humoral and CMI responses upon immunization of mice with a Ct antigen, and the responses were enhanced when squalene was applied as the oil phase. In contrast, emulsions with neutral and net negative zeta-potentials did not induce CMI responses. The saturation degree of the oil phase did not influence the adjuvanticity.CONCLUSION:Cationic, MMG analogue-containing nanoemulsions are potential adjuvants for vaccines against pathogens for which both humoral and CMI responses are needed.",
author = "Schmidt, {Signe Tandrup} and Neustrup, {Malene Aaby} and Stine Harloff-Helleberg and Korsholm, {Karen Smith} and Thomas Rades and Peter Andersen and Dennis Christensen and Camilla Foged",
year = "2017",
month = aug,
doi = "10.1007/s11095-017-2180-9",
language = "English",
volume = "34",
pages = "1716--1727",
journal = "Pharmaceutical Research",
issn = "0724-8741",
publisher = "Springer",
number = "8",

}

RIS

TY - JOUR

T1 - Systematic investigation of the role of surfactant composition and choice of oil

T2 - Design of a nanoemulsion-based adjuvant inducing concomitant humoral and CD4+ T-cell responses

AU - Schmidt, Signe Tandrup

AU - Neustrup, Malene Aaby

AU - Harloff-Helleberg, Stine

AU - Korsholm, Karen Smith

AU - Rades, Thomas

AU - Andersen, Peter

AU - Christensen, Dennis

AU - Foged, Camilla

PY - 2017/8

Y1 - 2017/8

N2 - PURPOSE:Induction of cell-mediated immune (CMI) responses is crucial for vaccine-mediated protection against difficult vaccine targets, e.g., Chlamydia trachomatis (Ct). Adjuvants are included in subunit vaccines to potentiate immune responses, but many marketed adjuvants stimulate predominantly humoral immune responses. Therefore, there is an unmet medical need for new adjuvants, which potentiate humoral and CMI responses. The purpose was to design an oil-in-water nanoemulsion adjuvant containing a synthetic CMI-inducing mycobacterial monomycoloyl glycerol (MMG) analogue to concomitantly induce humoral and CMI responses.METHODS:The influence of emulsion composition was analyzed using a systematic approach. Three factors were varied: i) saturation of the oil phase, ii) type and saturation of the applied surfactant mixture, and iii) surfactant mixture net charge.RESULTS:The emulsions were colloidally stable with a droplet diameter of 150-250 nm, and the zeta-potential correlated closely with the net charge of the surfactant mixture. Only cationic emulsions containing the unsaturated surfactant mixture induced concomitant humoral and CMI responses upon immunization of mice with a Ct antigen, and the responses were enhanced when squalene was applied as the oil phase. In contrast, emulsions with neutral and net negative zeta-potentials did not induce CMI responses. The saturation degree of the oil phase did not influence the adjuvanticity.CONCLUSION:Cationic, MMG analogue-containing nanoemulsions are potential adjuvants for vaccines against pathogens for which both humoral and CMI responses are needed.

AB - PURPOSE:Induction of cell-mediated immune (CMI) responses is crucial for vaccine-mediated protection against difficult vaccine targets, e.g., Chlamydia trachomatis (Ct). Adjuvants are included in subunit vaccines to potentiate immune responses, but many marketed adjuvants stimulate predominantly humoral immune responses. Therefore, there is an unmet medical need for new adjuvants, which potentiate humoral and CMI responses. The purpose was to design an oil-in-water nanoemulsion adjuvant containing a synthetic CMI-inducing mycobacterial monomycoloyl glycerol (MMG) analogue to concomitantly induce humoral and CMI responses.METHODS:The influence of emulsion composition was analyzed using a systematic approach. Three factors were varied: i) saturation of the oil phase, ii) type and saturation of the applied surfactant mixture, and iii) surfactant mixture net charge.RESULTS:The emulsions were colloidally stable with a droplet diameter of 150-250 nm, and the zeta-potential correlated closely with the net charge of the surfactant mixture. Only cationic emulsions containing the unsaturated surfactant mixture induced concomitant humoral and CMI responses upon immunization of mice with a Ct antigen, and the responses were enhanced when squalene was applied as the oil phase. In contrast, emulsions with neutral and net negative zeta-potentials did not induce CMI responses. The saturation degree of the oil phase did not influence the adjuvanticity.CONCLUSION:Cationic, MMG analogue-containing nanoemulsions are potential adjuvants for vaccines against pathogens for which both humoral and CMI responses are needed.

U2 - 10.1007/s11095-017-2180-9

DO - 10.1007/s11095-017-2180-9

M3 - Journal article

C2 - 28516400

VL - 34

SP - 1716

EP - 1727

JO - Pharmaceutical Research

JF - Pharmaceutical Research

SN - 0724-8741

IS - 8

ER -

ID: 177413302