Studying furosemide solubilization using an in vitro model simulating gastrointestinal digestion and drug solubilization in neonates and young infants

Research output: Contribution to journalJournal articlepeer-review

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Studying furosemide solubilization using an in vitro model simulating gastrointestinal digestion and drug solubilization in neonates and young infants. / Klitgaard, Mette; Sassene, Philip Jonas; Selen, Arzu; Müllertz, Anette; Berthelsen, Ragna.

In: European Journal of Pharmaceutical Sciences, Vol. 109, 15.11.2017, p. 191-199.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Klitgaard, M, Sassene, PJ, Selen, A, Müllertz, A & Berthelsen, R 2017, 'Studying furosemide solubilization using an in vitro model simulating gastrointestinal digestion and drug solubilization in neonates and young infants', European Journal of Pharmaceutical Sciences, vol. 109, pp. 191-199. https://doi.org/10.1016/j.ejps.2017.08.003

APA

Klitgaard, M., Sassene, P. J., Selen, A., Müllertz, A., & Berthelsen, R. (2017). Studying furosemide solubilization using an in vitro model simulating gastrointestinal digestion and drug solubilization in neonates and young infants. European Journal of Pharmaceutical Sciences, 109, 191-199. https://doi.org/10.1016/j.ejps.2017.08.003

Vancouver

Klitgaard M, Sassene PJ, Selen A, Müllertz A, Berthelsen R. Studying furosemide solubilization using an in vitro model simulating gastrointestinal digestion and drug solubilization in neonates and young infants. European Journal of Pharmaceutical Sciences. 2017 Nov 15;109:191-199. https://doi.org/10.1016/j.ejps.2017.08.003

Author

Klitgaard, Mette ; Sassene, Philip Jonas ; Selen, Arzu ; Müllertz, Anette ; Berthelsen, Ragna. / Studying furosemide solubilization using an in vitro model simulating gastrointestinal digestion and drug solubilization in neonates and young infants. In: European Journal of Pharmaceutical Sciences. 2017 ; Vol. 109. pp. 191-199.

Bibtex

@article{d997dd1b8c06457785d0931ec57e1f7b,
title = "Studying furosemide solubilization using an in vitro model simulating gastrointestinal digestion and drug solubilization in neonates and young infants",
abstract = "OBJECTIVE: The aim of the present study was to study the oral performance of furosemide in neonates and young infants using a newly developed in vitro model simulating digestion and drug solubilization in the gastrointestinal (GI) tract of the human neonate and young infant population (age 0-2months).METHODS: The utilized in vitro model was designed to mimic the digestion and drug solubilization processes occurring in the stomach, and the small intestine of the neonate and young infant population, using physiologically relevant media, volumes and digestive enzymes. Overall the experimental model setup was based on the dynamic in vitro lipolysis model previously described by Fernandez et al. (2009). The amount of furosemide solubilized in the aqueous phase during a digestion study was used as an estimate for the amount of drug available for absorption in vivo. By varying different factors in the model setup, e.g. presence of food (food-effect), effect of digestion (tested with and without addition of digestive enzymes), and properties of the dosage form, it was possible to estimate the importance of these factors in vivo.KEY FINDINGS AND CONCLUSIONS: The present in vitro data suggest that the oral performance of furosemide in neonates and young infants will be increased by the presence of food (frequent feedings) due to increased drug solubilization, however, not influenced by the GI digestion of this food. The properties of the dosage form (immediate release tablets) did not affect the drug solubilization as compared to administration of the pure drug powder.",
keywords = "Journal Article",
author = "Mette Klitgaard and Sassene, {Philip Jonas} and Arzu Selen and Anette M{\"u}llertz and Ragna Berthelsen",
note = "Copyright {\textcopyright} 2017 Elsevier B.V. All rights reserved.",
year = "2017",
month = nov,
day = "15",
doi = "10.1016/j.ejps.2017.08.003",
language = "English",
volume = "109",
pages = "191--199",
journal = "Norvegica Pharmaceutica Acta",
issn = "0928-0987",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Studying furosemide solubilization using an in vitro model simulating gastrointestinal digestion and drug solubilization in neonates and young infants

AU - Klitgaard, Mette

AU - Sassene, Philip Jonas

AU - Selen, Arzu

AU - Müllertz, Anette

AU - Berthelsen, Ragna

N1 - Copyright © 2017 Elsevier B.V. All rights reserved.

PY - 2017/11/15

Y1 - 2017/11/15

N2 - OBJECTIVE: The aim of the present study was to study the oral performance of furosemide in neonates and young infants using a newly developed in vitro model simulating digestion and drug solubilization in the gastrointestinal (GI) tract of the human neonate and young infant population (age 0-2months).METHODS: The utilized in vitro model was designed to mimic the digestion and drug solubilization processes occurring in the stomach, and the small intestine of the neonate and young infant population, using physiologically relevant media, volumes and digestive enzymes. Overall the experimental model setup was based on the dynamic in vitro lipolysis model previously described by Fernandez et al. (2009). The amount of furosemide solubilized in the aqueous phase during a digestion study was used as an estimate for the amount of drug available for absorption in vivo. By varying different factors in the model setup, e.g. presence of food (food-effect), effect of digestion (tested with and without addition of digestive enzymes), and properties of the dosage form, it was possible to estimate the importance of these factors in vivo.KEY FINDINGS AND CONCLUSIONS: The present in vitro data suggest that the oral performance of furosemide in neonates and young infants will be increased by the presence of food (frequent feedings) due to increased drug solubilization, however, not influenced by the GI digestion of this food. The properties of the dosage form (immediate release tablets) did not affect the drug solubilization as compared to administration of the pure drug powder.

AB - OBJECTIVE: The aim of the present study was to study the oral performance of furosemide in neonates and young infants using a newly developed in vitro model simulating digestion and drug solubilization in the gastrointestinal (GI) tract of the human neonate and young infant population (age 0-2months).METHODS: The utilized in vitro model was designed to mimic the digestion and drug solubilization processes occurring in the stomach, and the small intestine of the neonate and young infant population, using physiologically relevant media, volumes and digestive enzymes. Overall the experimental model setup was based on the dynamic in vitro lipolysis model previously described by Fernandez et al. (2009). The amount of furosemide solubilized in the aqueous phase during a digestion study was used as an estimate for the amount of drug available for absorption in vivo. By varying different factors in the model setup, e.g. presence of food (food-effect), effect of digestion (tested with and without addition of digestive enzymes), and properties of the dosage form, it was possible to estimate the importance of these factors in vivo.KEY FINDINGS AND CONCLUSIONS: The present in vitro data suggest that the oral performance of furosemide in neonates and young infants will be increased by the presence of food (frequent feedings) due to increased drug solubilization, however, not influenced by the GI digestion of this food. The properties of the dosage form (immediate release tablets) did not affect the drug solubilization as compared to administration of the pure drug powder.

KW - Journal Article

U2 - 10.1016/j.ejps.2017.08.003

DO - 10.1016/j.ejps.2017.08.003

M3 - Journal article

C2 - 28803922

VL - 109

SP - 191

EP - 199

JO - Norvegica Pharmaceutica Acta

JF - Norvegica Pharmaceutica Acta

SN - 0928-0987

ER -

ID: 185404142