Purification, characterization and ELISA detection of mink immunoglobulins

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Purification, characterization and ELISA detection of mink immunoglobulins. / Martel, Cyril Jean-Marie; Aasted, Bent.

In: Scientifur, Vol. 32, No. 1, 2008, p. 33-40.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Martel, CJ-M & Aasted, B 2008, 'Purification, characterization and ELISA detection of mink immunoglobulins', Scientifur, vol. 32, no. 1, pp. 33-40.

APA

Martel, C. J-M., & Aasted, B. (2008). Purification, characterization and ELISA detection of mink immunoglobulins. Scientifur, 32(1), 33-40.

Vancouver

Martel CJ-M, Aasted B. Purification, characterization and ELISA detection of mink immunoglobulins. Scientifur. 2008;32(1):33-40.

Author

Martel, Cyril Jean-Marie ; Aasted, Bent. / Purification, characterization and ELISA detection of mink immunoglobulins. In: Scientifur. 2008 ; Vol. 32, No. 1. pp. 33-40.

Bibtex

@article{e5d303c0a65711ddb5e9000ea68e967b,
title = "Purification, characterization and ELISA detection of mink immunoglobulins",
abstract = "This study describes easy purification methods for mink IgG, IgA and IgM immunoglobulins. IgG and IgM were purified from normal mink serum, while IgA was purified from mink bile from healthy animals. By SDS-polyacrylamid-gel-electrophoresis  (SDS-PAGE) and immunoblotting under reducing conditions the estimated molecular weights of the immunoglobulin gamma, alpha and mu heavy chains were found to be 54 kDa, 69 kDa and 83 kDa respectively. The purities of purified IgG, IgM and IgA were estimated by immunoglobulin class specific ELISAs to be more than 90% for IgG and IgM, and more than 80% for IgA.",
keywords = "???Dyr som modeller for sygdomme hos mennesker???",
author = "Martel, {Cyril Jean-Marie} and Bent Aasted",
year = "2008",
language = "English",
volume = "32",
pages = "33--40",
journal = "Scientifur",
issn = "0105-2403",
publisher = "International Fur Animal Scientific Association",
number = "1",

}

RIS

TY - JOUR

T1 - Purification, characterization and ELISA detection of mink immunoglobulins

AU - Martel, Cyril Jean-Marie

AU - Aasted, Bent

PY - 2008

Y1 - 2008

N2 - This study describes easy purification methods for mink IgG, IgA and IgM immunoglobulins. IgG and IgM were purified from normal mink serum, while IgA was purified from mink bile from healthy animals. By SDS-polyacrylamid-gel-electrophoresis  (SDS-PAGE) and immunoblotting under reducing conditions the estimated molecular weights of the immunoglobulin gamma, alpha and mu heavy chains were found to be 54 kDa, 69 kDa and 83 kDa respectively. The purities of purified IgG, IgM and IgA were estimated by immunoglobulin class specific ELISAs to be more than 90% for IgG and IgM, and more than 80% for IgA.

AB - This study describes easy purification methods for mink IgG, IgA and IgM immunoglobulins. IgG and IgM were purified from normal mink serum, while IgA was purified from mink bile from healthy animals. By SDS-polyacrylamid-gel-electrophoresis  (SDS-PAGE) and immunoblotting under reducing conditions the estimated molecular weights of the immunoglobulin gamma, alpha and mu heavy chains were found to be 54 kDa, 69 kDa and 83 kDa respectively. The purities of purified IgG, IgM and IgA were estimated by immunoglobulin class specific ELISAs to be more than 90% for IgG and IgM, and more than 80% for IgA.

KW - ???Dyr som modeller for sygdomme hos mennesker???

M3 - Journal article

VL - 32

SP - 33

EP - 40

JO - Scientifur

JF - Scientifur

SN - 0105-2403

IS - 1

ER -

ID: 8297148