Preparation of phytantriol cubosomes by solvent precursor dilution for the delivery of protein vaccines

Research output: Contribution to journalJournal articlepeer-review

Standard

Preparation of phytantriol cubosomes by solvent precursor dilution for the delivery of protein vaccines. / Rizwan, S. B.; Assmus, D.; Boehnke, A.; Hanley, T.; Boyd, B. J.; Rades, T.; Hook, S.

In: European Journal of Pharmaceutics and Biopharmaceutics, Vol. 79, No. 1, 09.2011, p. 15-22.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Rizwan, SB, Assmus, D, Boehnke, A, Hanley, T, Boyd, BJ, Rades, T & Hook, S 2011, 'Preparation of phytantriol cubosomes by solvent precursor dilution for the delivery of protein vaccines', European Journal of Pharmaceutics and Biopharmaceutics, vol. 79, no. 1, pp. 15-22. https://doi.org/10.1016/j.ejpb.2010.12.034

APA

Rizwan, S. B., Assmus, D., Boehnke, A., Hanley, T., Boyd, B. J., Rades, T., & Hook, S. (2011). Preparation of phytantriol cubosomes by solvent precursor dilution for the delivery of protein vaccines. European Journal of Pharmaceutics and Biopharmaceutics, 79(1), 15-22. https://doi.org/10.1016/j.ejpb.2010.12.034

Vancouver

Rizwan SB, Assmus D, Boehnke A, Hanley T, Boyd BJ, Rades T et al. Preparation of phytantriol cubosomes by solvent precursor dilution for the delivery of protein vaccines. European Journal of Pharmaceutics and Biopharmaceutics. 2011 Sep;79(1):15-22. https://doi.org/10.1016/j.ejpb.2010.12.034

Author

Rizwan, S. B. ; Assmus, D. ; Boehnke, A. ; Hanley, T. ; Boyd, B. J. ; Rades, T. ; Hook, S. / Preparation of phytantriol cubosomes by solvent precursor dilution for the delivery of protein vaccines. In: European Journal of Pharmaceutics and Biopharmaceutics. 2011 ; Vol. 79, No. 1. pp. 15-22.

Bibtex

@article{6cc6471204be4365a21c10fdd982354c,
title = "Preparation of phytantriol cubosomes by solvent precursor dilution for the delivery of protein vaccines",
abstract = "Different delivery strategies to improve the immunogenicity of peptide/protein-based vaccines are currently under investigation. In this study, the preparation and physicochemical characterisation of cubosomes, a novel lipid-based particulate system currently being explored for vaccine delivery, was investigated. Cubosomes were prepared from a liquid precursor mixture containing phytantriol or glycerylmonooleate (GMO), F127 for particle stabilisation, and a hydrotrope (ethanol or polyethylene glycol (PEG 200) or propylene glycol (PG)). Several liquid precursors were prepared, and the effect of varying the concentrations of F127 and the hydrotrope on cubosome formation was investigated. Formulations were prepared by fragmentation for comparison. The model protein ovalbumin (Ova) was also entrapped within selected formulations. Submicron-sized particles (180-300 nm) were formed spontaneously upon dilution of the liquid precursors, circumventing the need for the preformed cubic phase used in traditional fragmentation-based methods. The nanostructure of the phytantriol dispersions was determined to be cubic phase using SAXS whilst GMO dispersions had a reverse hexagonal nanostructure coexisting with cubic phase. The greatest entrapment of Ova was within phytantriol cubosomes prepared from liquid precursors. Release of Ova from the various formulations was sustained; however, release was significantly faster and the extent of release was greater from fragmented dispersions compared to liquid precursor formulations. Taken together, these results suggest that phytantriol cubosomes can be prepared using liquid precursors and that it is a suitable alternative to GMO. Furthermore, the high entrapment and the slow release of Ova in vitro highlight the potential of phytantriol cubosomes prepared using liquid precursors as a novel vaccine delivery system.",
keywords = "Cubosomes, Glyceryl monooleate, Liquid precursor, Phytantriol, Proteins, Vaccines",
author = "Rizwan, {S. B.} and D. Assmus and A. Boehnke and T. Hanley and Boyd, {B. J.} and T. Rades and S. Hook",
note = "Funding Information: The authors would like to thank the University of Otago and the School of Pharmacy for providing financial assistance to S R and the Australian Institute of Nuclear Science and Engineering for funding the SAXS studies in this manuscript under grant AINGRA07016.",
year = "2011",
month = sep,
doi = "10.1016/j.ejpb.2010.12.034",
language = "English",
volume = "79",
pages = "15--22",
journal = "European Journal of Pharmaceutics and Biopharmaceutics",
issn = "0939-6411",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Preparation of phytantriol cubosomes by solvent precursor dilution for the delivery of protein vaccines

AU - Rizwan, S. B.

AU - Assmus, D.

AU - Boehnke, A.

AU - Hanley, T.

AU - Boyd, B. J.

AU - Rades, T.

AU - Hook, S.

N1 - Funding Information: The authors would like to thank the University of Otago and the School of Pharmacy for providing financial assistance to S R and the Australian Institute of Nuclear Science and Engineering for funding the SAXS studies in this manuscript under grant AINGRA07016.

PY - 2011/9

Y1 - 2011/9

N2 - Different delivery strategies to improve the immunogenicity of peptide/protein-based vaccines are currently under investigation. In this study, the preparation and physicochemical characterisation of cubosomes, a novel lipid-based particulate system currently being explored for vaccine delivery, was investigated. Cubosomes were prepared from a liquid precursor mixture containing phytantriol or glycerylmonooleate (GMO), F127 for particle stabilisation, and a hydrotrope (ethanol or polyethylene glycol (PEG 200) or propylene glycol (PG)). Several liquid precursors were prepared, and the effect of varying the concentrations of F127 and the hydrotrope on cubosome formation was investigated. Formulations were prepared by fragmentation for comparison. The model protein ovalbumin (Ova) was also entrapped within selected formulations. Submicron-sized particles (180-300 nm) were formed spontaneously upon dilution of the liquid precursors, circumventing the need for the preformed cubic phase used in traditional fragmentation-based methods. The nanostructure of the phytantriol dispersions was determined to be cubic phase using SAXS whilst GMO dispersions had a reverse hexagonal nanostructure coexisting with cubic phase. The greatest entrapment of Ova was within phytantriol cubosomes prepared from liquid precursors. Release of Ova from the various formulations was sustained; however, release was significantly faster and the extent of release was greater from fragmented dispersions compared to liquid precursor formulations. Taken together, these results suggest that phytantriol cubosomes can be prepared using liquid precursors and that it is a suitable alternative to GMO. Furthermore, the high entrapment and the slow release of Ova in vitro highlight the potential of phytantriol cubosomes prepared using liquid precursors as a novel vaccine delivery system.

AB - Different delivery strategies to improve the immunogenicity of peptide/protein-based vaccines are currently under investigation. In this study, the preparation and physicochemical characterisation of cubosomes, a novel lipid-based particulate system currently being explored for vaccine delivery, was investigated. Cubosomes were prepared from a liquid precursor mixture containing phytantriol or glycerylmonooleate (GMO), F127 for particle stabilisation, and a hydrotrope (ethanol or polyethylene glycol (PEG 200) or propylene glycol (PG)). Several liquid precursors were prepared, and the effect of varying the concentrations of F127 and the hydrotrope on cubosome formation was investigated. Formulations were prepared by fragmentation for comparison. The model protein ovalbumin (Ova) was also entrapped within selected formulations. Submicron-sized particles (180-300 nm) were formed spontaneously upon dilution of the liquid precursors, circumventing the need for the preformed cubic phase used in traditional fragmentation-based methods. The nanostructure of the phytantriol dispersions was determined to be cubic phase using SAXS whilst GMO dispersions had a reverse hexagonal nanostructure coexisting with cubic phase. The greatest entrapment of Ova was within phytantriol cubosomes prepared from liquid precursors. Release of Ova from the various formulations was sustained; however, release was significantly faster and the extent of release was greater from fragmented dispersions compared to liquid precursor formulations. Taken together, these results suggest that phytantriol cubosomes can be prepared using liquid precursors and that it is a suitable alternative to GMO. Furthermore, the high entrapment and the slow release of Ova in vitro highlight the potential of phytantriol cubosomes prepared using liquid precursors as a novel vaccine delivery system.

KW - Cubosomes

KW - Glyceryl monooleate

KW - Liquid precursor

KW - Phytantriol

KW - Proteins

KW - Vaccines

UR - http://www.scopus.com/inward/record.url?scp=80052271341&partnerID=8YFLogxK

U2 - 10.1016/j.ejpb.2010.12.034

DO - 10.1016/j.ejpb.2010.12.034

M3 - Journal article

C2 - 21237267

AN - SCOPUS:80052271341

VL - 79

SP - 15

EP - 22

JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

SN - 0939-6411

IS - 1

ER -

ID: 299416171