Synthesis, isolation and identification of glucuronides and mercapturic acids of a novel antiparasitic agent, licochalcone A

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Synthesis, isolation and identification of glucuronides and mercapturic acids of a novel antiparasitic agent, licochalcone A. / Nadelmann, L.; Tjornelund, J.; Hansen, S. H.; Cornett, Claus; Sidelmann, U. G.; Braumann, U.; Christensen, E.; Christensen, S. B.

In: Xenobiotica, Vol. 27, No. 7, 1997, p. 667-680.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Nadelmann, L, Tjornelund, J, Hansen, SH, Cornett, C, Sidelmann, UG, Braumann, U, Christensen, E & Christensen, SB 1997, 'Synthesis, isolation and identification of glucuronides and mercapturic acids of a novel antiparasitic agent, licochalcone A', Xenobiotica, vol. 27, no. 7, pp. 667-680.

APA

Nadelmann, L., Tjornelund, J., Hansen, S. H., Cornett, C., Sidelmann, U. G., Braumann, U., ... Christensen, S. B. (1997). Synthesis, isolation and identification of glucuronides and mercapturic acids of a novel antiparasitic agent, licochalcone A. Xenobiotica, 27(7), 667-680.

Vancouver

Nadelmann L, Tjornelund J, Hansen SH, Cornett C, Sidelmann UG, Braumann U et al. Synthesis, isolation and identification of glucuronides and mercapturic acids of a novel antiparasitic agent, licochalcone A. Xenobiotica. 1997;27(7):667-680.

Author

Nadelmann, L. ; Tjornelund, J. ; Hansen, S. H. ; Cornett, Claus ; Sidelmann, U. G. ; Braumann, U. ; Christensen, E. ; Christensen, S. B. / Synthesis, isolation and identification of glucuronides and mercapturic acids of a novel antiparasitic agent, licochalcone A. In: Xenobiotica. 1997 ; Vol. 27, No. 7. pp. 667-680.

Bibtex

@article{bfc7a04fa4204650a938d6dc0b3626c9,
title = "Synthesis, isolation and identification of glucuronides and mercapturic acids of a novel antiparasitic agent, licochalcone A",
abstract = "1. Four glucuronic acid conjugates of licochalcone A (Lica), and their metabolites, have been synthesized using rabbit and pig liver microsomes and purified by preparative hplc. 2. The glucuronides were identified as E-Lica 4'-O-beta-glucuronide, E and Z-Lica 4-O-beta-glucuronide and a mono-glucuronide conjugate of a beta-hydroxylated Lica metabolite. The metabolites were identified by hplc-nmr (one and two-dimensional nmr) as well as hplc-ms. 3. At pH 8.5 Lica reacted with N-acetyl-L-cysteine giving the two epimeric conjugates, which were then isolated by preparative hplc and identified by one and two-dimensional nmr spectroscopic methods. 4. Only two glucuronic acid conjugates (E- and Z-Lica 4-O-beta-glucuronide) were found in the urine of rat after i.p. administration of a single dose of Lica.",
keywords = "performance liquid-chromatography magnetic-resonance spectroscopy dynamically modified silica h-1-nmr spectroscopy glutathione adduct metabolites urine leishmania ibuprofen mice",
author = "L. Nadelmann and J. Tjornelund and Hansen, {S. H.} and Claus Cornett and Sidelmann, {U. G.} and U. Braumann and E. Christensen and Christensen, {S. B.}",
year = "1997",
language = "Udefineret/Ukendt",
volume = "27",
pages = "667--680",
journal = "Xenobiotica",
issn = "0049-8254",
publisher = "Taylor & Francis",
number = "7",

}

RIS

TY - JOUR

T1 - Synthesis, isolation and identification of glucuronides and mercapturic acids of a novel antiparasitic agent, licochalcone A

AU - Nadelmann, L.

AU - Tjornelund, J.

AU - Hansen, S. H.

AU - Cornett, Claus

AU - Sidelmann, U. G.

AU - Braumann, U.

AU - Christensen, E.

AU - Christensen, S. B.

PY - 1997

Y1 - 1997

N2 - 1. Four glucuronic acid conjugates of licochalcone A (Lica), and their metabolites, have been synthesized using rabbit and pig liver microsomes and purified by preparative hplc. 2. The glucuronides were identified as E-Lica 4'-O-beta-glucuronide, E and Z-Lica 4-O-beta-glucuronide and a mono-glucuronide conjugate of a beta-hydroxylated Lica metabolite. The metabolites were identified by hplc-nmr (one and two-dimensional nmr) as well as hplc-ms. 3. At pH 8.5 Lica reacted with N-acetyl-L-cysteine giving the two epimeric conjugates, which were then isolated by preparative hplc and identified by one and two-dimensional nmr spectroscopic methods. 4. Only two glucuronic acid conjugates (E- and Z-Lica 4-O-beta-glucuronide) were found in the urine of rat after i.p. administration of a single dose of Lica.

AB - 1. Four glucuronic acid conjugates of licochalcone A (Lica), and their metabolites, have been synthesized using rabbit and pig liver microsomes and purified by preparative hplc. 2. The glucuronides were identified as E-Lica 4'-O-beta-glucuronide, E and Z-Lica 4-O-beta-glucuronide and a mono-glucuronide conjugate of a beta-hydroxylated Lica metabolite. The metabolites were identified by hplc-nmr (one and two-dimensional nmr) as well as hplc-ms. 3. At pH 8.5 Lica reacted with N-acetyl-L-cysteine giving the two epimeric conjugates, which were then isolated by preparative hplc and identified by one and two-dimensional nmr spectroscopic methods. 4. Only two glucuronic acid conjugates (E- and Z-Lica 4-O-beta-glucuronide) were found in the urine of rat after i.p. administration of a single dose of Lica.

KW - performance liquid-chromatography magnetic-resonance spectroscopy dynamically modified silica h-1-nmr spectroscopy glutathione adduct metabolites urine leishmania ibuprofen mice

M3 - Tidsskriftartikel

VL - 27

SP - 667

EP - 680

JO - Xenobiotica

JF - Xenobiotica

SN - 0049-8254

IS - 7

ER -

ID: 38061951