Oral insulin delivery using nanoparticles based on microemulsions with different structure-types: optimisation and in vivo evaluation

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Standard

Oral insulin delivery using nanoparticles based on microemulsions with different structure-types : optimisation and in vivo evaluation. / Graf, Anja; Rades, Thomas; Hook, Sarah M.

In: European Journal of Pharmaceutical Sciences, Vol. 37, No. 1, 2009, p. 53-61.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Graf, A, Rades, T & Hook, SM 2009, 'Oral insulin delivery using nanoparticles based on microemulsions with different structure-types: optimisation and in vivo evaluation', European Journal of Pharmaceutical Sciences, vol. 37, no. 1, pp. 53-61. https://doi.org/10.1016/j.ejps.2008.12.017

APA

Graf, A., Rades, T., & Hook, S. M. (2009). Oral insulin delivery using nanoparticles based on microemulsions with different structure-types: optimisation and in vivo evaluation. European Journal of Pharmaceutical Sciences, 37(1), 53-61. https://doi.org/10.1016/j.ejps.2008.12.017

Vancouver

Graf A, Rades T, Hook SM. Oral insulin delivery using nanoparticles based on microemulsions with different structure-types: optimisation and in vivo evaluation. European Journal of Pharmaceutical Sciences. 2009;37(1):53-61. https://doi.org/10.1016/j.ejps.2008.12.017

Author

Graf, Anja ; Rades, Thomas ; Hook, Sarah M. / Oral insulin delivery using nanoparticles based on microemulsions with different structure-types : optimisation and in vivo evaluation. In: European Journal of Pharmaceutical Sciences. 2009 ; Vol. 37, No. 1. pp. 53-61.

Bibtex

@article{f58df1f623314c46bf6c9838f6f322f1,
title = "Oral insulin delivery using nanoparticles based on microemulsions with different structure-types: optimisation and in vivo evaluation",
abstract = "The purpose of this study was to optimise entrapment of insulin in poly(alkylcyanoacrylate) nanoparticles prepared from microemulsions with different microstructure containing isopropyl myristate, caprylocaproyl macrogolglycerides, polyglyceryl oleate and insulin solution and to investigate the in vitro release and bioactivity of insulin in nanoparticles dispersed in the microemulsion templates. Entrapment efficiency and release of insulin were studied using a reverse-phase HPLC assay. Morphology of the nanoparticles was examined with scanning electron microscopy. Bioactivity of insulin was studied using a streptozotocin-diabetic rat model. Nanoparticles were spherical with 200-400 nm in size without significant difference between different microemulsion templates, types and amounts of monomer. Entrapment efficiency increased significantly with increasing monomer concentration but decreased with increasing aqueous fraction in the microemulsion template. Insulin loading however, showed an opposite trend. In vitro release profiles of insulin from the nanoparticles dispersed in the microemulsion templates were controlled by the monomer concentration only. In vivo, a consistent and significant hypoglycemic effect over controls was found for up to 36 h depending on the type of monomer. No significant serum insulin levels were detectable. This study showed that the strategy of delivering insulin orally, entrapped in nanoparticles and dispersed in a biocompatible microemulsion is promising and highlights the importance of optimisation studies in combination with in vivo experiments.",
author = "Anja Graf and Thomas Rades and Hook, {Sarah M}",
year = "2009",
doi = "10.1016/j.ejps.2008.12.017",
language = "English",
volume = "37",
pages = "53--61",
journal = "Norvegica Pharmaceutica Acta",
issn = "0928-0987",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Oral insulin delivery using nanoparticles based on microemulsions with different structure-types

T2 - optimisation and in vivo evaluation

AU - Graf, Anja

AU - Rades, Thomas

AU - Hook, Sarah M

PY - 2009

Y1 - 2009

N2 - The purpose of this study was to optimise entrapment of insulin in poly(alkylcyanoacrylate) nanoparticles prepared from microemulsions with different microstructure containing isopropyl myristate, caprylocaproyl macrogolglycerides, polyglyceryl oleate and insulin solution and to investigate the in vitro release and bioactivity of insulin in nanoparticles dispersed in the microemulsion templates. Entrapment efficiency and release of insulin were studied using a reverse-phase HPLC assay. Morphology of the nanoparticles was examined with scanning electron microscopy. Bioactivity of insulin was studied using a streptozotocin-diabetic rat model. Nanoparticles were spherical with 200-400 nm in size without significant difference between different microemulsion templates, types and amounts of monomer. Entrapment efficiency increased significantly with increasing monomer concentration but decreased with increasing aqueous fraction in the microemulsion template. Insulin loading however, showed an opposite trend. In vitro release profiles of insulin from the nanoparticles dispersed in the microemulsion templates were controlled by the monomer concentration only. In vivo, a consistent and significant hypoglycemic effect over controls was found for up to 36 h depending on the type of monomer. No significant serum insulin levels were detectable. This study showed that the strategy of delivering insulin orally, entrapped in nanoparticles and dispersed in a biocompatible microemulsion is promising and highlights the importance of optimisation studies in combination with in vivo experiments.

AB - The purpose of this study was to optimise entrapment of insulin in poly(alkylcyanoacrylate) nanoparticles prepared from microemulsions with different microstructure containing isopropyl myristate, caprylocaproyl macrogolglycerides, polyglyceryl oleate and insulin solution and to investigate the in vitro release and bioactivity of insulin in nanoparticles dispersed in the microemulsion templates. Entrapment efficiency and release of insulin were studied using a reverse-phase HPLC assay. Morphology of the nanoparticles was examined with scanning electron microscopy. Bioactivity of insulin was studied using a streptozotocin-diabetic rat model. Nanoparticles were spherical with 200-400 nm in size without significant difference between different microemulsion templates, types and amounts of monomer. Entrapment efficiency increased significantly with increasing monomer concentration but decreased with increasing aqueous fraction in the microemulsion template. Insulin loading however, showed an opposite trend. In vitro release profiles of insulin from the nanoparticles dispersed in the microemulsion templates were controlled by the monomer concentration only. In vivo, a consistent and significant hypoglycemic effect over controls was found for up to 36 h depending on the type of monomer. No significant serum insulin levels were detectable. This study showed that the strategy of delivering insulin orally, entrapped in nanoparticles and dispersed in a biocompatible microemulsion is promising and highlights the importance of optimisation studies in combination with in vivo experiments.

U2 - 10.1016/j.ejps.2008.12.017

DO - 10.1016/j.ejps.2008.12.017

M3 - Journal article

C2 - 19167488

VL - 37

SP - 53

EP - 61

JO - Norvegica Pharmaceutica Acta

JF - Norvegica Pharmaceutica Acta

SN - 0928-0987

IS - 1

ER -

ID: 40353247