Swelling lecithin: cholesterol implants for the controlled release of proteins

Research output: Contribution to journalJournal articlepeer-review

Standard

Swelling lecithin : cholesterol implants for the controlled release of proteins. / Tantipolphan, Ruedeeporn; Rades, Thomas; Medlicott, Natalie J.

In: Journal of Liposome Research, Vol. 19, No. 1, 2009, p. 37-48.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Tantipolphan, R, Rades, T & Medlicott, NJ 2009, 'Swelling lecithin: cholesterol implants for the controlled release of proteins', Journal of Liposome Research, vol. 19, no. 1, pp. 37-48. https://doi.org/10.1080/08982100802636434

APA

Tantipolphan, R., Rades, T., & Medlicott, N. J. (2009). Swelling lecithin: cholesterol implants for the controlled release of proteins. Journal of Liposome Research, 19(1), 37-48. https://doi.org/10.1080/08982100802636434

Vancouver

Tantipolphan R, Rades T, Medlicott NJ. Swelling lecithin: cholesterol implants for the controlled release of proteins. Journal of Liposome Research. 2009;19(1):37-48. https://doi.org/10.1080/08982100802636434

Author

Tantipolphan, Ruedeeporn ; Rades, Thomas ; Medlicott, Natalie J. / Swelling lecithin : cholesterol implants for the controlled release of proteins. In: Journal of Liposome Research. 2009 ; Vol. 19, No. 1. pp. 37-48.

Bibtex

@article{f081edd296fc420983aa3a11eaace03a,
title = "Swelling lecithin: cholesterol implants for the controlled release of proteins",
abstract = "This work demonstrated the effect of two salts as potential simple formulation excipients in modifying hydration properties, phase behavior, and protein release from lecithin-based implants. In vitro release of a model protein, bovine serum albumin (BSA), from cylindrical-shaped lecithin and lecithin:cholesterol (1:1 w/w) implants containing 0, 10, or 30% w/w NaCl or CaCl2 was studied. In the absence of salts, BSA was released from lecithin and lecithin:cholesterol implants with a high monomer content and the release profiles were similar to those previously reported. Cholesterol increased the swelling, induced the formation of myelin structures, and reduced BSA release from the matrices. Addition of the salts to lecithin:cholesterol implants further enhanced the swelling, altered the hydrated morphology, and inhibited protein release. Analyses showed that BSA associated into multimers within these swollen lipid matrices but retained a high degree of protein native structure. Factors that may have contributed to the inhibition of the in vitro release included 1) the swollen multilamellar layers assembled as diffusional barriers, 2) adsorption of BSA onto the hydrated lipid vesicles, and 3) formation of protein aggregates.",
author = "Ruedeeporn Tantipolphan and Thomas Rades and Medlicott, {Natalie J}",
year = "2009",
doi = "10.1080/08982100802636434",
language = "English",
volume = "19",
pages = "37--48",
journal = "Journal of Liposome Research",
issn = "0898-2104",
publisher = "Taylor & Francis",
number = "1",

}

RIS

TY - JOUR

T1 - Swelling lecithin

T2 - cholesterol implants for the controlled release of proteins

AU - Tantipolphan, Ruedeeporn

AU - Rades, Thomas

AU - Medlicott, Natalie J

PY - 2009

Y1 - 2009

N2 - This work demonstrated the effect of two salts as potential simple formulation excipients in modifying hydration properties, phase behavior, and protein release from lecithin-based implants. In vitro release of a model protein, bovine serum albumin (BSA), from cylindrical-shaped lecithin and lecithin:cholesterol (1:1 w/w) implants containing 0, 10, or 30% w/w NaCl or CaCl2 was studied. In the absence of salts, BSA was released from lecithin and lecithin:cholesterol implants with a high monomer content and the release profiles were similar to those previously reported. Cholesterol increased the swelling, induced the formation of myelin structures, and reduced BSA release from the matrices. Addition of the salts to lecithin:cholesterol implants further enhanced the swelling, altered the hydrated morphology, and inhibited protein release. Analyses showed that BSA associated into multimers within these swollen lipid matrices but retained a high degree of protein native structure. Factors that may have contributed to the inhibition of the in vitro release included 1) the swollen multilamellar layers assembled as diffusional barriers, 2) adsorption of BSA onto the hydrated lipid vesicles, and 3) formation of protein aggregates.

AB - This work demonstrated the effect of two salts as potential simple formulation excipients in modifying hydration properties, phase behavior, and protein release from lecithin-based implants. In vitro release of a model protein, bovine serum albumin (BSA), from cylindrical-shaped lecithin and lecithin:cholesterol (1:1 w/w) implants containing 0, 10, or 30% w/w NaCl or CaCl2 was studied. In the absence of salts, BSA was released from lecithin and lecithin:cholesterol implants with a high monomer content and the release profiles were similar to those previously reported. Cholesterol increased the swelling, induced the formation of myelin structures, and reduced BSA release from the matrices. Addition of the salts to lecithin:cholesterol implants further enhanced the swelling, altered the hydrated morphology, and inhibited protein release. Analyses showed that BSA associated into multimers within these swollen lipid matrices but retained a high degree of protein native structure. Factors that may have contributed to the inhibition of the in vitro release included 1) the swollen multilamellar layers assembled as diffusional barriers, 2) adsorption of BSA onto the hydrated lipid vesicles, and 3) formation of protein aggregates.

U2 - 10.1080/08982100802636434

DO - 10.1080/08982100802636434

M3 - Journal article

C2 - 19515006

VL - 19

SP - 37

EP - 48

JO - Journal of Liposome Research

JF - Journal of Liposome Research

SN - 0898-2104

IS - 1

ER -

ID: 40349212