Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation

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Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation. / Bøgh, Marie; García-Díaz, María; Müllertz, Anette; Nielsen, Hanne Mørck.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 94, No. Part A, 09.2015, p. 136–143.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Bøgh, M, García-Díaz, M, Müllertz, A & Nielsen, HM 2015, 'Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation', European Journal of Pharmaceutics and Biopharmaceutics, vol. 94, no. Part A, pp. 136–143. https://doi.org/10.1016/j.ejpb.2015.01.014

APA

Bøgh, M., García-Díaz, M., Müllertz, A., & Nielsen, H. M. (2015). Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation. European Journal of Pharmaceutics and Biopharmaceutics, 94(Part A), 136–143. https://doi.org/10.1016/j.ejpb.2015.01.014

Vancouver

Bøgh M, García-Díaz M, Müllertz A, Nielsen HM. Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation. European Journal of Pharmaceutics and Biopharmaceutics. 2015 Sep;94(Part A):136–143. https://doi.org/10.1016/j.ejpb.2015.01.014

Author

Bøgh, Marie ; García-Díaz, María ; Müllertz, Anette ; Nielsen, Hanne Mørck. / Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation. In: European Journal of Pharmaceutics and Biopharmaceutics. 2015 ; Vol. 94, No. Part A. pp. 136–143.

Bibtex

@article{71f37de5897f4bd1ab6e414c4282c667,
title = "Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation",
abstract = "The mucus lining of the gastrointestinal tract epithelium is recognized as a barrier to efficient oral drug delivery. Recently, a new in vitro model for assessment of drug permeation across intestinal mucosa was established by applying a biosimilar mucus matrix to the surface of Caco-2 cell monolayers. The aim of the present study was to gain more insight into the steric and interactive barrier properties of intestinal mucus by studying the permeation of peptides and model compounds across the biosimilar mucus as well as across porcine intestinal mucus (PIM). As PIM disrupted the Caco-2 cell monolayers, a cell-free mucus barrier model was implemented in the studies. Both the biosimilar mucus and the PIM reduced the permeation of the selected peptide drugs to varying degrees illustrating the interactive properties of both mucus matrices. The reduction in peptide permeation was decreased depending on the cationicity and H-bonding capacity of the permeant clearly demonstrated by using the biosimilar mucus, whereas the larger inter sample variation of the PIM matrix obstructed similarly clear conclusions. Thus, for mechanistic studies of permeation across mucus and mucosa the biosimilar mucus offers a relevant and reproducible alternative to native mucus.",
author = "Marie B{\o}gh and Mar{\'i}a Garc{\'i}a-D{\'i}az and Anette M{\"u}llertz and Nielsen, {Hanne M{\o}rck}",
note = "Copyright {\circledC} 2015. Published by Elsevier B.V.",
year = "2015",
month = "9",
doi = "10.1016/j.ejpb.2015.01.014",
language = "English",
volume = "94",
pages = "136–143",
journal = "European Journal of Pharmaceutics and Biopharmaceutics",
issn = "0939-6411",
publisher = "Elsevier",
number = "Part A",

}

RIS

TY - JOUR

T1 - Steric and interactive barrier properties of intestinal mucus elucidated by particle diffusion and peptide permeation

AU - Bøgh, Marie

AU - García-Díaz, María

AU - Müllertz, Anette

AU - Nielsen, Hanne Mørck

N1 - Copyright © 2015. Published by Elsevier B.V.

PY - 2015/9

Y1 - 2015/9

N2 - The mucus lining of the gastrointestinal tract epithelium is recognized as a barrier to efficient oral drug delivery. Recently, a new in vitro model for assessment of drug permeation across intestinal mucosa was established by applying a biosimilar mucus matrix to the surface of Caco-2 cell monolayers. The aim of the present study was to gain more insight into the steric and interactive barrier properties of intestinal mucus by studying the permeation of peptides and model compounds across the biosimilar mucus as well as across porcine intestinal mucus (PIM). As PIM disrupted the Caco-2 cell monolayers, a cell-free mucus barrier model was implemented in the studies. Both the biosimilar mucus and the PIM reduced the permeation of the selected peptide drugs to varying degrees illustrating the interactive properties of both mucus matrices. The reduction in peptide permeation was decreased depending on the cationicity and H-bonding capacity of the permeant clearly demonstrated by using the biosimilar mucus, whereas the larger inter sample variation of the PIM matrix obstructed similarly clear conclusions. Thus, for mechanistic studies of permeation across mucus and mucosa the biosimilar mucus offers a relevant and reproducible alternative to native mucus.

AB - The mucus lining of the gastrointestinal tract epithelium is recognized as a barrier to efficient oral drug delivery. Recently, a new in vitro model for assessment of drug permeation across intestinal mucosa was established by applying a biosimilar mucus matrix to the surface of Caco-2 cell monolayers. The aim of the present study was to gain more insight into the steric and interactive barrier properties of intestinal mucus by studying the permeation of peptides and model compounds across the biosimilar mucus as well as across porcine intestinal mucus (PIM). As PIM disrupted the Caco-2 cell monolayers, a cell-free mucus barrier model was implemented in the studies. Both the biosimilar mucus and the PIM reduced the permeation of the selected peptide drugs to varying degrees illustrating the interactive properties of both mucus matrices. The reduction in peptide permeation was decreased depending on the cationicity and H-bonding capacity of the permeant clearly demonstrated by using the biosimilar mucus, whereas the larger inter sample variation of the PIM matrix obstructed similarly clear conclusions. Thus, for mechanistic studies of permeation across mucus and mucosa the biosimilar mucus offers a relevant and reproducible alternative to native mucus.

U2 - 10.1016/j.ejpb.2015.01.014

DO - 10.1016/j.ejpb.2015.01.014

M3 - Journal article

C2 - 25622791

VL - 94

SP - 136

EP - 143

JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

SN - 0939-6411

IS - Part A

ER -

ID: 131618686