Preparation and characterization of insulin-surfactant complexes for loading into lipid-based drug delivery systems
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Preparation and characterization of insulin-surfactant complexes for loading into lipid-based drug delivery systems. / Li, Ping; Nielsen, Hanne Mørck; Fano, Mathias; Müllertz, Anette.
In: Journal of Pharmaceutical Sciences, Vol. 102, No. 8, 08.2013, p. 2689-98.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Preparation and characterization of insulin-surfactant complexes for loading into lipid-based drug delivery systems
AU - Li, Ping
AU - Nielsen, Hanne Mørck
AU - Fano, Mathias
AU - Müllertz, Anette
N1 - Copyright © 2013 Wiley Periodicals, Inc.
PY - 2013/8
Y1 - 2013/8
N2 - Insulin suffers from poor oral bioavailability, but lipid-based drug delivery systems (DDS) may constitute promising tools for improving this. Loading of protein drugs into lipid matrices may, however, be challenging, and different formulation approaches must be taken to achieve sufficient loading and preservation of native structure. The aim of the present study was to characterize insulin after complexation with biocompatible surfactants to improve loading into lipid-based DDS. Insulin-surfactant complexes were prepared by freeze-drying with distearyldimethylammonium bromide or soybean phospholipid as complexing surfactant and dimethyl sulfoxide (DMSO) as solvent. Significant change in secondary structure of insulin freeze dried from DMSO was observed using Fourier transform infrared spectroscopy. Changes were quantitatively smaller in the presence of surfactants, demonstrating both a stabilizing effect of surfactants, but also a nonnative secondary structure in the solid state. Finally, circular dichroism analysis of rehydrated complexes showed that the processing did not irreversibly alter the secondary structure of insulin. In short, the present study demonstrates changes in the secondary structure of insulin after freeze-drying from DMSO, constituting a potential generic issue with this technique for protein processing. In the specific case of insulin, the changes were found to be reversible, explaining the success of this strategy in previous studies.
AB - Insulin suffers from poor oral bioavailability, but lipid-based drug delivery systems (DDS) may constitute promising tools for improving this. Loading of protein drugs into lipid matrices may, however, be challenging, and different formulation approaches must be taken to achieve sufficient loading and preservation of native structure. The aim of the present study was to characterize insulin after complexation with biocompatible surfactants to improve loading into lipid-based DDS. Insulin-surfactant complexes were prepared by freeze-drying with distearyldimethylammonium bromide or soybean phospholipid as complexing surfactant and dimethyl sulfoxide (DMSO) as solvent. Significant change in secondary structure of insulin freeze dried from DMSO was observed using Fourier transform infrared spectroscopy. Changes were quantitatively smaller in the presence of surfactants, demonstrating both a stabilizing effect of surfactants, but also a nonnative secondary structure in the solid state. Finally, circular dichroism analysis of rehydrated complexes showed that the processing did not irreversibly alter the secondary structure of insulin. In short, the present study demonstrates changes in the secondary structure of insulin after freeze-drying from DMSO, constituting a potential generic issue with this technique for protein processing. In the specific case of insulin, the changes were found to be reversible, explaining the success of this strategy in previous studies.
KW - Dimethyl Sulfoxide
KW - Drug Carriers
KW - Freeze Drying
KW - Hypoglycemic Agents
KW - Insulin
KW - Lipids
KW - Protein Structure, Secondary
KW - Spectroscopy, Fourier Transform Infrared
KW - Surface-Active Agents
U2 - 10.1002/jps.23640
DO - 10.1002/jps.23640
M3 - Journal article
C2 - 23839923
VL - 102
SP - 2689
EP - 2698
JO - Journal of Pharmaceutical Sciences
JF - Journal of Pharmaceutical Sciences
SN - 0022-3549
IS - 8
ER -
ID: 104939134